Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3672 and Ac3H11_3673 are separated by 139 nucleotides Ac3H11_3673 and Ac3H11_3674 are separated by 120 nucleotides Ac3H11_3674 and Ac3H11_3675 overlap by 33 nucleotides
Ac3H11_3672 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 20,609 to 21,892
_3672
Ac3H11_3673 - putative exported protein, at 22,032 to 22,358
_3673
Ac3H11_3674 - hypothetical protein, at 22,479 to 22,673
_3674
Ac3H11_3675 - diguanylate cyclase with PAS/PAC sensor, at 22,641 to 24,377
_3675
Position (kb)
22
23 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 21.506 kb on + strand, within Ac3H11_3672 at 21.506 kb on + strand, within Ac3H11_3672 at 21.506 kb on + strand, within Ac3H11_3672 at 21.506 kb on + strand, within Ac3H11_3672 at 21.507 kb on - strand, within Ac3H11_3672 at 21.507 kb on - strand, within Ac3H11_3672 at 21.507 kb on - strand, within Ac3H11_3672 at 21.524 kb on + strand, within Ac3H11_3672 at 21.524 kb on + strand, within Ac3H11_3672 at 21.524 kb on + strand, within Ac3H11_3672 at 21.524 kb on + strand, within Ac3H11_3672 at 21.525 kb on - strand, within Ac3H11_3672 at 21.525 kb on - strand, within Ac3H11_3672 at 21.525 kb on - strand, within Ac3H11_3672 at 21.525 kb on - strand, within Ac3H11_3672 at 21.525 kb on - strand, within Ac3H11_3672 at 21.611 kb on + strand, within Ac3H11_3672 at 21.611 kb on + strand, within Ac3H11_3672 at 21.611 kb on + strand, within Ac3H11_3672 at 21.612 kb on - strand, within Ac3H11_3672 at 21.612 kb on - strand, within Ac3H11_3672 at 21.612 kb on - strand, within Ac3H11_3672 at 21.612 kb on - strand, within Ac3H11_3672 at 21.612 kb on - strand, within Ac3H11_3672 at 21.612 kb on - strand, within Ac3H11_3672 at 21.612 kb on - strand, within Ac3H11_3672 at 21.612 kb on - strand, within Ac3H11_3672 at 21.686 kb on + strand, within Ac3H11_3672 at 21.686 kb on + strand, within Ac3H11_3672 at 21.686 kb on + strand, within Ac3H11_3672 at 21.686 kb on + strand, within Ac3H11_3672 at 21.686 kb on + strand, within Ac3H11_3672 at 21.686 kb on + strand at 21.687 kb on - strand, within Ac3H11_3672 at 21.687 kb on - strand, within Ac3H11_3672 at 21.687 kb on - strand, within Ac3H11_3672 at 21.687 kb on - strand, within Ac3H11_3672 at 21.687 kb on - strand, within Ac3H11_3672 at 21.687 kb on - strand, within Ac3H11_3672 at 21.687 kb on - strand, within Ac3H11_3672 at 21.687 kb on - strand, within Ac3H11_3672 at 21.962 kb on - strand at 21.974 kb on - strand at 22.319 kb on + strand, within Ac3H11_3673 at 22.319 kb on + strand, within Ac3H11_3673 at 22.552 kb on + strand, within Ac3H11_3674 at 22.552 kb on + strand, within Ac3H11_3674 at 22.552 kb on + strand, within Ac3H11_3674 at 22.552 kb on + strand, within Ac3H11_3674 at 22.552 kb on + strand, within Ac3H11_3674 at 22.552 kb on + strand, within Ac3H11_3674 at 22.553 kb on - strand, within Ac3H11_3674 at 22.553 kb on - strand, within Ac3H11_3674 at 22.553 kb on - strand, within Ac3H11_3674 at 22.553 kb on - strand, within Ac3H11_3674 at 22.553 kb on - strand at 22.553 kb on - strand, within Ac3H11_3674 at 22.553 kb on - strand, within Ac3H11_3674 at 22.553 kb on - strand, within Ac3H11_3674 at 22.553 kb on - strand, within Ac3H11_3674 at 22.553 kb on - strand, within Ac3H11_3674 at 22.553 kb on - strand, within Ac3H11_3674 at 22.553 kb on - strand, within Ac3H11_3674 at 22.553 kb on - strand, within Ac3H11_3674 at 22.553 kb on - strand, within Ac3H11_3674 at 22.794 kb on + strand at 22.794 kb on + strand at 22.794 kb on + strand at 22.794 kb on + strand at 22.794 kb on + strand at 22.794 kb on + strand at 22.794 kb on + strand at 22.794 kb on + strand at 22.794 kb on + strand at 22.794 kb on + strand at 22.794 kb on + strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.795 kb on - strand at 22.810 kb on - strand at 22.810 kb on - strand at 22.833 kb on + strand, within Ac3H11_3675 at 22.833 kb on + strand, within Ac3H11_3675 at 22.833 kb on + strand, within Ac3H11_3675 at 22.834 kb on - strand, within Ac3H11_3675 at 22.834 kb on - strand, within Ac3H11_3675 at 22.834 kb on - strand, within Ac3H11_3675 at 22.834 kb on - strand, within Ac3H11_3675 at 22.834 kb on - strand, within Ac3H11_3675 at 22.834 kb on - strand, within Ac3H11_3675 at 22.878 kb on + strand, within Ac3H11_3675 at 22.878 kb on + strand, within Ac3H11_3675 at 22.879 kb on - strand, within Ac3H11_3675 at 22.879 kb on - strand, within Ac3H11_3675 at 22.879 kb on - strand, within Ac3H11_3675 at 22.879 kb on - strand, within Ac3H11_3675 at 22.879 kb on - strand, within Ac3H11_3675 at 22.894 kb on + strand, within Ac3H11_3675 at 22.895 kb on - strand, within Ac3H11_3675 at 22.895 kb on - strand, within Ac3H11_3675 at 22.895 kb on - strand, within Ac3H11_3675 at 22.895 kb on - strand, within Ac3H11_3675 at 22.895 kb on - strand, within Ac3H11_3675 at 22.895 kb on - strand, within Ac3H11_3675 at 22.895 kb on - strand, within Ac3H11_3675 at 22.923 kb on + strand, within Ac3H11_3675 at 22.923 kb on + strand, within Ac3H11_3675 at 22.924 kb on - strand, within Ac3H11_3675 at 22.924 kb on - strand, within Ac3H11_3675 at 22.924 kb on - strand, within Ac3H11_3675 at 22.924 kb on - strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.181 kb on + strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.182 kb on - strand, within Ac3H11_3675 at 23.232 kb on + strand, within Ac3H11_3675 at 23.403 kb on + strand, within Ac3H11_3675 at 23.403 kb on + strand, within Ac3H11_3675 at 23.403 kb on + strand, within Ac3H11_3675 at 23.403 kb on + strand, within Ac3H11_3675 at 23.403 kb on + strand, within Ac3H11_3675 at 23.403 kb on + strand, within Ac3H11_3675 at 23.404 kb on - strand, within Ac3H11_3675 at 23.404 kb on - strand, within Ac3H11_3675 at 23.404 kb on - strand, within Ac3H11_3675 at 23.404 kb on - strand, within Ac3H11_3675 at 23.404 kb on - strand, within Ac3H11_3675 at 23.404 kb on - strand, within Ac3H11_3675 at 23.404 kb on - strand, within Ac3H11_3675 at 23.404 kb on - strand, within Ac3H11_3675 at 23.404 kb on - strand, within Ac3H11_3675 at 23.404 kb on - strand, within Ac3H11_3675 at 23.404 kb on - strand, within Ac3H11_3675 at 23.404 kb on - strand, within Ac3H11_3675 at 23.661 kb on + strand, within Ac3H11_3675 at 23.661 kb on + strand, within Ac3H11_3675 at 23.661 kb on + strand, within Ac3H11_3675
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 21,506 + Ac3H11_3672 0.70 -0.7 21,506 + Ac3H11_3672 0.70 -0.2 21,506 + Ac3H11_3672 0.70 +0.5 21,506 + Ac3H11_3672 0.70 +0.1 21,507 - Ac3H11_3672 0.70 -0.5 21,507 - Ac3H11_3672 0.70 -0.2 21,507 - Ac3H11_3672 0.70 -0.5 21,524 + Ac3H11_3672 0.71 -1.0 21,524 + Ac3H11_3672 0.71 -0.1 21,524 + Ac3H11_3672 0.71 +0.2 21,524 + Ac3H11_3672 0.71 +0.4 21,525 - Ac3H11_3672 0.71 -0.1 21,525 - Ac3H11_3672 0.71 -1.4 21,525 - Ac3H11_3672 0.71 +0.4 21,525 - Ac3H11_3672 0.71 +0.2 21,525 - Ac3H11_3672 0.71 +0.1 21,611 + Ac3H11_3672 0.78 +0.2 21,611 + Ac3H11_3672 0.78 +0.5 21,611 + Ac3H11_3672 0.78 +0.1 21,612 - Ac3H11_3672 0.78 +1.3 21,612 - Ac3H11_3672 0.78 +0.6 21,612 - Ac3H11_3672 0.78 -0.4 21,612 - Ac3H11_3672 0.78 +0.3 21,612 - Ac3H11_3672 0.78 -2.7 21,612 - Ac3H11_3672 0.78 +0.1 21,612 - Ac3H11_3672 0.78 +0.3 21,612 - Ac3H11_3672 0.78 +0.1 21,686 + Ac3H11_3672 0.84 +1.9 21,686 + Ac3H11_3672 0.84 -0.2 21,686 + Ac3H11_3672 0.84 -0.4 21,686 + Ac3H11_3672 0.84 -0.7 21,686 + Ac3H11_3672 0.84 -0.3 21,686 + +1.6 21,687 - Ac3H11_3672 0.84 +1.6 21,687 - Ac3H11_3672 0.84 -0.5 21,687 - Ac3H11_3672 0.84 -1.1 21,687 - Ac3H11_3672 0.84 +0.6 21,687 - Ac3H11_3672 0.84 +0.5 21,687 - Ac3H11_3672 0.84 -0.4 21,687 - Ac3H11_3672 0.84 -0.3 21,687 - Ac3H11_3672 0.84 +0.5 21,962 - +0.1 21,974 - +0.0 22,319 + Ac3H11_3673 0.88 +0.5 22,319 + Ac3H11_3673 0.88 +0.7 22,552 + Ac3H11_3674 0.37 -0.4 22,552 + Ac3H11_3674 0.37 +1.3 22,552 + Ac3H11_3674 0.37 +0.4 22,552 + Ac3H11_3674 0.37 -1.2 22,552 + Ac3H11_3674 0.37 +1.1 22,552 + Ac3H11_3674 0.37 -0.0 22,553 - Ac3H11_3674 0.38 +1.0 22,553 - Ac3H11_3674 0.38 -0.6 22,553 - Ac3H11_3674 0.38 +1.1 22,553 - Ac3H11_3674 0.38 -0.1 22,553 - +1.6 22,553 - Ac3H11_3674 0.38 -0.2 22,553 - Ac3H11_3674 0.38 +0.7 22,553 - Ac3H11_3674 0.38 -0.7 22,553 - Ac3H11_3674 0.38 -0.3 22,553 - Ac3H11_3674 0.38 -0.1 22,553 - Ac3H11_3674 0.38 -0.2 22,553 - Ac3H11_3674 0.38 -0.0 22,553 - Ac3H11_3674 0.38 +5.9 22,553 - Ac3H11_3674 0.38 +1.0 22,794 + +0.7 22,794 + +0.2 22,794 + -0.1 22,794 + -0.9 22,794 + +0.2 22,794 + -0.1 22,794 + -0.7 22,794 + -0.3 22,794 + +0.6 22,794 + +0.7 22,794 + +0.2 22,795 - -1.0 22,795 - +1.1 22,795 - +0.5 22,795 - +0.3 22,795 - -0.3 22,795 - -0.5 22,795 - +0.5 22,795 - -0.1 22,795 - -0.1 22,795 - -0.1 22,795 - +0.3 22,795 - -0.8 22,795 - -0.3 22,795 - +0.8 22,795 - +0.4 22,810 - -0.4 22,810 - +0.1 22,833 + Ac3H11_3675 0.11 +0.0 22,833 + Ac3H11_3675 0.11 +0.2 22,833 + Ac3H11_3675 0.11 -0.3 22,834 - Ac3H11_3675 0.11 +0.1 22,834 - Ac3H11_3675 0.11 -1.0 22,834 - Ac3H11_3675 0.11 -1.1 22,834 - Ac3H11_3675 0.11 -0.2 22,834 - Ac3H11_3675 0.11 +0.8 22,834 - Ac3H11_3675 0.11 +0.4 22,878 + Ac3H11_3675 0.14 +0.2 22,878 + Ac3H11_3675 0.14 -0.2 22,879 - Ac3H11_3675 0.14 +0.7 22,879 - Ac3H11_3675 0.14 -0.6 22,879 - Ac3H11_3675 0.14 -0.9 22,879 - Ac3H11_3675 0.14 -0.2 22,879 - Ac3H11_3675 0.14 -0.7 22,894 + Ac3H11_3675 0.15 +0.1 22,895 - Ac3H11_3675 0.15 +0.9 22,895 - Ac3H11_3675 0.15 -0.5 22,895 - Ac3H11_3675 0.15 -0.5 22,895 - Ac3H11_3675 0.15 -1.0 22,895 - Ac3H11_3675 0.15 +0.6 22,895 - Ac3H11_3675 0.15 +0.3 22,895 - Ac3H11_3675 0.15 -0.6 22,923 + Ac3H11_3675 0.16 -0.0 22,923 + Ac3H11_3675 0.16 -0.3 22,924 - Ac3H11_3675 0.16 -0.8 22,924 - Ac3H11_3675 0.16 +0.6 22,924 - Ac3H11_3675 0.16 -0.0 22,924 - Ac3H11_3675 0.16 -0.6 23,181 + Ac3H11_3675 0.31 +0.3 23,181 + Ac3H11_3675 0.31 -0.3 23,181 + Ac3H11_3675 0.31 -0.8 23,181 + Ac3H11_3675 0.31 -0.7 23,181 + Ac3H11_3675 0.31 +0.0 23,181 + Ac3H11_3675 0.31 -0.7 23,181 + Ac3H11_3675 0.31 +0.1 23,181 + Ac3H11_3675 0.31 -0.8 23,181 + Ac3H11_3675 0.31 +0.6 23,181 + Ac3H11_3675 0.31 -0.4 23,181 + Ac3H11_3675 0.31 +0.0 23,181 + Ac3H11_3675 0.31 +0.4 23,181 + Ac3H11_3675 0.31 +0.0 23,181 + Ac3H11_3675 0.31 -1.4 23,181 + Ac3H11_3675 0.31 +0.2 23,181 + Ac3H11_3675 0.31 -0.4 23,181 + Ac3H11_3675 0.31 -0.3 23,181 + Ac3H11_3675 0.31 -0.1 23,181 + Ac3H11_3675 0.31 +0.8 23,181 + Ac3H11_3675 0.31 -1.1 23,181 + Ac3H11_3675 0.31 -0.4 23,182 - Ac3H11_3675 0.31 -0.2 23,182 - Ac3H11_3675 0.31 +1.7 23,182 - Ac3H11_3675 0.31 +0.5 23,182 - Ac3H11_3675 0.31 -0.4 23,182 - Ac3H11_3675 0.31 -0.4 23,182 - Ac3H11_3675 0.31 +0.2 23,182 - Ac3H11_3675 0.31 -1.2 23,182 - Ac3H11_3675 0.31 -1.1 23,182 - Ac3H11_3675 0.31 -0.4 23,182 - Ac3H11_3675 0.31 +0.8 23,182 - Ac3H11_3675 0.31 -1.0 23,182 - Ac3H11_3675 0.31 +0.7 23,182 - Ac3H11_3675 0.31 -0.4 23,182 - Ac3H11_3675 0.31 -0.6 23,182 - Ac3H11_3675 0.31 -0.1 23,182 - Ac3H11_3675 0.31 -0.9 23,182 - Ac3H11_3675 0.31 +0.4 23,232 + Ac3H11_3675 0.34 +1.6 23,403 + Ac3H11_3675 0.44 +1.1 23,403 + Ac3H11_3675 0.44 +0.3 23,403 + Ac3H11_3675 0.44 +1.4 23,403 + Ac3H11_3675 0.44 -1.1 23,403 + Ac3H11_3675 0.44 +0.2 23,403 + Ac3H11_3675 0.44 +0.7 23,404 - Ac3H11_3675 0.44 +0.4 23,404 - Ac3H11_3675 0.44 +0.3 23,404 - Ac3H11_3675 0.44 -1.8 23,404 - Ac3H11_3675 0.44 +0.3 23,404 - Ac3H11_3675 0.44 -0.6 23,404 - Ac3H11_3675 0.44 +0.5 23,404 - Ac3H11_3675 0.44 +0.0 23,404 - Ac3H11_3675 0.44 +0.8 23,404 - Ac3H11_3675 0.44 +1.2 23,404 - Ac3H11_3675 0.44 +0.5 23,404 - Ac3H11_3675 0.44 +0.7 23,404 - Ac3H11_3675 0.44 -0.4 23,661 + Ac3H11_3675 0.59 +0.6 23,661 + Ac3H11_3675 0.59 +0.6 23,661 + Ac3H11_3675 0.59 +0.5
Or see this region's nucleotide sequence