Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3944 and Ac3H11_3945 are separated by 376 nucleotides Ac3H11_3945 and Ac3H11_3946 overlap by 1 nucleotides Ac3H11_3946 and Ac3H11_3947 overlap by 20 nucleotides Ac3H11_3947 and Ac3H11_3948 overlap by 105 nucleotides
Ac3H11_3944 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 166,426 to 167,424
_3944
Ac3H11_3945 - Sensory histidine kinase QseC, at 167,801 to 169,138
_3945
Ac3H11_3946 - Two-component system response regulator QseB, at 169,138 to 169,797
_3946
Ac3H11_3947 - hypothetical protein, at 169,778 to 169,900
_3947
Ac3H11_3948 - Ferrichrome-iron receptor, at 169,796 to 172,105
_3948
Position (kb) 167
168
169
170 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 166.977 kb on - strand, within Ac3H11_3944 at 167.024 kb on + strand, within Ac3H11_3944 at 167.025 kb on - strand, within Ac3H11_3944 at 167.338 kb on + strand at 167.338 kb on + strand at 167.339 kb on - strand at 167.339 kb on - strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.385 kb on - strand at 167.385 kb on - strand at 167.385 kb on - strand at 167.385 kb on - strand at 167.428 kb on + strand at 167.428 kb on + strand at 167.428 kb on + strand at 167.437 kb on - strand at 167.437 kb on - strand at 167.611 kb on + strand at 167.612 kb on - strand at 167.612 kb on - strand at 167.725 kb on - strand at 167.766 kb on + strand at 167.777 kb on + strand at 167.777 kb on + strand at 167.796 kb on + strand at 167.797 kb on - strand at 167.806 kb on + strand at 168.069 kb on + strand, within Ac3H11_3945 at 168.069 kb on + strand, within Ac3H11_3945 at 168.069 kb on + strand, within Ac3H11_3945 at 168.070 kb on - strand, within Ac3H11_3945 at 168.070 kb on - strand, within Ac3H11_3945 at 168.982 kb on - strand, within Ac3H11_3945 at 168.982 kb on - strand, within Ac3H11_3945 at 169.220 kb on + strand, within Ac3H11_3946 at 169.220 kb on + strand, within Ac3H11_3946 at 169.220 kb on + strand, within Ac3H11_3946 at 169.221 kb on - strand, within Ac3H11_3946 at 169.221 kb on - strand, within Ac3H11_3946 at 169.418 kb on + strand, within Ac3H11_3946 at 169.419 kb on - strand, within Ac3H11_3946 at 169.505 kb on + strand, within Ac3H11_3946 at 169.506 kb on - strand, within Ac3H11_3946 at 169.506 kb on - strand, within Ac3H11_3946 at 169.665 kb on - strand, within Ac3H11_3946 at 169.724 kb on + strand, within Ac3H11_3946 at 169.725 kb on - strand, within Ac3H11_3946 at 169.809 kb on - strand at 169.809 kb on - strand at 169.913 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 166,977 - Ac3H11_3944 0.55 -1.6 167,024 + Ac3H11_3944 0.60 +0.3 167,025 - Ac3H11_3944 0.60 +0.2 167,338 + +0.5 167,338 + -0.7 167,339 - +2.5 167,339 - +0.3 167,384 + -3.0 167,384 + -0.1 167,384 + -0.3 167,384 + +0.1 167,384 + -0.1 167,384 + +0.1 167,384 + +0.2 167,384 + +0.1 167,385 - +0.6 167,385 - +0.3 167,385 - +0.0 167,385 - -0.2 167,428 + -0.0 167,428 + +0.1 167,428 + +0.2 167,437 - -0.2 167,437 - -0.2 167,611 + +0.2 167,612 - -1.1 167,612 - +0.2 167,725 - -0.7 167,766 + -0.7 167,777 + -0.6 167,777 + -0.1 167,796 + -0.4 167,797 - -0.0 167,806 + +0.0 168,069 + Ac3H11_3945 0.20 +0.2 168,069 + Ac3H11_3945 0.20 +0.3 168,069 + Ac3H11_3945 0.20 -0.9 168,070 - Ac3H11_3945 0.20 -0.8 168,070 - Ac3H11_3945 0.20 -1.0 168,982 - Ac3H11_3945 0.88 -0.6 168,982 - Ac3H11_3945 0.88 -0.3 169,220 + Ac3H11_3946 0.12 +1.6 169,220 + Ac3H11_3946 0.12 -0.6 169,220 + Ac3H11_3946 0.12 +1.2 169,221 - Ac3H11_3946 0.13 -1.5 169,221 - Ac3H11_3946 0.13 -0.4 169,418 + Ac3H11_3946 0.42 +0.1 169,419 - Ac3H11_3946 0.43 +2.2 169,505 + Ac3H11_3946 0.56 -0.2 169,506 - Ac3H11_3946 0.56 -0.2 169,506 - Ac3H11_3946 0.56 -0.3 169,665 - Ac3H11_3946 0.80 -0.2 169,724 + Ac3H11_3946 0.89 +0.8 169,725 - Ac3H11_3946 0.89 +0.2 169,809 - +0.1 169,809 - -0.0 169,913 + +0.3
Or see this region's nucleotide sequence