Experiment: Vancomycin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_05540 and AO353_05545 are separated by 197 nucleotides AO353_05545 and AO353_05550 are separated by 117 nucleotides AO353_05550 and AO353_05555 are separated by 3 nucleotides AO353_05555 and AO353_05560 are separated by 159 nucleotides
AO353_05540: AO353_05540 - polyribonucleotide nucleotidyltransferase, at 1,225,170 to 1,227,275
_05540
AO353_05545: AO353_05545 - 30S ribosomal protein S15, at 1,227,473 to 1,227,742
_05545
AO353_05550: AO353_05550 - pseudouridine synthase, at 1,227,860 to 1,228,777
_05550
AO353_05555: AO353_05555 - ribosome-binding factor A, at 1,228,781 to 1,229,182
_05555
AO353_05560: AO353_05560 - translation initiation factor IF-2, at 1,229,342 to 1,231,858
_05560
Position (kb) 1227
1228
1229 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1227.371 kb on - strand at 1227.371 kb on - strand at 1227.388 kb on - strand at 1228.009 kb on - strand, within AO353_05550 at 1228.113 kb on - strand, within AO353_05550 at 1228.113 kb on - strand, within AO353_05550 at 1228.113 kb on - strand, within AO353_05550 at 1228.113 kb on - strand, within AO353_05550 at 1228.113 kb on - strand, within AO353_05550 at 1228.113 kb on - strand, within AO353_05550 at 1228.113 kb on - strand, within AO353_05550 at 1228.113 kb on - strand, within AO353_05550 at 1228.113 kb on - strand, within AO353_05550 at 1228.172 kb on - strand, within AO353_05550 at 1228.243 kb on - strand, within AO353_05550 at 1228.243 kb on - strand, within AO353_05550 at 1228.243 kb on - strand, within AO353_05550 at 1228.243 kb on - strand, within AO353_05550 at 1228.243 kb on - strand, within AO353_05550 at 1228.243 kb on - strand, within AO353_05550 at 1228.243 kb on - strand, within AO353_05550 at 1228.243 kb on - strand, within AO353_05550 at 1228.243 kb on - strand, within AO353_05550 at 1228.243 kb on - strand, within AO353_05550 at 1228.243 kb on - strand, within AO353_05550 at 1228.316 kb on - strand, within AO353_05550 at 1228.316 kb on - strand, within AO353_05550 at 1228.368 kb on + strand, within AO353_05550 at 1228.368 kb on + strand, within AO353_05550 at 1228.369 kb on - strand, within AO353_05550 at 1228.401 kb on + strand, within AO353_05550 at 1228.402 kb on - strand, within AO353_05550 at 1228.551 kb on + strand, within AO353_05550 at 1228.575 kb on + strand, within AO353_05550 at 1228.575 kb on + strand, within AO353_05550 at 1228.576 kb on - strand, within AO353_05550 at 1228.759 kb on + strand at 1228.760 kb on - strand at 1228.760 kb on - strand at 1228.760 kb on - strand at 1228.782 kb on + strand at 1228.783 kb on - strand at 1228.783 kb on - strand at 1229.153 kb on - strand at 1229.170 kb on - strand at 1229.191 kb on - strand at 1229.191 kb on - strand at 1229.344 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 1 mg/ml remove 1,227,371 - -0.1 1,227,371 - -0.2 1,227,388 - -0.3 1,228,009 - AO353_05550 0.16 +0.1 1,228,113 - AO353_05550 0.28 -2.5 1,228,113 - AO353_05550 0.28 -0.9 1,228,113 - AO353_05550 0.28 +1.1 1,228,113 - AO353_05550 0.28 -0.7 1,228,113 - AO353_05550 0.28 +0.7 1,228,113 - AO353_05550 0.28 -1.5 1,228,113 - AO353_05550 0.28 +1.2 1,228,113 - AO353_05550 0.28 -2.0 1,228,113 - AO353_05550 0.28 -2.1 1,228,172 - AO353_05550 0.34 -1.6 1,228,243 - AO353_05550 0.42 -3.0 1,228,243 - AO353_05550 0.42 -0.5 1,228,243 - AO353_05550 0.42 -0.8 1,228,243 - AO353_05550 0.42 -0.1 1,228,243 - AO353_05550 0.42 +0.3 1,228,243 - AO353_05550 0.42 -1.4 1,228,243 - AO353_05550 0.42 -1.1 1,228,243 - AO353_05550 0.42 -0.5 1,228,243 - AO353_05550 0.42 +0.1 1,228,243 - AO353_05550 0.42 +2.3 1,228,243 - AO353_05550 0.42 -1.3 1,228,316 - AO353_05550 0.50 +1.1 1,228,316 - AO353_05550 0.50 -3.5 1,228,368 + AO353_05550 0.55 -0.5 1,228,368 + AO353_05550 0.55 -1.4 1,228,369 - AO353_05550 0.55 -0.5 1,228,401 + AO353_05550 0.59 +0.0 1,228,402 - AO353_05550 0.59 -3.5 1,228,551 + AO353_05550 0.75 -1.9 1,228,575 + AO353_05550 0.78 -1.1 1,228,575 + AO353_05550 0.78 +0.5 1,228,576 - AO353_05550 0.78 -2.9 1,228,759 + -0.9 1,228,760 - -3.7 1,228,760 - -2.8 1,228,760 - -0.2 1,228,782 + -0.1 1,228,783 - -2.1 1,228,783 - -1.4 1,229,153 - -0.1 1,229,170 - -2.3 1,229,191 - -2.0 1,229,191 - -1.9 1,229,344 - -0.1
Or see this region's nucleotide sequence