Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1022 and Shew_1023 are separated by 146 nucleotides Shew_1023 and Shew_1024 are separated by 1 nucleotides Shew_1024 and Shew_1025 overlap by 4 nucleotides Shew_1025 and Shew_1026 are separated by 115 nucleotides
Shew_1022: Shew_1022 - tyrosyl-tRNA synthetase (RefSeq), at 1,181,413 to 1,182,609
_1022
Shew_1023: Shew_1023 - hypothetical protein (RefSeq), at 1,182,756 to 1,183,235
_1023
Shew_1024: Shew_1024 - hypothetical protein (RefSeq), at 1,183,237 to 1,183,857
_1024
Shew_1025: Shew_1025 - hypothetical protein (RefSeq), at 1,183,854 to 1,184,243
_1025
Shew_1026: Shew_1026 - cobalamin biosynthesis protein CbiB (RefSeq), at 1,184,359 to 1,185,342
_1026
Position (kb)
1183
1184 Strain fitness (log2 ratio)
-1
0
1 at 1182.871 kb on - strand, within Shew_1023 at 1183.044 kb on + strand, within Shew_1023 at 1183.065 kb on - strand, within Shew_1023 at 1183.233 kb on + strand at 1183.373 kb on - strand, within Shew_1024 at 1183.373 kb on - strand, within Shew_1024 at 1183.588 kb on + strand, within Shew_1024 at 1183.750 kb on + strand, within Shew_1024 at 1183.925 kb on + strand, within Shew_1025 at 1183.925 kb on + strand, within Shew_1025 at 1184.490 kb on - strand, within Shew_1026 at 1184.562 kb on - strand, within Shew_1026 at 1184.768 kb on + strand, within Shew_1026
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,182,871 - Shew_1023 0.24 +0.9 1,183,044 + Shew_1023 0.60 +0.4 1,183,065 - Shew_1023 0.64 +0.0 1,183,233 + +0.8 1,183,373 - Shew_1024 0.22 +0.2 1,183,373 - Shew_1024 0.22 +0.1 1,183,588 + Shew_1024 0.57 +0.1 1,183,750 + Shew_1024 0.83 +0.0 1,183,925 + Shew_1025 0.18 +0.4 1,183,925 + Shew_1025 0.18 +0.3 1,184,490 - Shew_1026 0.13 +0.1 1,184,562 - Shew_1026 0.21 -0.2 1,184,768 + Shew_1026 0.42 +0.1
Or see this region's nucleotide sequence