Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_929 and Ac3H11_930 are separated by 8 nucleotides Ac3H11_930 and Ac3H11_931 are separated by 321 nucleotides
Ac3H11_929 - L-serine ammonia-lyase (EC 4.3.1.17) (from data) , at 43,184 to 44,584
_929
Ac3H11_930 - hypothetical protein, at 44,593 to 45,345
_930
Ac3H11_931 - Phytoene synthase (EC 2.5.1.32), at 45,667 to 46,611
_931
Position (kb)
44
45
46 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 43.650 kb on + strand, within Ac3H11_929 at 43.650 kb on + strand, within Ac3H11_929 at 43.650 kb on + strand, within Ac3H11_929 at 43.650 kb on + strand, within Ac3H11_929 at 43.650 kb on + strand, within Ac3H11_929 at 43.650 kb on + strand, within Ac3H11_929 at 43.650 kb on + strand, within Ac3H11_929 at 43.651 kb on - strand, within Ac3H11_929 at 43.651 kb on - strand, within Ac3H11_929 at 43.651 kb on - strand, within Ac3H11_929 at 43.651 kb on - strand, within Ac3H11_929 at 43.653 kb on + strand, within Ac3H11_929 at 43.653 kb on + strand, within Ac3H11_929 at 43.654 kb on - strand, within Ac3H11_929 at 43.654 kb on - strand, within Ac3H11_929 at 43.654 kb on - strand, within Ac3H11_929 at 43.654 kb on - strand, within Ac3H11_929 at 43.654 kb on - strand, within Ac3H11_929 at 43.749 kb on + strand, within Ac3H11_929 at 43.750 kb on - strand, within Ac3H11_929 at 44.151 kb on + strand, within Ac3H11_929 at 44.151 kb on + strand, within Ac3H11_929 at 44.151 kb on + strand, within Ac3H11_929 at 44.151 kb on + strand, within Ac3H11_929 at 44.152 kb on - strand, within Ac3H11_929 at 44.152 kb on - strand, within Ac3H11_929 at 44.152 kb on - strand, within Ac3H11_929 at 44.154 kb on + strand, within Ac3H11_929 at 44.154 kb on + strand, within Ac3H11_929 at 44.154 kb on + strand, within Ac3H11_929 at 44.155 kb on - strand, within Ac3H11_929 at 44.441 kb on + strand, within Ac3H11_929 at 44.441 kb on + strand, within Ac3H11_929 at 44.442 kb on - strand, within Ac3H11_929 at 44.442 kb on - strand, within Ac3H11_929 at 44.442 kb on - strand, within Ac3H11_929 at 44.442 kb on - strand, within Ac3H11_929 at 44.647 kb on - strand at 44.675 kb on + strand, within Ac3H11_930 at 44.675 kb on + strand, within Ac3H11_930 at 44.675 kb on + strand, within Ac3H11_930 at 44.675 kb on + strand, within Ac3H11_930 at 44.675 kb on + strand, within Ac3H11_930 at 44.675 kb on + strand, within Ac3H11_930 at 44.675 kb on + strand, within Ac3H11_930 at 44.675 kb on + strand, within Ac3H11_930 at 44.676 kb on - strand, within Ac3H11_930 at 44.676 kb on - strand, within Ac3H11_930 at 44.676 kb on - strand, within Ac3H11_930 at 44.676 kb on - strand, within Ac3H11_930 at 44.774 kb on + strand, within Ac3H11_930 at 44.774 kb on + strand, within Ac3H11_930 at 44.774 kb on + strand, within Ac3H11_930 at 44.775 kb on - strand, within Ac3H11_930 at 44.775 kb on - strand, within Ac3H11_930 at 44.977 kb on + strand, within Ac3H11_930 at 45.071 kb on + strand, within Ac3H11_930 at 45.071 kb on + strand, within Ac3H11_930 at 45.132 kb on - strand, within Ac3H11_930 at 45.373 kb on + strand at 45.374 kb on - strand at 45.421 kb on + strand at 45.421 kb on + strand at 45.422 kb on - strand at 45.457 kb on + strand at 45.458 kb on - strand at 45.458 kb on - strand at 45.467 kb on + strand at 45.489 kb on - strand at 45.668 kb on + strand at 45.668 kb on + strand at 45.668 kb on + strand at 45.668 kb on + strand at 45.669 kb on - strand at 45.669 kb on - strand at 45.669 kb on - strand at 45.669 kb on - strand at 45.669 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 43,650 + Ac3H11_929 0.33 +0.2 43,650 + Ac3H11_929 0.33 +1.3 43,650 + Ac3H11_929 0.33 +0.5 43,650 + Ac3H11_929 0.33 +0.2 43,650 + Ac3H11_929 0.33 +0.5 43,650 + Ac3H11_929 0.33 +0.7 43,650 + Ac3H11_929 0.33 +1.4 43,651 - Ac3H11_929 0.33 -0.8 43,651 - Ac3H11_929 0.33 -0.1 43,651 - Ac3H11_929 0.33 -0.2 43,651 - Ac3H11_929 0.33 -1.1 43,653 + Ac3H11_929 0.33 +0.4 43,653 + Ac3H11_929 0.33 +0.4 43,654 - Ac3H11_929 0.34 +0.2 43,654 - Ac3H11_929 0.34 +0.6 43,654 - Ac3H11_929 0.34 +1.5 43,654 - Ac3H11_929 0.34 -0.1 43,654 - Ac3H11_929 0.34 -1.4 43,749 + Ac3H11_929 0.40 +0.2 43,750 - Ac3H11_929 0.40 -0.4 44,151 + Ac3H11_929 0.69 +1.1 44,151 + Ac3H11_929 0.69 +0.2 44,151 + Ac3H11_929 0.69 -0.2 44,151 + Ac3H11_929 0.69 +1.5 44,152 - Ac3H11_929 0.69 -0.2 44,152 - Ac3H11_929 0.69 -0.1 44,152 - Ac3H11_929 0.69 -0.2 44,154 + Ac3H11_929 0.69 +0.3 44,154 + Ac3H11_929 0.69 -0.9 44,154 + Ac3H11_929 0.69 -0.5 44,155 - Ac3H11_929 0.69 +1.3 44,441 + Ac3H11_929 0.90 +0.2 44,441 + Ac3H11_929 0.90 +0.2 44,442 - Ac3H11_929 0.90 +0.4 44,442 - Ac3H11_929 0.90 +1.1 44,442 - Ac3H11_929 0.90 -0.3 44,442 - Ac3H11_929 0.90 -0.9 44,647 - +0.1 44,675 + Ac3H11_930 0.11 +1.0 44,675 + Ac3H11_930 0.11 -0.6 44,675 + Ac3H11_930 0.11 +0.9 44,675 + Ac3H11_930 0.11 +0.0 44,675 + Ac3H11_930 0.11 -0.0 44,675 + Ac3H11_930 0.11 -2.3 44,675 + Ac3H11_930 0.11 -1.2 44,675 + Ac3H11_930 0.11 +1.0 44,676 - Ac3H11_930 0.11 +0.6 44,676 - Ac3H11_930 0.11 +0.5 44,676 - Ac3H11_930 0.11 +1.3 44,676 - Ac3H11_930 0.11 -0.6 44,774 + Ac3H11_930 0.24 -0.5 44,774 + Ac3H11_930 0.24 +0.2 44,774 + Ac3H11_930 0.24 +0.6 44,775 - Ac3H11_930 0.24 -0.7 44,775 - Ac3H11_930 0.24 -0.0 44,977 + Ac3H11_930 0.51 +0.4 45,071 + Ac3H11_930 0.63 +0.4 45,071 + Ac3H11_930 0.63 +0.0 45,132 - Ac3H11_930 0.72 +0.0 45,373 + -0.4 45,374 - +0.3 45,421 + +0.7 45,421 + -0.6 45,422 - +0.4 45,457 + -0.2 45,458 - -0.2 45,458 - +0.0 45,467 + -0.3 45,489 - -0.0 45,668 + -0.3 45,668 + -0.7 45,668 + -0.5 45,668 + +4.5 45,669 - -0.8 45,669 - -0.9 45,669 - +0.8 45,669 - -0.1 45,669 - -0.1
Or see this region's nucleotide sequence