Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1125 and Pf6N2E2_1126 are separated by 32 nucleotides Pf6N2E2_1126 and Pf6N2E2_1127 overlap by 4 nucleotides Pf6N2E2_1127 and Pf6N2E2_1128 overlap by 10 nucleotides Pf6N2E2_1128 and Pf6N2E2_1129 overlap by 4 nucleotides
Pf6N2E2_1125 - Transcriptional regulator, AraC family, at 1,320,921 to 1,321,817
_1125
Pf6N2E2_1126 - Two-component system regulatory protein, at 1,321,850 to 1,322,317
_1126
Pf6N2E2_1127 - Sensory box histidine kinase/response regulator, at 1,322,314 to 1,324,632
_1127
Pf6N2E2_1128 - Two-component response regulator, at 1,324,623 to 1,325,291
_1128
Pf6N2E2_1129 - Sensor histidine kinase, at 1,325,288 to 1,326,472
_1129
Position (kb)
1322
1323
1324
1325 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1321.484 kb on - strand, within Pf6N2E2_1125 at 1321.537 kb on + strand, within Pf6N2E2_1125 at 1321.538 kb on - strand, within Pf6N2E2_1125 at 1321.927 kb on + strand, within Pf6N2E2_1126 at 1321.927 kb on + strand, within Pf6N2E2_1126 at 1321.929 kb on + strand, within Pf6N2E2_1126 at 1321.929 kb on + strand, within Pf6N2E2_1126 at 1321.929 kb on + strand, within Pf6N2E2_1126 at 1321.929 kb on + strand, within Pf6N2E2_1126 at 1321.930 kb on - strand, within Pf6N2E2_1126 at 1321.930 kb on - strand, within Pf6N2E2_1126 at 1321.930 kb on - strand, within Pf6N2E2_1126 at 1322.080 kb on - strand, within Pf6N2E2_1126 at 1322.330 kb on - strand at 1322.333 kb on + strand at 1322.333 kb on + strand at 1322.334 kb on - strand at 1322.365 kb on - strand at 1322.370 kb on + strand at 1322.370 kb on + strand at 1322.370 kb on + strand at 1322.370 kb on + strand at 1322.371 kb on - strand at 1322.371 kb on - strand at 1322.371 kb on - strand at 1322.371 kb on - strand at 1322.371 kb on - strand at 1322.749 kb on + strand, within Pf6N2E2_1127 at 1322.749 kb on + strand, within Pf6N2E2_1127 at 1322.749 kb on + strand, within Pf6N2E2_1127 at 1323.026 kb on + strand, within Pf6N2E2_1127 at 1323.026 kb on + strand, within Pf6N2E2_1127 at 1323.294 kb on + strand, within Pf6N2E2_1127 at 1323.295 kb on - strand, within Pf6N2E2_1127 at 1323.533 kb on + strand, within Pf6N2E2_1127 at 1323.533 kb on + strand, within Pf6N2E2_1127 at 1323.605 kb on + strand, within Pf6N2E2_1127 at 1323.605 kb on + strand, within Pf6N2E2_1127 at 1323.605 kb on + strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.671 kb on + strand, within Pf6N2E2_1127 at 1323.672 kb on - strand, within Pf6N2E2_1127 at 1323.672 kb on - strand, within Pf6N2E2_1127 at 1323.714 kb on - strand, within Pf6N2E2_1127 at 1323.756 kb on - strand, within Pf6N2E2_1127 at 1323.899 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.397 kb on + strand, within Pf6N2E2_1127 at 1324.405 kb on + strand at 1324.405 kb on + strand at 1324.405 kb on + strand at 1324.406 kb on - strand at 1324.435 kb on - strand at 1324.516 kb on - strand at 1324.517 kb on + strand at 1324.517 kb on + strand at 1324.517 kb on + strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.726 kb on - strand, within Pf6N2E2_1128 at 1324.982 kb on - strand, within Pf6N2E2_1128 at 1325.231 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,321,484 - Pf6N2E2_1125 0.63 -1.1 1,321,537 + Pf6N2E2_1125 0.69 +1.4 1,321,538 - Pf6N2E2_1125 0.69 +0.9 1,321,927 + Pf6N2E2_1126 0.16 +0.8 1,321,927 + Pf6N2E2_1126 0.16 +0.1 1,321,929 + Pf6N2E2_1126 0.17 -0.4 1,321,929 + Pf6N2E2_1126 0.17 -2.0 1,321,929 + Pf6N2E2_1126 0.17 +0.0 1,321,929 + Pf6N2E2_1126 0.17 -1.5 1,321,930 - Pf6N2E2_1126 0.17 -0.2 1,321,930 - Pf6N2E2_1126 0.17 +1.0 1,321,930 - Pf6N2E2_1126 0.17 +1.5 1,322,080 - Pf6N2E2_1126 0.49 +0.1 1,322,330 - +0.1 1,322,333 + -0.3 1,322,333 + +0.1 1,322,334 - -0.2 1,322,365 - +0.9 1,322,370 + -0.6 1,322,370 + +0.2 1,322,370 + -0.4 1,322,370 + -1.1 1,322,371 - -1.3 1,322,371 - -2.9 1,322,371 - -1.0 1,322,371 - -1.2 1,322,371 - +0.2 1,322,749 + Pf6N2E2_1127 0.19 +0.3 1,322,749 + Pf6N2E2_1127 0.19 -0.8 1,322,749 + Pf6N2E2_1127 0.19 +0.3 1,323,026 + Pf6N2E2_1127 0.31 +0.5 1,323,026 + Pf6N2E2_1127 0.31 +1.7 1,323,294 + Pf6N2E2_1127 0.42 -0.7 1,323,295 - Pf6N2E2_1127 0.42 +0.4 1,323,533 + Pf6N2E2_1127 0.53 +1.4 1,323,533 + Pf6N2E2_1127 0.53 +0.5 1,323,605 + Pf6N2E2_1127 0.56 -0.2 1,323,605 + Pf6N2E2_1127 0.56 -2.2 1,323,605 + Pf6N2E2_1127 0.56 +0.4 1,323,606 - Pf6N2E2_1127 0.56 +0.4 1,323,606 - Pf6N2E2_1127 0.56 -0.6 1,323,606 - Pf6N2E2_1127 0.56 +0.7 1,323,606 - Pf6N2E2_1127 0.56 -2.8 1,323,606 - Pf6N2E2_1127 0.56 -0.6 1,323,606 - Pf6N2E2_1127 0.56 -1.3 1,323,606 - Pf6N2E2_1127 0.56 +1.4 1,323,606 - Pf6N2E2_1127 0.56 -1.0 1,323,671 + Pf6N2E2_1127 0.59 +1.1 1,323,672 - Pf6N2E2_1127 0.59 -1.1 1,323,672 - Pf6N2E2_1127 0.59 -0.2 1,323,714 - Pf6N2E2_1127 0.60 +0.2 1,323,756 - Pf6N2E2_1127 0.62 +0.9 1,323,899 - Pf6N2E2_1127 0.68 +1.8 1,324,362 - Pf6N2E2_1127 0.88 +0.8 1,324,362 - Pf6N2E2_1127 0.88 -0.2 1,324,362 - Pf6N2E2_1127 0.88 -0.5 1,324,397 + Pf6N2E2_1127 0.90 +0.7 1,324,405 + -1.0 1,324,405 + +0.8 1,324,405 + -0.3 1,324,406 - +3.2 1,324,435 - +0.0 1,324,516 - -0.9 1,324,517 + +0.8 1,324,517 + +0.2 1,324,517 + +0.9 1,324,518 - +1.1 1,324,518 - +0.0 1,324,518 - -0.1 1,324,518 - +1.4 1,324,518 - +1.1 1,324,726 - Pf6N2E2_1128 0.15 -0.6 1,324,982 - Pf6N2E2_1128 0.54 -2.5 1,325,231 + -0.2
Or see this region's nucleotide sequence