Experiment: Vancomycin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_15985 and AO353_15990 are separated by 96 nucleotides AO353_15990 and AO353_15995 are separated by 61 nucleotides
AO353_15985: AO353_15985 - Alpha,alpha-phosphotrehalase (EC 3.2.1.93) (from data) , at 3,531,673 to 3,533,322
_15985
AO353_15990: AO353_15990 - maltoporin, at 3,533,419 to 3,534,642
_15990
AO353_15995: AO353_15995 - trehalose-specific PTS system, I, HPr, and IIA components (from data) , at 3,534,704 to 3,537,238
_15995
Position (kb)
3533
3534
3535 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3532.614 kb on + strand at 3532.730 kb on - strand, within AO353_15985 at 3532.730 kb on - strand, within AO353_15985 at 3532.730 kb on - strand, within AO353_15985 at 3532.811 kb on - strand, within AO353_15985 at 3532.811 kb on - strand, within AO353_15985 at 3533.117 kb on - strand, within AO353_15985 at 3533.165 kb on - strand at 3533.288 kb on - strand at 3533.432 kb on + strand at 3533.432 kb on + strand at 3533.432 kb on + strand at 3533.432 kb on + strand at 3533.432 kb on + strand at 3533.433 kb on - strand at 3533.433 kb on - strand at 3533.433 kb on - strand at 3533.433 kb on - strand at 3533.439 kb on + strand at 3533.469 kb on - strand at 3533.469 kb on - strand at 3533.584 kb on + strand, within AO353_15990 at 3533.585 kb on - strand, within AO353_15990 at 3533.585 kb on - strand, within AO353_15990 at 3533.611 kb on + strand, within AO353_15990 at 3533.612 kb on - strand, within AO353_15990 at 3533.692 kb on + strand, within AO353_15990 at 3533.692 kb on + strand, within AO353_15990 at 3533.692 kb on + strand, within AO353_15990 at 3533.693 kb on - strand, within AO353_15990 at 3533.693 kb on - strand, within AO353_15990 at 3533.701 kb on + strand, within AO353_15990 at 3533.702 kb on - strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.758 kb on + strand, within AO353_15990 at 3533.759 kb on - strand, within AO353_15990 at 3533.759 kb on - strand, within AO353_15990 at 3533.759 kb on - strand, within AO353_15990 at 3533.759 kb on - strand, within AO353_15990 at 3533.759 kb on - strand, within AO353_15990 at 3533.759 kb on - strand, within AO353_15990 at 3533.759 kb on - strand, within AO353_15990 at 3533.759 kb on - strand, within AO353_15990 at 3533.759 kb on - strand, within AO353_15990 at 3533.759 kb on - strand, within AO353_15990 at 3533.759 kb on - strand, within AO353_15990 at 3533.759 kb on - strand, within AO353_15990 at 3533.777 kb on + strand, within AO353_15990 at 3533.777 kb on + strand, within AO353_15990 at 3533.778 kb on - strand, within AO353_15990 at 3533.778 kb on - strand, within AO353_15990 at 3533.781 kb on + strand, within AO353_15990 at 3533.818 kb on + strand, within AO353_15990 at 3533.821 kb on + strand, within AO353_15990 at 3533.821 kb on + strand, within AO353_15990 at 3533.821 kb on + strand, within AO353_15990 at 3533.822 kb on - strand, within AO353_15990 at 3533.822 kb on - strand, within AO353_15990 at 3533.822 kb on - strand, within AO353_15990 at 3533.822 kb on - strand, within AO353_15990 at 3533.822 kb on - strand, within AO353_15990 at 3533.822 kb on - strand, within AO353_15990 at 3533.822 kb on - strand, within AO353_15990 at 3533.854 kb on + strand, within AO353_15990 at 3533.854 kb on + strand, within AO353_15990 at 3533.854 kb on + strand, within AO353_15990 at 3533.854 kb on + strand, within AO353_15990 at 3533.854 kb on + strand, within AO353_15990 at 3533.854 kb on + strand, within AO353_15990 at 3533.854 kb on + strand, within AO353_15990 at 3533.854 kb on + strand, within AO353_15990 at 3533.855 kb on - strand, within AO353_15990 at 3533.855 kb on - strand, within AO353_15990 at 3533.855 kb on - strand, within AO353_15990 at 3533.855 kb on - strand, within AO353_15990 at 3533.855 kb on - strand, within AO353_15990 at 3533.855 kb on - strand, within AO353_15990 at 3533.855 kb on - strand, within AO353_15990 at 3533.855 kb on - strand, within AO353_15990 at 3533.858 kb on - strand, within AO353_15990 at 3533.858 kb on - strand, within AO353_15990 at 3533.887 kb on - strand, within AO353_15990 at 3533.917 kb on + strand, within AO353_15990 at 3533.917 kb on + strand, within AO353_15990 at 3533.917 kb on + strand, within AO353_15990 at 3533.918 kb on - strand, within AO353_15990 at 3533.918 kb on - strand, within AO353_15990 at 3533.918 kb on - strand, within AO353_15990 at 3533.918 kb on - strand, within AO353_15990 at 3533.924 kb on - strand, within AO353_15990 at 3533.962 kb on + strand, within AO353_15990 at 3533.962 kb on + strand, within AO353_15990 at 3533.962 kb on + strand, within AO353_15990 at 3533.962 kb on + strand, within AO353_15990 at 3533.962 kb on + strand, within AO353_15990 at 3533.962 kb on + strand, within AO353_15990 at 3533.962 kb on + strand, within AO353_15990 at 3533.963 kb on - strand, within AO353_15990 at 3533.963 kb on - strand, within AO353_15990 at 3533.963 kb on - strand, within AO353_15990 at 3533.963 kb on - strand, within AO353_15990 at 3533.963 kb on - strand, within AO353_15990 at 3533.963 kb on - strand, within AO353_15990 at 3533.963 kb on - strand, within AO353_15990 at 3533.963 kb on - strand, within AO353_15990 at 3533.967 kb on + strand, within AO353_15990 at 3534.000 kb on + strand, within AO353_15990 at 3534.037 kb on + strand, within AO353_15990 at 3534.037 kb on + strand, within AO353_15990 at 3534.037 kb on + strand, within AO353_15990 at 3534.037 kb on + strand, within AO353_15990 at 3534.037 kb on + strand, within AO353_15990 at 3534.038 kb on - strand, within AO353_15990 at 3534.038 kb on - strand, within AO353_15990 at 3534.038 kb on - strand, within AO353_15990 at 3534.038 kb on - strand, within AO353_15990 at 3534.038 kb on - strand, within AO353_15990 at 3534.038 kb on - strand, within AO353_15990 at 3534.173 kb on - strand, within AO353_15990 at 3534.173 kb on - strand, within AO353_15990 at 3534.174 kb on + strand, within AO353_15990 at 3534.211 kb on + strand, within AO353_15990 at 3534.211 kb on + strand, within AO353_15990 at 3534.278 kb on - strand, within AO353_15990 at 3534.278 kb on - strand, within AO353_15990 at 3534.278 kb on - strand, within AO353_15990 at 3534.278 kb on - strand, within AO353_15990 at 3534.343 kb on + strand, within AO353_15990 at 3534.343 kb on + strand, within AO353_15990 at 3534.344 kb on - strand, within AO353_15990 at 3534.496 kb on + strand, within AO353_15990 at 3534.496 kb on + strand, within AO353_15990 at 3534.496 kb on + strand, within AO353_15990 at 3534.496 kb on + strand, within AO353_15990 at 3534.496 kb on + strand, within AO353_15990 at 3534.496 kb on + strand, within AO353_15990 at 3534.496 kb on + strand, within AO353_15990 at 3534.497 kb on - strand, within AO353_15990 at 3534.497 kb on - strand, within AO353_15990 at 3534.497 kb on - strand, within AO353_15990 at 3534.499 kb on + strand, within AO353_15990 at 3534.499 kb on + strand, within AO353_15990 at 3534.499 kb on + strand, within AO353_15990 at 3534.499 kb on + strand, within AO353_15990 at 3534.499 kb on + strand, within AO353_15990 at 3534.499 kb on + strand, within AO353_15990 at 3534.500 kb on - strand, within AO353_15990 at 3534.500 kb on - strand, within AO353_15990 at 3534.500 kb on - strand, within AO353_15990 at 3534.500 kb on - strand, within AO353_15990 at 3534.500 kb on - strand, within AO353_15990 at 3534.500 kb on - strand, within AO353_15990 at 3534.500 kb on - strand, within AO353_15990 at 3534.500 kb on - strand, within AO353_15990 at 3534.500 kb on - strand, within AO353_15990 at 3534.500 kb on - strand, within AO353_15990 at 3534.500 kb on - strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.505 kb on + strand, within AO353_15990 at 3534.506 kb on - strand, within AO353_15990 at 3534.506 kb on - strand, within AO353_15990 at 3534.506 kb on - strand, within AO353_15990 at 3534.506 kb on - strand, within AO353_15990 at 3534.506 kb on - strand, within AO353_15990 at 3534.506 kb on - strand, within AO353_15990 at 3534.506 kb on - strand, within AO353_15990 at 3534.506 kb on - strand, within AO353_15990 at 3534.628 kb on + strand at 3534.628 kb on + strand at 3534.640 kb on + strand at 3534.640 kb on + strand at 3534.641 kb on - strand at 3534.641 kb on - strand at 3534.641 kb on - strand at 3534.641 kb on - strand at 3534.862 kb on - strand at 3534.927 kb on + strand at 3534.928 kb on - strand at 3535.040 kb on + strand, within AO353_15995 at 3535.040 kb on + strand, within AO353_15995 at 3535.040 kb on + strand, within AO353_15995 at 3535.040 kb on + strand, within AO353_15995 at 3535.040 kb on + strand, within AO353_15995 at 3535.040 kb on + strand, within AO353_15995 at 3535.040 kb on + strand, within AO353_15995 at 3535.040 kb on + strand, within AO353_15995 at 3535.041 kb on - strand, within AO353_15995 at 3535.041 kb on - strand, within AO353_15995 at 3535.041 kb on - strand, within AO353_15995 at 3535.041 kb on - strand, within AO353_15995 at 3535.315 kb on + strand, within AO353_15995 at 3535.315 kb on + strand, within AO353_15995 at 3535.315 kb on + strand, within AO353_15995 at 3535.315 kb on + strand, within AO353_15995 at 3535.315 kb on + strand, within AO353_15995 at 3535.315 kb on + strand, within AO353_15995 at 3535.316 kb on - strand, within AO353_15995 at 3535.316 kb on - strand, within AO353_15995 at 3535.316 kb on - strand, within AO353_15995 at 3535.316 kb on - strand, within AO353_15995 at 3535.491 kb on + strand, within AO353_15995 at 3535.492 kb on - strand, within AO353_15995 at 3535.594 kb on + strand, within AO353_15995 at 3535.595 kb on - strand, within AO353_15995
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 1 mg/ml remove 3,532,614 + -0.5 3,532,730 - AO353_15985 0.64 +0.6 3,532,730 - AO353_15985 0.64 -0.5 3,532,730 - AO353_15985 0.64 -0.6 3,532,811 - AO353_15985 0.69 -1.4 3,532,811 - AO353_15985 0.69 +0.5 3,533,117 - AO353_15985 0.88 -1.9 3,533,165 - -1.7 3,533,288 - -0.8 3,533,432 + -0.9 3,533,432 + -0.9 3,533,432 + -1.9 3,533,432 + +1.2 3,533,432 + -0.3 3,533,433 - +0.1 3,533,433 - -1.1 3,533,433 - -0.3 3,533,433 - +0.1 3,533,439 + -2.0 3,533,469 - -0.9 3,533,469 - +1.3 3,533,584 + AO353_15990 0.13 -1.5 3,533,585 - AO353_15990 0.14 +0.1 3,533,585 - AO353_15990 0.14 +1.1 3,533,611 + AO353_15990 0.16 +0.1 3,533,612 - AO353_15990 0.16 +0.1 3,533,692 + AO353_15990 0.22 +1.5 3,533,692 + AO353_15990 0.22 -0.3 3,533,692 + AO353_15990 0.22 -0.5 3,533,693 - AO353_15990 0.22 +0.1 3,533,693 - AO353_15990 0.22 +0.3 3,533,701 + AO353_15990 0.23 -0.4 3,533,702 - AO353_15990 0.23 -1.3 3,533,758 + AO353_15990 0.28 -1.1 3,533,758 + AO353_15990 0.28 +0.3 3,533,758 + AO353_15990 0.28 -0.4 3,533,758 + AO353_15990 0.28 -0.6 3,533,758 + AO353_15990 0.28 -0.1 3,533,758 + AO353_15990 0.28 -1.4 3,533,758 + AO353_15990 0.28 -0.4 3,533,758 + AO353_15990 0.28 +0.4 3,533,758 + AO353_15990 0.28 +0.6 3,533,758 + AO353_15990 0.28 +0.1 3,533,758 + AO353_15990 0.28 +3.1 3,533,758 + AO353_15990 0.28 -1.8 3,533,758 + AO353_15990 0.28 +0.7 3,533,758 + AO353_15990 0.28 +0.5 3,533,758 + AO353_15990 0.28 +0.7 3,533,759 - AO353_15990 0.28 -1.9 3,533,759 - AO353_15990 0.28 +1.4 3,533,759 - AO353_15990 0.28 +0.5 3,533,759 - AO353_15990 0.28 +0.6 3,533,759 - AO353_15990 0.28 -0.1 3,533,759 - AO353_15990 0.28 -0.3 3,533,759 - AO353_15990 0.28 -0.3 3,533,759 - AO353_15990 0.28 -0.4 3,533,759 - AO353_15990 0.28 +1.0 3,533,759 - AO353_15990 0.28 +1.1 3,533,759 - AO353_15990 0.28 -1.3 3,533,759 - AO353_15990 0.28 -1.3 3,533,777 + AO353_15990 0.29 +0.1 3,533,777 + AO353_15990 0.29 -1.3 3,533,778 - AO353_15990 0.29 -0.1 3,533,778 - AO353_15990 0.29 -0.1 3,533,781 + AO353_15990 0.30 -1.3 3,533,818 + AO353_15990 0.33 -1.7 3,533,821 + AO353_15990 0.33 +0.7 3,533,821 + AO353_15990 0.33 +1.0 3,533,821 + AO353_15990 0.33 +0.9 3,533,822 - AO353_15990 0.33 -0.3 3,533,822 - AO353_15990 0.33 -1.5 3,533,822 - AO353_15990 0.33 -0.5 3,533,822 - AO353_15990 0.33 -2.2 3,533,822 - AO353_15990 0.33 -0.4 3,533,822 - AO353_15990 0.33 +1.1 3,533,822 - AO353_15990 0.33 -0.1 3,533,854 + AO353_15990 0.36 -0.1 3,533,854 + AO353_15990 0.36 -0.5 3,533,854 + AO353_15990 0.36 -0.2 3,533,854 + AO353_15990 0.36 +0.9 3,533,854 + AO353_15990 0.36 -1.6 3,533,854 + AO353_15990 0.36 +0.9 3,533,854 + AO353_15990 0.36 -1.3 3,533,854 + AO353_15990 0.36 -0.2 3,533,855 - AO353_15990 0.36 -0.1 3,533,855 - AO353_15990 0.36 -0.9 3,533,855 - AO353_15990 0.36 +0.3 3,533,855 - AO353_15990 0.36 +1.3 3,533,855 - AO353_15990 0.36 +0.8 3,533,855 - AO353_15990 0.36 +0.7 3,533,855 - AO353_15990 0.36 +0.8 3,533,855 - AO353_15990 0.36 +0.2 3,533,858 - AO353_15990 0.36 -1.1 3,533,858 - AO353_15990 0.36 +1.5 3,533,887 - AO353_15990 0.38 -0.3 3,533,917 + AO353_15990 0.41 +1.4 3,533,917 + AO353_15990 0.41 -0.3 3,533,917 + AO353_15990 0.41 -1.4 3,533,918 - AO353_15990 0.41 -0.1 3,533,918 - AO353_15990 0.41 +2.2 3,533,918 - AO353_15990 0.41 +0.2 3,533,918 - AO353_15990 0.41 +0.4 3,533,924 - AO353_15990 0.41 -0.8 3,533,962 + AO353_15990 0.44 -1.2 3,533,962 + AO353_15990 0.44 -0.0 3,533,962 + AO353_15990 0.44 +0.1 3,533,962 + AO353_15990 0.44 +1.3 3,533,962 + AO353_15990 0.44 +0.6 3,533,962 + AO353_15990 0.44 -0.8 3,533,962 + AO353_15990 0.44 -0.3 3,533,963 - AO353_15990 0.44 +2.2 3,533,963 - AO353_15990 0.44 +0.9 3,533,963 - AO353_15990 0.44 -0.2 3,533,963 - AO353_15990 0.44 -0.6 3,533,963 - AO353_15990 0.44 -0.2 3,533,963 - AO353_15990 0.44 +0.8 3,533,963 - AO353_15990 0.44 -0.5 3,533,963 - AO353_15990 0.44 +0.0 3,533,967 + AO353_15990 0.45 +0.8 3,534,000 + AO353_15990 0.47 -2.1 3,534,037 + AO353_15990 0.50 +1.0 3,534,037 + AO353_15990 0.50 +0.4 3,534,037 + AO353_15990 0.50 +2.1 3,534,037 + AO353_15990 0.50 +0.3 3,534,037 + AO353_15990 0.50 +0.9 3,534,038 - AO353_15990 0.51 +2.5 3,534,038 - AO353_15990 0.51 +1.2 3,534,038 - AO353_15990 0.51 -0.1 3,534,038 - AO353_15990 0.51 +0.8 3,534,038 - AO353_15990 0.51 +0.3 3,534,038 - AO353_15990 0.51 +0.7 3,534,173 - AO353_15990 0.62 +0.9 3,534,173 - AO353_15990 0.62 +1.1 3,534,174 + AO353_15990 0.62 -2.1 3,534,211 + AO353_15990 0.65 +0.1 3,534,211 + AO353_15990 0.65 -2.2 3,534,278 - AO353_15990 0.70 +1.5 3,534,278 - AO353_15990 0.70 -0.7 3,534,278 - AO353_15990 0.70 -0.5 3,534,278 - AO353_15990 0.70 +0.0 3,534,343 + AO353_15990 0.75 +1.1 3,534,343 + AO353_15990 0.75 -2.4 3,534,344 - AO353_15990 0.76 +0.1 3,534,496 + AO353_15990 0.88 -0.9 3,534,496 + AO353_15990 0.88 -0.5 3,534,496 + AO353_15990 0.88 -0.9 3,534,496 + AO353_15990 0.88 +0.5 3,534,496 + AO353_15990 0.88 -0.0 3,534,496 + AO353_15990 0.88 +1.1 3,534,496 + AO353_15990 0.88 -1.9 3,534,497 - AO353_15990 0.88 +0.1 3,534,497 - AO353_15990 0.88 +0.1 3,534,497 - AO353_15990 0.88 -0.3 3,534,499 + AO353_15990 0.88 +0.8 3,534,499 + AO353_15990 0.88 +1.2 3,534,499 + AO353_15990 0.88 -0.9 3,534,499 + AO353_15990 0.88 -0.1 3,534,499 + AO353_15990 0.88 +1.0 3,534,499 + AO353_15990 0.88 +1.1 3,534,500 - AO353_15990 0.88 -1.3 3,534,500 - AO353_15990 0.88 +0.4 3,534,500 - AO353_15990 0.88 -0.0 3,534,500 - AO353_15990 0.88 +0.0 3,534,500 - AO353_15990 0.88 -0.5 3,534,500 - AO353_15990 0.88 -1.1 3,534,500 - AO353_15990 0.88 +0.1 3,534,500 - AO353_15990 0.88 +0.6 3,534,500 - AO353_15990 0.88 +1.0 3,534,500 - AO353_15990 0.88 +1.3 3,534,500 - AO353_15990 0.88 +0.6 3,534,505 + AO353_15990 0.89 +0.1 3,534,505 + AO353_15990 0.89 +0.6 3,534,505 + AO353_15990 0.89 -2.1 3,534,505 + AO353_15990 0.89 +2.2 3,534,505 + AO353_15990 0.89 +1.2 3,534,505 + AO353_15990 0.89 +0.5 3,534,505 + AO353_15990 0.89 -1.6 3,534,505 + AO353_15990 0.89 +0.9 3,534,505 + AO353_15990 0.89 -0.0 3,534,505 + AO353_15990 0.89 -0.7 3,534,505 + AO353_15990 0.89 -1.1 3,534,505 + AO353_15990 0.89 +2.1 3,534,505 + AO353_15990 0.89 +0.8 3,534,506 - AO353_15990 0.89 -0.6 3,534,506 - AO353_15990 0.89 -0.5 3,534,506 - AO353_15990 0.89 -0.9 3,534,506 - AO353_15990 0.89 +0.4 3,534,506 - AO353_15990 0.89 -0.3 3,534,506 - AO353_15990 0.89 +0.5 3,534,506 - AO353_15990 0.89 +1.0 3,534,506 - AO353_15990 0.89 -0.3 3,534,628 + +0.2 3,534,628 + -0.6 3,534,640 + -0.9 3,534,640 + -1.1 3,534,641 - -0.3 3,534,641 - -1.9 3,534,641 - -0.4 3,534,641 - +0.3 3,534,862 - -0.9 3,534,927 + +0.4 3,534,928 - -0.3 3,535,040 + AO353_15995 0.13 -0.5 3,535,040 + AO353_15995 0.13 -1.4 3,535,040 + AO353_15995 0.13 -1.3 3,535,040 + AO353_15995 0.13 +0.8 3,535,040 + AO353_15995 0.13 +0.8 3,535,040 + AO353_15995 0.13 +0.9 3,535,040 + AO353_15995 0.13 -1.7 3,535,040 + AO353_15995 0.13 -1.1 3,535,041 - AO353_15995 0.13 +1.9 3,535,041 - AO353_15995 0.13 -1.5 3,535,041 - AO353_15995 0.13 +1.5 3,535,041 - AO353_15995 0.13 +0.0 3,535,315 + AO353_15995 0.24 +0.2 3,535,315 + AO353_15995 0.24 -0.9 3,535,315 + AO353_15995 0.24 +1.6 3,535,315 + AO353_15995 0.24 -1.9 3,535,315 + AO353_15995 0.24 -2.5 3,535,315 + AO353_15995 0.24 -0.5 3,535,316 - AO353_15995 0.24 -1.7 3,535,316 - AO353_15995 0.24 -0.8 3,535,316 - AO353_15995 0.24 +0.5 3,535,316 - AO353_15995 0.24 -0.7 3,535,491 + AO353_15995 0.31 +0.8 3,535,492 - AO353_15995 0.31 -1.1 3,535,594 + AO353_15995 0.35 -0.4 3,535,595 - AO353_15995 0.35 -1.7
Or see this region's nucleotide sequence