Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5777 and Pf6N2E2_5778 are separated by 17 nucleotides Pf6N2E2_5778 and Pf6N2E2_5779 are separated by 470 nucleotides Pf6N2E2_5779 and Pf6N2E2_5780 are separated by 118 nucleotides
Pf6N2E2_5777 - Rhs family protein, at 6,591,062 to 6,595,720
_5777
Pf6N2E2_5778 - hypothetical protein, at 6,595,738 to 6,595,857
_5778
Pf6N2E2_5779 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 6,596,328 to 6,598,115
_5779
Pf6N2E2_5780 - Periplasmic protease, at 6,598,234 to 6,600,345
_5780
Position (kb)
6596
6597
6598
6599 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6595.399 kb on + strand at 6595.399 kb on + strand at 6595.399 kb on + strand at 6595.400 kb on - strand at 6595.400 kb on - strand at 6595.400 kb on - strand at 6595.405 kb on + strand at 6595.405 kb on + strand at 6595.405 kb on + strand at 6595.406 kb on - strand at 6595.450 kb on + strand at 6595.451 kb on - strand at 6595.546 kb on - strand at 6595.550 kb on - strand at 6595.552 kb on + strand at 6595.562 kb on - strand at 6595.573 kb on + strand at 6595.573 kb on + strand at 6595.573 kb on + strand at 6595.574 kb on - strand at 6595.579 kb on + strand at 6595.579 kb on + strand at 6595.612 kb on - strand at 6595.716 kb on + strand at 6595.716 kb on + strand at 6595.716 kb on + strand at 6595.717 kb on - strand at 6595.739 kb on + strand at 6595.851 kb on + strand at 6595.874 kb on + strand at 6595.912 kb on + strand at 6595.916 kb on + strand at 6595.936 kb on + strand at 6595.939 kb on + strand at 6595.939 kb on + strand at 6595.939 kb on + strand at 6595.939 kb on + strand at 6595.945 kb on + strand at 6595.979 kb on + strand at 6595.979 kb on + strand at 6596.007 kb on + strand at 6596.008 kb on - strand at 6596.079 kb on + strand at 6596.079 kb on + strand at 6596.080 kb on - strand at 6596.080 kb on - strand at 6596.104 kb on + strand at 6596.104 kb on + strand at 6596.105 kb on - strand at 6596.198 kb on + strand at 6596.199 kb on - strand at 6596.199 kb on - strand at 6596.199 kb on - strand at 6596.216 kb on - strand at 6596.330 kb on - strand at 6596.330 kb on - strand at 6596.386 kb on + strand at 6596.386 kb on + strand at 6596.387 kb on - strand at 6596.387 kb on - strand at 6596.387 kb on - strand at 6596.387 kb on - strand at 6596.534 kb on + strand, within Pf6N2E2_5779 at 6596.555 kb on + strand, within Pf6N2E2_5779 at 6596.572 kb on - strand, within Pf6N2E2_5779 at 6596.572 kb on - strand, within Pf6N2E2_5779 at 6596.587 kb on + strand, within Pf6N2E2_5779 at 6596.776 kb on + strand, within Pf6N2E2_5779 at 6596.776 kb on + strand, within Pf6N2E2_5779 at 6596.776 kb on + strand, within Pf6N2E2_5779 at 6596.776 kb on + strand, within Pf6N2E2_5779 at 6596.776 kb on + strand, within Pf6N2E2_5779 at 6596.777 kb on - strand, within Pf6N2E2_5779 at 6596.777 kb on - strand, within Pf6N2E2_5779 at 6596.777 kb on - strand, within Pf6N2E2_5779 at 6596.777 kb on - strand, within Pf6N2E2_5779 at 6596.777 kb on - strand, within Pf6N2E2_5779 at 6596.777 kb on - strand, within Pf6N2E2_5779 at 6596.777 kb on - strand, within Pf6N2E2_5779 at 6596.777 kb on - strand, within Pf6N2E2_5779 at 6596.777 kb on - strand, within Pf6N2E2_5779 at 6596.777 kb on - strand, within Pf6N2E2_5779 at 6596.777 kb on - strand, within Pf6N2E2_5779 at 6596.777 kb on - strand, within Pf6N2E2_5779 at 6596.815 kb on + strand, within Pf6N2E2_5779 at 6596.815 kb on + strand, within Pf6N2E2_5779 at 6596.815 kb on + strand, within Pf6N2E2_5779 at 6596.815 kb on + strand, within Pf6N2E2_5779 at 6596.815 kb on + strand, within Pf6N2E2_5779 at 6596.815 kb on + strand, within Pf6N2E2_5779 at 6596.816 kb on - strand, within Pf6N2E2_5779 at 6596.816 kb on - strand, within Pf6N2E2_5779 at 6596.875 kb on - strand, within Pf6N2E2_5779 at 6596.977 kb on + strand, within Pf6N2E2_5779 at 6596.977 kb on + strand, within Pf6N2E2_5779 at 6596.977 kb on + strand, within Pf6N2E2_5779 at 6596.977 kb on + strand, within Pf6N2E2_5779 at 6596.977 kb on + strand, within Pf6N2E2_5779 at 6596.977 kb on + strand, within Pf6N2E2_5779 at 6596.978 kb on - strand, within Pf6N2E2_5779 at 6596.978 kb on - strand, within Pf6N2E2_5779 at 6596.978 kb on - strand, within Pf6N2E2_5779 at 6596.978 kb on - strand, within Pf6N2E2_5779 at 6596.978 kb on - strand, within Pf6N2E2_5779 at 6596.978 kb on - strand, within Pf6N2E2_5779 at 6596.978 kb on - strand, within Pf6N2E2_5779 at 6596.978 kb on - strand, within Pf6N2E2_5779 at 6597.415 kb on + strand, within Pf6N2E2_5779 at 6597.415 kb on + strand, within Pf6N2E2_5779 at 6597.595 kb on + strand, within Pf6N2E2_5779 at 6597.595 kb on + strand, within Pf6N2E2_5779 at 6597.634 kb on + strand, within Pf6N2E2_5779 at 6597.912 kb on + strand, within Pf6N2E2_5779 at 6597.913 kb on - strand, within Pf6N2E2_5779 at 6597.913 kb on - strand, within Pf6N2E2_5779 at 6597.913 kb on - strand, within Pf6N2E2_5779 at 6597.913 kb on - strand, within Pf6N2E2_5779 at 6597.913 kb on - strand, within Pf6N2E2_5779 at 6597.951 kb on + strand at 6597.952 kb on - strand at 6598.007 kb on - strand at 6598.166 kb on + strand at 6598.167 kb on - strand at 6598.167 kb on - strand at 6598.268 kb on + strand at 6598.302 kb on - strand at 6598.856 kb on + strand, within Pf6N2E2_5780 at 6598.856 kb on + strand, within Pf6N2E2_5780 at 6598.934 kb on + strand, within Pf6N2E2_5780 at 6598.934 kb on + strand, within Pf6N2E2_5780 at 6598.934 kb on + strand, within Pf6N2E2_5780 at 6598.935 kb on - strand, within Pf6N2E2_5780 at 6598.935 kb on - strand, within Pf6N2E2_5780 at 6598.935 kb on - strand, within Pf6N2E2_5780
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 6,595,399 + -0.9 6,595,399 + +2.0 6,595,399 + -0.4 6,595,400 - -1.1 6,595,400 - +0.6 6,595,400 - +0.0 6,595,405 + -0.4 6,595,405 + +1.3 6,595,405 + -1.4 6,595,406 - +0.2 6,595,450 + -0.6 6,595,451 - +0.3 6,595,546 - +1.2 6,595,550 - +1.3 6,595,552 + -1.1 6,595,562 - -0.7 6,595,573 + +1.6 6,595,573 + +0.6 6,595,573 + +0.2 6,595,574 - -0.1 6,595,579 + +0.4 6,595,579 + +0.4 6,595,612 - -0.3 6,595,716 + +1.7 6,595,716 + +1.9 6,595,716 + -0.1 6,595,717 - -0.5 6,595,739 + +0.2 6,595,851 + -0.2 6,595,874 + +0.2 6,595,912 + +0.6 6,595,916 + -0.7 6,595,936 + -0.3 6,595,939 + +0.1 6,595,939 + +0.8 6,595,939 + -1.2 6,595,939 + +0.0 6,595,945 + -0.5 6,595,979 + -0.3 6,595,979 + +0.6 6,596,007 + +0.4 6,596,008 - +1.0 6,596,079 + +1.5 6,596,079 + -1.2 6,596,080 - +1.3 6,596,080 - -0.8 6,596,104 + -0.5 6,596,104 + -0.1 6,596,105 - +0.7 6,596,198 + +0.3 6,596,199 - +1.1 6,596,199 - -1.8 6,596,199 - +2.6 6,596,216 - -1.8 6,596,330 - -2.8 6,596,330 - +0.6 6,596,386 + +0.1 6,596,386 + -1.9 6,596,387 - -0.5 6,596,387 - -0.8 6,596,387 - -0.9 6,596,387 - -0.6 6,596,534 + Pf6N2E2_5779 0.12 +0.6 6,596,555 + Pf6N2E2_5779 0.13 -0.5 6,596,572 - Pf6N2E2_5779 0.14 +0.1 6,596,572 - Pf6N2E2_5779 0.14 -0.4 6,596,587 + Pf6N2E2_5779 0.14 +2.0 6,596,776 + Pf6N2E2_5779 0.25 +0.3 6,596,776 + Pf6N2E2_5779 0.25 +3.4 6,596,776 + Pf6N2E2_5779 0.25 +0.6 6,596,776 + Pf6N2E2_5779 0.25 +0.9 6,596,776 + Pf6N2E2_5779 0.25 +1.2 6,596,777 - Pf6N2E2_5779 0.25 +0.3 6,596,777 - Pf6N2E2_5779 0.25 +0.7 6,596,777 - Pf6N2E2_5779 0.25 +0.2 6,596,777 - Pf6N2E2_5779 0.25 -0.4 6,596,777 - Pf6N2E2_5779 0.25 -2.1 6,596,777 - Pf6N2E2_5779 0.25 -2.8 6,596,777 - Pf6N2E2_5779 0.25 +0.1 6,596,777 - Pf6N2E2_5779 0.25 -0.5 6,596,777 - Pf6N2E2_5779 0.25 -0.2 6,596,777 - Pf6N2E2_5779 0.25 -0.2 6,596,777 - Pf6N2E2_5779 0.25 -0.8 6,596,777 - Pf6N2E2_5779 0.25 +0.2 6,596,815 + Pf6N2E2_5779 0.27 +1.3 6,596,815 + Pf6N2E2_5779 0.27 +1.5 6,596,815 + Pf6N2E2_5779 0.27 +0.8 6,596,815 + Pf6N2E2_5779 0.27 -1.1 6,596,815 + Pf6N2E2_5779 0.27 -0.0 6,596,815 + Pf6N2E2_5779 0.27 -0.3 6,596,816 - Pf6N2E2_5779 0.27 +0.5 6,596,816 - Pf6N2E2_5779 0.27 +0.1 6,596,875 - Pf6N2E2_5779 0.31 -1.7 6,596,977 + Pf6N2E2_5779 0.36 +0.2 6,596,977 + Pf6N2E2_5779 0.36 +0.5 6,596,977 + Pf6N2E2_5779 0.36 +2.0 6,596,977 + Pf6N2E2_5779 0.36 +0.0 6,596,977 + Pf6N2E2_5779 0.36 +0.2 6,596,977 + Pf6N2E2_5779 0.36 -0.2 6,596,978 - Pf6N2E2_5779 0.36 +1.3 6,596,978 - Pf6N2E2_5779 0.36 -1.6 6,596,978 - Pf6N2E2_5779 0.36 +0.2 6,596,978 - Pf6N2E2_5779 0.36 -2.5 6,596,978 - Pf6N2E2_5779 0.36 +2.0 6,596,978 - Pf6N2E2_5779 0.36 +0.5 6,596,978 - Pf6N2E2_5779 0.36 +0.0 6,596,978 - Pf6N2E2_5779 0.36 +0.0 6,597,415 + Pf6N2E2_5779 0.61 +1.7 6,597,415 + Pf6N2E2_5779 0.61 +0.6 6,597,595 + Pf6N2E2_5779 0.71 -0.1 6,597,595 + Pf6N2E2_5779 0.71 +1.9 6,597,634 + Pf6N2E2_5779 0.73 -2.3 6,597,912 + Pf6N2E2_5779 0.89 +0.8 6,597,913 - Pf6N2E2_5779 0.89 +0.1 6,597,913 - Pf6N2E2_5779 0.89 +0.3 6,597,913 - Pf6N2E2_5779 0.89 -0.6 6,597,913 - Pf6N2E2_5779 0.89 +0.6 6,597,913 - Pf6N2E2_5779 0.89 +0.9 6,597,951 + +1.4 6,597,952 - -3.4 6,598,007 - -0.7 6,598,166 + +1.0 6,598,167 - +1.6 6,598,167 - +0.3 6,598,268 + -0.7 6,598,302 - -1.0 6,598,856 + Pf6N2E2_5780 0.29 +0.4 6,598,856 + Pf6N2E2_5780 0.29 -0.4 6,598,934 + Pf6N2E2_5780 0.33 +0.7 6,598,934 + Pf6N2E2_5780 0.33 +1.7 6,598,934 + Pf6N2E2_5780 0.33 -2.3 6,598,935 - Pf6N2E2_5780 0.33 -0.6 6,598,935 - Pf6N2E2_5780 0.33 -1.6 6,598,935 - Pf6N2E2_5780 0.33 +0.5
Or see this region's nucleotide sequence