Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_959 and Pf6N2E2_960 are separated by 82 nucleotides
Pf6N2E2_959 - putative membrane protein, at 1,130,027 to 1,130,761
_959
Pf6N2E2_960 - Type IV secretory pathway, VirD4 components, at 1,130,844 to 1,132,964
_960
Position (kb)
1130
1131 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1129.082 kb on + strand at 1129.215 kb on + strand at 1129.797 kb on + strand at 1130.031 kb on - strand at 1130.031 kb on - strand at 1130.137 kb on - strand, within Pf6N2E2_959 at 1130.337 kb on + strand, within Pf6N2E2_959 at 1130.530 kb on - strand, within Pf6N2E2_959 at 1130.582 kb on - strand, within Pf6N2E2_959 at 1130.582 kb on - strand, within Pf6N2E2_959 at 1130.615 kb on + strand, within Pf6N2E2_959 at 1130.615 kb on + strand, within Pf6N2E2_959 at 1130.811 kb on + strand at 1130.873 kb on + strand at 1130.873 kb on + strand at 1131.275 kb on + strand, within Pf6N2E2_960 at 1131.309 kb on + strand, within Pf6N2E2_960 at 1131.309 kb on + strand, within Pf6N2E2_960 at 1131.343 kb on + strand, within Pf6N2E2_960 at 1131.344 kb on - strand, within Pf6N2E2_960 at 1131.503 kb on - strand, within Pf6N2E2_960 at 1131.503 kb on - strand, within Pf6N2E2_960 at 1131.596 kb on - strand, within Pf6N2E2_960 at 1131.665 kb on - strand, within Pf6N2E2_960 at 1131.678 kb on + strand, within Pf6N2E2_960 at 1131.679 kb on - strand, within Pf6N2E2_960 at 1131.748 kb on + strand, within Pf6N2E2_960 at 1131.748 kb on + strand, within Pf6N2E2_960 at 1131.748 kb on + strand, within Pf6N2E2_960 at 1131.752 kb on + strand, within Pf6N2E2_960 at 1131.752 kb on + strand, within Pf6N2E2_960
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,129,082 + +1.3 1,129,215 + +0.6 1,129,797 + +0.9 1,130,031 - +0.5 1,130,031 - -1.0 1,130,137 - Pf6N2E2_959 0.15 -1.1 1,130,337 + Pf6N2E2_959 0.42 -0.2 1,130,530 - Pf6N2E2_959 0.68 -0.4 1,130,582 - Pf6N2E2_959 0.76 -1.7 1,130,582 - Pf6N2E2_959 0.76 -0.1 1,130,615 + Pf6N2E2_959 0.80 -0.3 1,130,615 + Pf6N2E2_959 0.80 -2.2 1,130,811 + +0.7 1,130,873 + -1.0 1,130,873 + +1.0 1,131,275 + Pf6N2E2_960 0.20 +1.3 1,131,309 + Pf6N2E2_960 0.22 +2.8 1,131,309 + Pf6N2E2_960 0.22 -1.3 1,131,343 + Pf6N2E2_960 0.24 +0.6 1,131,344 - Pf6N2E2_960 0.24 +2.4 1,131,503 - Pf6N2E2_960 0.31 -1.0 1,131,503 - Pf6N2E2_960 0.31 -0.2 1,131,596 - Pf6N2E2_960 0.35 +2.2 1,131,665 - Pf6N2E2_960 0.39 -1.6 1,131,678 + Pf6N2E2_960 0.39 +0.1 1,131,679 - Pf6N2E2_960 0.39 +0.8 1,131,748 + Pf6N2E2_960 0.43 +1.5 1,131,748 + Pf6N2E2_960 0.43 +1.0 1,131,748 + Pf6N2E2_960 0.43 -0.4 1,131,752 + Pf6N2E2_960 0.43 -0.4 1,131,752 + Pf6N2E2_960 0.43 +0.4
Or see this region's nucleotide sequence