Experiment: Recovery broth with Azlocillin 2e-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13895 and BBR_RS13900 overlap by 11 nucleotides BBR_RS13900 and BBR_RS13905 are separated by 28 nucleotides BBR_RS13905 and BBR_RS13910 overlap by 4 nucleotides
BBR_RS13895: BBR_RS13895 - acetylglutamate kinase, at 897,400 to 898,356
_RS13895
BBR_RS13900: BBR_RS13900 - acetylornithine transaminase, at 898,346 to 899,641
_RS13900
BBR_RS13905: BBR_RS13905 - ornithine carbamoyltransferase, at 899,670 to 900,635
_RS13905
BBR_RS13910: BBR_RS13910 - arginine repressor, at 900,632 to 901,144
_RS13910
Position (kb) 898
899
900 Strain fitness (log2 ratio) -1
0
1
2 at 897.352 kb on + strand at 897.360 kb on - strand at 897.409 kb on + strand at 897.475 kb on - strand at 897.492 kb on + strand at 897.514 kb on + strand, within BBR_RS13895 at 897.585 kb on + strand, within BBR_RS13895 at 897.667 kb on + strand, within BBR_RS13895 at 897.668 kb on - strand, within BBR_RS13895 at 897.697 kb on + strand, within BBR_RS13895 at 897.742 kb on + strand, within BBR_RS13895 at 897.775 kb on + strand, within BBR_RS13895 at 897.834 kb on + strand, within BBR_RS13895 at 897.880 kb on + strand, within BBR_RS13895 at 897.918 kb on - strand, within BBR_RS13895 at 897.984 kb on + strand, within BBR_RS13895 at 898.000 kb on - strand, within BBR_RS13895 at 898.130 kb on + strand, within BBR_RS13895 at 898.225 kb on - strand, within BBR_RS13895 at 898.250 kb on + strand, within BBR_RS13895 at 898.278 kb on + strand at 898.330 kb on + strand at 898.340 kb on + strand at 898.350 kb on + strand at 898.407 kb on + strand at 898.407 kb on + strand at 898.534 kb on + strand, within BBR_RS13900 at 898.555 kb on + strand, within BBR_RS13900 at 898.589 kb on + strand, within BBR_RS13900 at 898.671 kb on + strand, within BBR_RS13900 at 898.735 kb on + strand, within BBR_RS13900 at 898.758 kb on + strand, within BBR_RS13900 at 898.758 kb on + strand, within BBR_RS13900 at 898.763 kb on + strand, within BBR_RS13900 at 898.788 kb on + strand, within BBR_RS13900 at 898.871 kb on + strand, within BBR_RS13900 at 898.871 kb on + strand, within BBR_RS13900 at 898.925 kb on + strand, within BBR_RS13900 at 898.938 kb on - strand, within BBR_RS13900 at 898.938 kb on - strand, within BBR_RS13900 at 899.010 kb on - strand, within BBR_RS13900 at 899.044 kb on + strand, within BBR_RS13900 at 899.102 kb on + strand, within BBR_RS13900 at 899.102 kb on + strand, within BBR_RS13900 at 899.178 kb on + strand, within BBR_RS13900 at 899.180 kb on + strand, within BBR_RS13900 at 899.180 kb on + strand, within BBR_RS13900 at 899.255 kb on + strand, within BBR_RS13900 at 899.255 kb on + strand, within BBR_RS13900 at 899.279 kb on + strand, within BBR_RS13900 at 899.287 kb on - strand, within BBR_RS13900 at 899.417 kb on + strand, within BBR_RS13900 at 899.424 kb on + strand, within BBR_RS13900 at 899.424 kb on + strand, within BBR_RS13900 at 899.430 kb on - strand, within BBR_RS13900 at 899.430 kb on - strand, within BBR_RS13900 at 899.439 kb on + strand, within BBR_RS13900 at 899.439 kb on + strand, within BBR_RS13900 at 899.449 kb on + strand, within BBR_RS13900 at 899.457 kb on + strand, within BBR_RS13900 at 899.474 kb on + strand, within BBR_RS13900 at 899.489 kb on + strand, within BBR_RS13900 at 899.489 kb on + strand, within BBR_RS13900 at 899.505 kb on - strand, within BBR_RS13900 at 899.535 kb on + strand at 899.550 kb on + strand at 899.672 kb on + strand at 899.674 kb on + strand at 899.674 kb on + strand at 899.674 kb on + strand at 899.674 kb on + strand at 899.748 kb on + strand at 899.925 kb on + strand, within BBR_RS13905 at 899.930 kb on + strand, within BBR_RS13905 at 899.934 kb on + strand, within BBR_RS13905 at 899.934 kb on + strand, within BBR_RS13905 at 900.017 kb on + strand, within BBR_RS13905 at 900.073 kb on + strand, within BBR_RS13905 at 900.089 kb on + strand, within BBR_RS13905 at 900.145 kb on + strand, within BBR_RS13905 at 900.248 kb on + strand, within BBR_RS13905 at 900.298 kb on + strand, within BBR_RS13905 at 900.310 kb on + strand, within BBR_RS13905 at 900.310 kb on + strand, within BBR_RS13905 at 900.410 kb on + strand, within BBR_RS13905 at 900.418 kb on - strand, within BBR_RS13905 at 900.425 kb on + strand, within BBR_RS13905 at 900.425 kb on + strand, within BBR_RS13905 at 900.539 kb on + strand at 900.540 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Azlocillin 2e-05 mM remove 897,352 + -0.8 897,360 - -0.7 897,409 + -0.3 897,475 - -0.1 897,492 + +0.5 897,514 + BBR_RS13895 0.12 +0.4 897,585 + BBR_RS13895 0.19 +0.8 897,667 + BBR_RS13895 0.28 +0.6 897,668 - BBR_RS13895 0.28 +0.7 897,697 + BBR_RS13895 0.31 -0.4 897,742 + BBR_RS13895 0.36 -0.4 897,775 + BBR_RS13895 0.39 +0.7 897,834 + BBR_RS13895 0.45 +0.1 897,880 + BBR_RS13895 0.50 +0.5 897,918 - BBR_RS13895 0.54 -1.6 897,984 + BBR_RS13895 0.61 +0.2 898,000 - BBR_RS13895 0.63 +0.9 898,130 + BBR_RS13895 0.76 -0.6 898,225 - BBR_RS13895 0.86 +1.6 898,250 + BBR_RS13895 0.89 +0.5 898,278 + +0.6 898,330 + +0.1 898,340 + -0.4 898,350 + +0.5 898,407 + -1.5 898,407 + -0.1 898,534 + BBR_RS13900 0.15 -0.3 898,555 + BBR_RS13900 0.16 +0.7 898,589 + BBR_RS13900 0.19 -0.2 898,671 + BBR_RS13900 0.25 -0.1 898,735 + BBR_RS13900 0.30 +0.0 898,758 + BBR_RS13900 0.32 -0.1 898,758 + BBR_RS13900 0.32 -0.7 898,763 + BBR_RS13900 0.32 -1.0 898,788 + BBR_RS13900 0.34 +1.7 898,871 + BBR_RS13900 0.41 -0.0 898,871 + BBR_RS13900 0.41 -0.1 898,925 + BBR_RS13900 0.45 -0.7 898,938 - BBR_RS13900 0.46 -0.3 898,938 - BBR_RS13900 0.46 -0.8 899,010 - BBR_RS13900 0.51 -0.6 899,044 + BBR_RS13900 0.54 +0.4 899,102 + BBR_RS13900 0.58 +0.0 899,102 + BBR_RS13900 0.58 +0.9 899,178 + BBR_RS13900 0.64 -0.4 899,180 + BBR_RS13900 0.64 -0.6 899,180 + BBR_RS13900 0.64 -0.7 899,255 + BBR_RS13900 0.70 +0.2 899,255 + BBR_RS13900 0.70 -0.5 899,279 + BBR_RS13900 0.72 -1.0 899,287 - BBR_RS13900 0.73 +1.4 899,417 + BBR_RS13900 0.83 +0.1 899,424 + BBR_RS13900 0.83 +0.0 899,424 + BBR_RS13900 0.83 +0.3 899,430 - BBR_RS13900 0.84 +0.9 899,430 - BBR_RS13900 0.84 -0.2 899,439 + BBR_RS13900 0.84 -0.3 899,439 + BBR_RS13900 0.84 -0.0 899,449 + BBR_RS13900 0.85 -0.4 899,457 + BBR_RS13900 0.86 -0.0 899,474 + BBR_RS13900 0.87 +0.1 899,489 + BBR_RS13900 0.88 -0.6 899,489 + BBR_RS13900 0.88 +0.8 899,505 - BBR_RS13900 0.89 -0.1 899,535 + +0.1 899,550 + -1.2 899,672 + -0.5 899,674 + -0.0 899,674 + +0.0 899,674 + +0.4 899,674 + -0.7 899,748 + -0.4 899,925 + BBR_RS13905 0.26 +0.7 899,930 + BBR_RS13905 0.27 -0.2 899,934 + BBR_RS13905 0.27 -0.2 899,934 + BBR_RS13905 0.27 +0.1 900,017 + BBR_RS13905 0.36 +0.3 900,073 + BBR_RS13905 0.42 +0.4 900,089 + BBR_RS13905 0.43 +0.2 900,145 + BBR_RS13905 0.49 -1.2 900,248 + BBR_RS13905 0.60 +1.9 900,298 + BBR_RS13905 0.65 +0.2 900,310 + BBR_RS13905 0.66 +0.3 900,310 + BBR_RS13905 0.66 +1.9 900,410 + BBR_RS13905 0.77 +0.5 900,418 - BBR_RS13905 0.77 +1.7 900,425 + BBR_RS13905 0.78 -0.2 900,425 + BBR_RS13905 0.78 +0.5 900,539 + -0.3 900,540 + +0.5
Or see this region's nucleotide sequence