Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1048 and Ac3H11_938 are separated by 67 nucleotides Ac3H11_938 and Ac3H11_939 are separated by 125 nucleotides Ac3H11_939 and Ac3H11_940 are separated by 93 nucleotides
Ac3H11_1048 - tRNA-Leu-TAA, at 58,102 to 58,191
_1048
Ac3H11_938 - FIG00582348: hypothetical protein, at 58,259 to 58,843
_938
Ac3H11_939 - COG1451: Predicted metal-dependent hydrolase, at 58,969 to 59,508
_939
Ac3H11_940 - Glutamate racemase (EC 5.1.1.3), at 59,602 to 60,495
_940
Position (kb) 58
59
60 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 58.196 kb on + strand at 58.217 kb on + strand at 58.217 kb on + strand at 58.218 kb on - strand at 58.218 kb on - strand at 58.218 kb on - strand at 58.218 kb on - strand at 58.316 kb on + strand at 58.316 kb on + strand at 58.358 kb on + strand, within Ac3H11_938 at 58.359 kb on - strand, within Ac3H11_938 at 58.359 kb on - strand, within Ac3H11_938 at 58.359 kb on - strand, within Ac3H11_938 at 58.517 kb on + strand, within Ac3H11_938 at 58.517 kb on + strand, within Ac3H11_938 at 58.518 kb on - strand, within Ac3H11_938 at 58.518 kb on - strand, within Ac3H11_938 at 58.934 kb on + strand at 58.934 kb on + strand at 58.934 kb on + strand at 58.934 kb on + strand at 58.934 kb on + strand at 58.935 kb on - strand at 58.935 kb on - strand at 58.935 kb on - strand at 58.935 kb on - strand at 59.002 kb on + strand at 59.003 kb on - strand at 59.003 kb on - strand at 59.014 kb on + strand at 59.089 kb on + strand, within Ac3H11_939 at 59.089 kb on + strand, within Ac3H11_939 at 59.089 kb on + strand, within Ac3H11_939 at 59.090 kb on - strand, within Ac3H11_939 at 59.128 kb on + strand, within Ac3H11_939 at 59.129 kb on - strand, within Ac3H11_939 at 59.129 kb on - strand, within Ac3H11_939 at 59.129 kb on - strand, within Ac3H11_939 at 59.129 kb on - strand, within Ac3H11_939 at 59.134 kb on + strand, within Ac3H11_939 at 59.134 kb on + strand, within Ac3H11_939 at 59.134 kb on + strand, within Ac3H11_939 at 59.159 kb on - strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.393 kb on - strand, within Ac3H11_939 at 59.393 kb on - strand, within Ac3H11_939 at 59.393 kb on - strand, within Ac3H11_939 at 59.393 kb on - strand, within Ac3H11_939 at 59.393 kb on - strand, within Ac3H11_939 at 59.422 kb on + strand, within Ac3H11_939 at 59.422 kb on + strand, within Ac3H11_939 at 59.431 kb on + strand, within Ac3H11_939 at 59.431 kb on + strand, within Ac3H11_939 at 59.431 kb on + strand, within Ac3H11_939 at 59.432 kb on - strand, within Ac3H11_939 at 59.432 kb on - strand, within Ac3H11_939 at 59.452 kb on + strand, within Ac3H11_939 at 59.452 kb on + strand, within Ac3H11_939 at 59.452 kb on + strand, within Ac3H11_939 at 59.452 kb on + strand, within Ac3H11_939 at 59.453 kb on - strand, within Ac3H11_939 at 59.453 kb on - strand, within Ac3H11_939 at 59.453 kb on - strand, within Ac3H11_939 at 59.453 kb on - strand, within Ac3H11_939 at 59.535 kb on + strand at 59.536 kb on - strand at 59.542 kb on - strand at 59.557 kb on - strand at 59.557 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 58,196 + +0.1 58,217 + -0.5 58,217 + +0.6 58,218 - -0.4 58,218 - -0.1 58,218 - -0.1 58,218 - +0.1 58,316 + -0.4 58,316 + -0.5 58,358 + Ac3H11_938 0.17 +0.7 58,359 - Ac3H11_938 0.17 +0.1 58,359 - Ac3H11_938 0.17 -0.7 58,359 - Ac3H11_938 0.17 -1.2 58,517 + Ac3H11_938 0.44 -0.3 58,517 + Ac3H11_938 0.44 -0.7 58,518 - Ac3H11_938 0.44 -0.1 58,518 - Ac3H11_938 0.44 -1.6 58,934 + -2.0 58,934 + -0.5 58,934 + -0.5 58,934 + +0.1 58,934 + +0.5 58,935 - +0.6 58,935 - +0.4 58,935 - -0.6 58,935 - +0.6 59,002 + +0.4 59,003 - +0.5 59,003 - +0.1 59,014 + +0.2 59,089 + Ac3H11_939 0.22 +0.9 59,089 + Ac3H11_939 0.22 +4.1 59,089 + Ac3H11_939 0.22 -1.1 59,090 - Ac3H11_939 0.22 -0.6 59,128 + Ac3H11_939 0.29 +1.0 59,129 - Ac3H11_939 0.30 +0.9 59,129 - Ac3H11_939 0.30 +0.4 59,129 - Ac3H11_939 0.30 -0.4 59,129 - Ac3H11_939 0.30 -0.2 59,134 + Ac3H11_939 0.31 -1.0 59,134 + Ac3H11_939 0.31 -0.0 59,134 + Ac3H11_939 0.31 -1.3 59,159 - Ac3H11_939 0.35 +0.1 59,392 + Ac3H11_939 0.78 -1.2 59,392 + Ac3H11_939 0.78 -0.8 59,392 + Ac3H11_939 0.78 -0.2 59,392 + Ac3H11_939 0.78 +2.0 59,392 + Ac3H11_939 0.78 +0.3 59,392 + Ac3H11_939 0.78 +0.7 59,392 + Ac3H11_939 0.78 -0.5 59,393 - Ac3H11_939 0.79 +0.3 59,393 - Ac3H11_939 0.79 +0.4 59,393 - Ac3H11_939 0.79 +0.1 59,393 - Ac3H11_939 0.79 +0.3 59,393 - Ac3H11_939 0.79 +0.6 59,422 + Ac3H11_939 0.84 +0.1 59,422 + Ac3H11_939 0.84 -0.3 59,431 + Ac3H11_939 0.86 +0.3 59,431 + Ac3H11_939 0.86 -0.2 59,431 + Ac3H11_939 0.86 +0.0 59,432 - Ac3H11_939 0.86 +0.8 59,432 - Ac3H11_939 0.86 -1.4 59,452 + Ac3H11_939 0.89 -0.3 59,452 + Ac3H11_939 0.89 +0.5 59,452 + Ac3H11_939 0.89 -1.7 59,452 + Ac3H11_939 0.89 -0.4 59,453 - Ac3H11_939 0.90 +0.3 59,453 - Ac3H11_939 0.90 -0.7 59,453 - Ac3H11_939 0.90 -1.4 59,453 - Ac3H11_939 0.90 -1.6 59,535 + -0.2 59,536 - +1.4 59,542 - -0.4 59,557 - +0.2 59,557 - +1.0
Or see this region's nucleotide sequence