Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1802 and Ac3H11_1803 are separated by 19 nucleotides Ac3H11_1803 and Ac3H11_1804 are separated by 89 nucleotides Ac3H11_1804 and Ac3H11_1805 are separated by 27 nucleotides
Ac3H11_1802 - HflC protein, at 23,448 to 24,356
_1802
Ac3H11_1803 - Putative inner membrane protein YjeT (clustered with HflC), at 24,376 to 24,564
_1803
Ac3H11_1804 - ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17), at 24,654 to 25,802
_1804
Ac3H11_1805 - Adenylosuccinate synthetase (EC 6.3.4.4), at 25,830 to 27,206
_1805
Position (kb) 24
25
26 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 23.715 kb on + strand, within Ac3H11_1802 at 23.715 kb on + strand, within Ac3H11_1802 at 23.715 kb on + strand, within Ac3H11_1802 at 23.716 kb on - strand, within Ac3H11_1802 at 23.716 kb on - strand, within Ac3H11_1802 at 23.716 kb on - strand, within Ac3H11_1802 at 23.716 kb on - strand, within Ac3H11_1802 at 23.748 kb on + strand, within Ac3H11_1802 at 23.749 kb on - strand, within Ac3H11_1802 at 23.749 kb on - strand, within Ac3H11_1802 at 23.749 kb on - strand, within Ac3H11_1802 at 23.957 kb on + strand, within Ac3H11_1802 at 23.958 kb on - strand, within Ac3H11_1802 at 23.958 kb on - strand, within Ac3H11_1802 at 23.958 kb on - strand, within Ac3H11_1802 at 23.973 kb on + strand, within Ac3H11_1802 at 23.973 kb on + strand, within Ac3H11_1802 at 23.973 kb on + strand, within Ac3H11_1802 at 23.973 kb on + strand, within Ac3H11_1802 at 23.974 kb on - strand, within Ac3H11_1802 at 23.974 kb on - strand, within Ac3H11_1802 at 23.974 kb on - strand, within Ac3H11_1802 at 23.974 kb on - strand, within Ac3H11_1802 at 24.123 kb on + strand, within Ac3H11_1802 at 24.123 kb on + strand, within Ac3H11_1802 at 24.178 kb on - strand, within Ac3H11_1802 at 24.237 kb on + strand, within Ac3H11_1802 at 24.238 kb on - strand, within Ac3H11_1802 at 24.238 kb on - strand, within Ac3H11_1802 at 24.238 kb on - strand, within Ac3H11_1802 at 24.238 kb on - strand, within Ac3H11_1802 at 24.354 kb on + strand at 24.354 kb on + strand at 24.354 kb on + strand at 24.354 kb on + strand at 24.354 kb on + strand at 24.354 kb on + strand at 24.354 kb on + strand at 24.355 kb on - strand at 24.355 kb on - strand at 24.355 kb on - strand at 24.355 kb on - strand at 24.355 kb on - strand at 24.355 kb on - strand at 24.509 kb on + strand, within Ac3H11_1803 at 24.510 kb on - strand at 24.510 kb on - strand, within Ac3H11_1803 at 24.562 kb on + strand at 24.585 kb on + strand at 24.619 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 23,715 + Ac3H11_1802 0.29 -1.9 23,715 + Ac3H11_1802 0.29 -0.4 23,715 + Ac3H11_1802 0.29 -1.8 23,716 - Ac3H11_1802 0.29 -0.6 23,716 - Ac3H11_1802 0.29 -0.7 23,716 - Ac3H11_1802 0.29 -1.4 23,716 - Ac3H11_1802 0.29 -0.1 23,748 + Ac3H11_1802 0.33 -3.0 23,749 - Ac3H11_1802 0.33 -2.6 23,749 - Ac3H11_1802 0.33 -2.4 23,749 - Ac3H11_1802 0.33 -1.4 23,957 + Ac3H11_1802 0.56 -3.1 23,958 - Ac3H11_1802 0.56 -0.4 23,958 - Ac3H11_1802 0.56 -2.0 23,958 - Ac3H11_1802 0.56 -1.8 23,973 + Ac3H11_1802 0.58 -0.3 23,973 + Ac3H11_1802 0.58 -2.0 23,973 + Ac3H11_1802 0.58 -1.5 23,973 + Ac3H11_1802 0.58 +0.6 23,974 - Ac3H11_1802 0.58 -1.4 23,974 - Ac3H11_1802 0.58 -2.2 23,974 - Ac3H11_1802 0.58 -1.3 23,974 - Ac3H11_1802 0.58 -1.0 24,123 + Ac3H11_1802 0.74 -1.6 24,123 + Ac3H11_1802 0.74 -2.3 24,178 - Ac3H11_1802 0.80 -0.2 24,237 + Ac3H11_1802 0.87 -1.0 24,238 - Ac3H11_1802 0.87 -1.3 24,238 - Ac3H11_1802 0.87 -1.6 24,238 - Ac3H11_1802 0.87 -1.4 24,238 - Ac3H11_1802 0.87 -2.0 24,354 + +0.4 24,354 + -0.4 24,354 + +1.0 24,354 + -1.3 24,354 + -0.3 24,354 + +0.6 24,354 + +1.6 24,355 - -0.4 24,355 - +0.9 24,355 - +0.4 24,355 - +1.2 24,355 - -0.0 24,355 - -0.1 24,509 + Ac3H11_1803 0.70 +0.3 24,510 - +1.6 24,510 - Ac3H11_1803 0.71 +0.0 24,562 + -0.9 24,585 + -0.9 24,619 + -0.5
Or see this region's nucleotide sequence