Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_847 and Pf6N2E2_848 overlap by 8 nucleotides Pf6N2E2_848 and Pf6N2E2_849 overlap by 19 nucleotides Pf6N2E2_849 and Pf6N2E2_850 are separated by 282 nucleotides Pf6N2E2_850 and Pf6N2E2_851 are separated by 96 nucleotides
Pf6N2E2_847 - Phosphoribosyl transferase domain protein, at 992,337 to 993,041
_847
Pf6N2E2_848 - hypothetical protein, at 993,034 to 993,729
_848
Pf6N2E2_849 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 993,711 to 995,078
_849
Pf6N2E2_850 - hypothetical protein, at 995,361 to 995,474
_850
Pf6N2E2_851 - Osmotically inducible protein Y precursor, at 995,571 to 996,218
_851
Position (kb) 993
994
995
996 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 992.798 kb on - strand, within Pf6N2E2_847 at 992.934 kb on + strand, within Pf6N2E2_847 at 992.934 kb on + strand, within Pf6N2E2_847 at 992.935 kb on - strand, within Pf6N2E2_847 at 993.172 kb on + strand, within Pf6N2E2_848 at 993.172 kb on + strand, within Pf6N2E2_848 at 993.173 kb on - strand, within Pf6N2E2_848 at 993.173 kb on - strand, within Pf6N2E2_848 at 993.670 kb on + strand at 993.671 kb on - strand at 993.671 kb on - strand at 993.671 kb on - strand at 993.705 kb on - strand at 993.705 kb on - strand at 993.705 kb on - strand at 993.727 kb on + strand at 993.728 kb on - strand at 993.874 kb on + strand, within Pf6N2E2_849 at 993.874 kb on + strand, within Pf6N2E2_849 at 994.115 kb on - strand, within Pf6N2E2_849 at 994.318 kb on + strand, within Pf6N2E2_849 at 994.319 kb on - strand, within Pf6N2E2_849 at 994.321 kb on + strand, within Pf6N2E2_849 at 994.322 kb on - strand, within Pf6N2E2_849 at 994.322 kb on - strand, within Pf6N2E2_849 at 994.322 kb on - strand, within Pf6N2E2_849 at 994.322 kb on - strand, within Pf6N2E2_849 at 994.483 kb on + strand, within Pf6N2E2_849 at 994.484 kb on - strand, within Pf6N2E2_849 at 994.484 kb on - strand, within Pf6N2E2_849 at 994.484 kb on - strand, within Pf6N2E2_849 at 994.484 kb on - strand, within Pf6N2E2_849 at 994.523 kb on - strand, within Pf6N2E2_849 at 994.570 kb on + strand, within Pf6N2E2_849 at 994.570 kb on + strand, within Pf6N2E2_849 at 994.607 kb on - strand, within Pf6N2E2_849 at 994.771 kb on + strand, within Pf6N2E2_849 at 994.771 kb on + strand, within Pf6N2E2_849 at 994.772 kb on - strand, within Pf6N2E2_849 at 994.772 kb on - strand, within Pf6N2E2_849 at 994.870 kb on - strand, within Pf6N2E2_849 at 994.873 kb on + strand, within Pf6N2E2_849 at 994.873 kb on + strand, within Pf6N2E2_849 at 994.874 kb on - strand, within Pf6N2E2_849 at 994.874 kb on - strand, within Pf6N2E2_849 at 995.018 kb on + strand at 995.019 kb on - strand at 995.029 kb on + strand at 995.030 kb on - strand at 995.041 kb on + strand at 995.125 kb on + strand at 995.125 kb on + strand at 995.125 kb on + strand at 995.125 kb on + strand at 995.126 kb on - strand at 995.126 kb on - strand at 995.126 kb on - strand at 995.215 kb on + strand at 995.219 kb on + strand at 995.219 kb on + strand at 995.219 kb on + strand at 995.220 kb on - strand at 995.225 kb on + strand at 995.226 kb on - strand at 995.290 kb on - strand at 995.297 kb on + strand at 995.323 kb on - strand at 995.362 kb on + strand at 995.739 kb on - strand, within Pf6N2E2_851 at 995.864 kb on - strand, within Pf6N2E2_851 at 995.928 kb on + strand, within Pf6N2E2_851 at 995.929 kb on - strand, within Pf6N2E2_851 at 995.929 kb on - strand, within Pf6N2E2_851 at 996.015 kb on + strand, within Pf6N2E2_851 at 996.015 kb on + strand, within Pf6N2E2_851 at 996.015 kb on + strand, within Pf6N2E2_851 at 996.016 kb on - strand, within Pf6N2E2_851 at 996.016 kb on - strand, within Pf6N2E2_851 at 996.016 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 992,798 - Pf6N2E2_847 0.65 -0.5 992,934 + Pf6N2E2_847 0.85 -0.6 992,934 + Pf6N2E2_847 0.85 -0.2 992,935 - Pf6N2E2_847 0.85 -0.5 993,172 + Pf6N2E2_848 0.20 -2.4 993,172 + Pf6N2E2_848 0.20 -0.2 993,173 - Pf6N2E2_848 0.20 +0.9 993,173 - Pf6N2E2_848 0.20 -2.6 993,670 + -0.9 993,671 - +0.1 993,671 - -0.9 993,671 - -0.8 993,705 - +1.0 993,705 - +0.6 993,705 - +0.1 993,727 + +2.2 993,728 - -0.4 993,874 + Pf6N2E2_849 0.12 -0.2 993,874 + Pf6N2E2_849 0.12 -0.6 994,115 - Pf6N2E2_849 0.30 +0.8 994,318 + Pf6N2E2_849 0.44 +0.3 994,319 - Pf6N2E2_849 0.44 -2.5 994,321 + Pf6N2E2_849 0.45 -1.0 994,322 - Pf6N2E2_849 0.45 +2.3 994,322 - Pf6N2E2_849 0.45 +0.5 994,322 - Pf6N2E2_849 0.45 -0.9 994,322 - Pf6N2E2_849 0.45 -0.7 994,483 + Pf6N2E2_849 0.56 -0.1 994,484 - Pf6N2E2_849 0.57 -0.7 994,484 - Pf6N2E2_849 0.57 +1.2 994,484 - Pf6N2E2_849 0.57 +2.6 994,484 - Pf6N2E2_849 0.57 -0.3 994,523 - Pf6N2E2_849 0.59 +0.3 994,570 + Pf6N2E2_849 0.63 +0.2 994,570 + Pf6N2E2_849 0.63 +1.0 994,607 - Pf6N2E2_849 0.65 +0.7 994,771 + Pf6N2E2_849 0.77 +1.0 994,771 + Pf6N2E2_849 0.77 +0.3 994,772 - Pf6N2E2_849 0.78 +1.4 994,772 - Pf6N2E2_849 0.78 +1.1 994,870 - Pf6N2E2_849 0.85 +0.1 994,873 + Pf6N2E2_849 0.85 +0.9 994,873 + Pf6N2E2_849 0.85 -0.7 994,874 - Pf6N2E2_849 0.85 +1.1 994,874 - Pf6N2E2_849 0.85 +0.1 995,018 + -0.7 995,019 - +0.6 995,029 + -1.0 995,030 - -1.2 995,041 + -0.2 995,125 + -0.9 995,125 + -0.5 995,125 + +0.7 995,125 + +0.0 995,126 - -0.7 995,126 - +0.9 995,126 - -0.2 995,215 + -0.5 995,219 + +1.3 995,219 + -0.2 995,219 + +0.3 995,220 - -3.4 995,225 + +0.6 995,226 - -1.2 995,290 - -1.1 995,297 + -0.2 995,323 - +0.0 995,362 + -0.8 995,739 - Pf6N2E2_851 0.26 +0.6 995,864 - Pf6N2E2_851 0.45 +0.6 995,928 + Pf6N2E2_851 0.55 +0.8 995,929 - Pf6N2E2_851 0.55 +0.6 995,929 - Pf6N2E2_851 0.55 -0.3 996,015 + Pf6N2E2_851 0.69 -1.3 996,015 + Pf6N2E2_851 0.69 -0.7 996,015 + Pf6N2E2_851 0.69 +0.8 996,016 - Pf6N2E2_851 0.69 +1.4 996,016 - Pf6N2E2_851 0.69 -1.9 996,016 - +3.9
Or see this region's nucleotide sequence