Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5916 and Pf6N2E2_5917 are separated by 27 nucleotides
Pf6N2E2_5916 - Flp pilus assembly protein TadB, at 6,734,749 to 6,735,735
_5916
Pf6N2E2_5917 - Type II/IV secretion system protein TadC, associated with Flp pilus assembly, at 6,735,763 to 6,736,728
_5917
Position (kb)
6735
6736
6737 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6734.807 kb on + strand at 6734.808 kb on - strand at 6734.871 kb on + strand, within Pf6N2E2_5916 at 6734.872 kb on - strand, within Pf6N2E2_5916 at 6734.944 kb on + strand, within Pf6N2E2_5916 at 6734.944 kb on + strand, within Pf6N2E2_5916 at 6734.944 kb on + strand, within Pf6N2E2_5916 at 6734.945 kb on - strand, within Pf6N2E2_5916 at 6735.037 kb on + strand, within Pf6N2E2_5916 at 6735.098 kb on + strand, within Pf6N2E2_5916 at 6735.098 kb on + strand, within Pf6N2E2_5916 at 6735.099 kb on - strand, within Pf6N2E2_5916 at 6735.352 kb on + strand, within Pf6N2E2_5916 at 6735.352 kb on + strand, within Pf6N2E2_5916 at 6735.352 kb on + strand, within Pf6N2E2_5916 at 6735.353 kb on - strand, within Pf6N2E2_5916 at 6735.622 kb on + strand, within Pf6N2E2_5916 at 6735.622 kb on + strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.878 kb on + strand, within Pf6N2E2_5917 at 6735.879 kb on - strand, within Pf6N2E2_5917 at 6736.096 kb on + strand, within Pf6N2E2_5917 at 6736.097 kb on - strand, within Pf6N2E2_5917 at 6736.237 kb on + strand at 6736.237 kb on + strand, within Pf6N2E2_5917 at 6736.238 kb on - strand, within Pf6N2E2_5917 at 6736.238 kb on - strand, within Pf6N2E2_5917 at 6736.238 kb on - strand, within Pf6N2E2_5917 at 6736.533 kb on + strand, within Pf6N2E2_5917 at 6736.561 kb on + strand, within Pf6N2E2_5917 at 6736.722 kb on + strand at 6736.722 kb on + strand at 6736.722 kb on + strand at 6736.723 kb on - strand at 6736.723 kb on - strand at 6736.723 kb on - strand at 6736.726 kb on + strand at 6736.726 kb on + strand at 6736.726 kb on + strand at 6736.726 kb on + strand at 6736.726 kb on + strand at 6736.726 kb on + strand at 6736.727 kb on - strand at 6736.727 kb on - strand at 6736.727 kb on - strand at 6736.727 kb on - strand at 6736.727 kb on - strand at 6736.727 kb on - strand at 6736.768 kb on + strand at 6736.768 kb on + strand at 6736.768 kb on + strand at 6736.769 kb on - strand at 6736.769 kb on - strand at 6736.828 kb on + strand at 6736.828 kb on + strand at 6736.828 kb on + strand at 6736.828 kb on + strand at 6736.828 kb on + strand at 6736.828 kb on + strand at 6736.828 kb on + strand at 6736.828 kb on + strand at 6736.828 kb on + strand at 6736.828 kb on + strand at 6736.828 kb on + strand at 6736.828 kb on + strand at 6736.828 kb on + strand at 6736.829 kb on - strand at 6736.829 kb on - strand at 6736.829 kb on - strand at 6736.829 kb on - strand at 6736.829 kb on - strand at 6736.829 kb on - strand at 6736.829 kb on - strand at 6736.832 kb on + strand at 6736.832 kb on + strand at 6736.832 kb on + strand at 6736.833 kb on - strand at 6736.833 kb on - strand at 6736.833 kb on - strand at 6736.904 kb on - strand at 6736.943 kb on + strand at 6737.017 kb on + strand at 6737.028 kb on + strand at 6737.037 kb on + strand at 6737.037 kb on + strand at 6737.037 kb on + strand at 6737.037 kb on + strand at 6737.037 kb on + strand at 6737.038 kb on - strand at 6737.038 kb on - strand at 6737.038 kb on - strand at 6737.083 kb on + strand at 6737.084 kb on - strand at 6737.118 kb on + strand at 6737.119 kb on - strand at 6737.119 kb on - strand at 6737.122 kb on + strand at 6737.122 kb on + strand at 6737.123 kb on - strand at 6737.123 kb on - strand at 6737.158 kb on - strand at 6737.280 kb on + strand at 6737.281 kb on - strand at 6737.281 kb on - strand at 6737.281 kb on - strand at 6737.283 kb on + strand at 6737.308 kb on - strand at 6737.440 kb on + strand at 6737.440 kb on + strand at 6737.440 kb on + strand at 6737.440 kb on + strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.585 kb on + strand at 6737.585 kb on + strand at 6737.585 kb on + strand at 6737.647 kb on - strand at 6737.706 kb on + strand at 6737.707 kb on - strand at 6737.707 kb on - strand at 6737.707 kb on - strand at 6737.707 kb on - strand at 6737.707 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 6,734,807 + -2.2 6,734,808 - -1.7 6,734,871 + Pf6N2E2_5916 0.12 +0.3 6,734,872 - Pf6N2E2_5916 0.12 +0.3 6,734,944 + Pf6N2E2_5916 0.20 +0.0 6,734,944 + Pf6N2E2_5916 0.20 -0.6 6,734,944 + Pf6N2E2_5916 0.20 +1.0 6,734,945 - Pf6N2E2_5916 0.20 +0.1 6,735,037 + Pf6N2E2_5916 0.29 +1.4 6,735,098 + Pf6N2E2_5916 0.35 +0.1 6,735,098 + Pf6N2E2_5916 0.35 -0.2 6,735,099 - Pf6N2E2_5916 0.35 -0.8 6,735,352 + Pf6N2E2_5916 0.61 +0.3 6,735,352 + Pf6N2E2_5916 0.61 -1.0 6,735,352 + Pf6N2E2_5916 0.61 -1.0 6,735,353 - Pf6N2E2_5916 0.61 +1.2 6,735,622 + Pf6N2E2_5916 0.88 -1.5 6,735,622 + Pf6N2E2_5916 0.88 -1.4 6,735,623 - Pf6N2E2_5916 0.89 +0.6 6,735,623 - Pf6N2E2_5916 0.89 -0.9 6,735,623 - Pf6N2E2_5916 0.89 +0.1 6,735,623 - Pf6N2E2_5916 0.89 +0.2 6,735,623 - Pf6N2E2_5916 0.89 +0.7 6,735,878 + Pf6N2E2_5917 0.12 -1.0 6,735,879 - Pf6N2E2_5917 0.12 -0.6 6,736,096 + Pf6N2E2_5917 0.34 +1.1 6,736,097 - Pf6N2E2_5917 0.35 +1.4 6,736,237 + -0.1 6,736,237 + Pf6N2E2_5917 0.49 +2.0 6,736,238 - Pf6N2E2_5917 0.49 +1.0 6,736,238 - Pf6N2E2_5917 0.49 +1.7 6,736,238 - Pf6N2E2_5917 0.49 -2.5 6,736,533 + Pf6N2E2_5917 0.80 -1.0 6,736,561 + Pf6N2E2_5917 0.83 +1.0 6,736,722 + +0.7 6,736,722 + +0.0 6,736,722 + -0.2 6,736,723 - +1.1 6,736,723 - +1.5 6,736,723 - +1.3 6,736,726 + +0.3 6,736,726 + -0.3 6,736,726 + +1.0 6,736,726 + +0.1 6,736,726 + +1.6 6,736,726 + -1.0 6,736,727 - +0.5 6,736,727 - +3.0 6,736,727 - +1.1 6,736,727 - -3.2 6,736,727 - +0.5 6,736,727 - +0.4 6,736,768 + +0.5 6,736,768 + +0.4 6,736,768 + -0.2 6,736,769 - +1.4 6,736,769 - +2.0 6,736,828 + +1.9 6,736,828 + -0.2 6,736,828 + +0.5 6,736,828 + -2.9 6,736,828 + -0.7 6,736,828 + -0.2 6,736,828 + -1.1 6,736,828 + +1.1 6,736,828 + -1.0 6,736,828 + -0.5 6,736,828 + -0.1 6,736,828 + +0.0 6,736,828 + +0.1 6,736,829 - -0.6 6,736,829 - -0.4 6,736,829 - -1.4 6,736,829 - -1.2 6,736,829 - +1.6 6,736,829 - -1.3 6,736,829 - +1.1 6,736,832 + +0.5 6,736,832 + +0.9 6,736,832 + -0.3 6,736,833 - -2.1 6,736,833 - +0.4 6,736,833 - -0.5 6,736,904 - +0.6 6,736,943 + -0.6 6,737,017 + -0.6 6,737,028 + -0.5 6,737,037 + +0.2 6,737,037 + -0.5 6,737,037 + -0.1 6,737,037 + +1.5 6,737,037 + +1.2 6,737,038 - -1.0 6,737,038 - -0.7 6,737,038 - -0.4 6,737,083 + +0.1 6,737,084 - +0.1 6,737,118 + +0.5 6,737,119 - +0.5 6,737,119 - -0.8 6,737,122 + -0.2 6,737,122 + +0.2 6,737,123 - +0.3 6,737,123 - -1.6 6,737,158 - -0.9 6,737,280 + +0.2 6,737,281 - -0.7 6,737,281 - +1.1 6,737,281 - -0.7 6,737,283 + +0.7 6,737,308 - -2.1 6,737,440 + +0.0 6,737,440 + -1.0 6,737,440 + +0.8 6,737,440 + -0.2 6,737,441 - -0.2 6,737,441 - +1.1 6,737,441 - -0.3 6,737,441 - +1.0 6,737,441 - +1.2 6,737,441 - +2.0 6,737,441 - +0.9 6,737,441 - -2.0 6,737,441 - +0.8 6,737,441 - -1.3 6,737,585 + -0.1 6,737,585 + -1.3 6,737,585 + +0.9 6,737,647 - +0.5 6,737,706 + +0.4 6,737,707 - +0.1 6,737,707 - +0.4 6,737,707 - -0.4 6,737,707 - -0.8 6,737,707 - -1.6
Or see this region's nucleotide sequence