Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5363 and Pf6N2E2_5364 are separated by 373 nucleotides Pf6N2E2_5364 and Pf6N2E2_5365 are separated by 230 nucleotides
Pf6N2E2_5363 - Mobile element protein, at 6,159,860 to 6,161,128
_5363
Pf6N2E2_5364 - GGDEF domain protein, at 6,161,502 to 6,162,995
_5364
Pf6N2E2_5365 - Heavy metal sensor histidine kinase, at 6,163,226 to 6,164,593
_5365
Position (kb)
6161
6162
6163 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 6161.380 kb on + strand at 6161.380 kb on + strand at 6161.384 kb on + strand at 6161.384 kb on + strand at 6161.384 kb on + strand at 6161.384 kb on + strand at 6161.384 kb on + strand at 6161.384 kb on + strand at 6161.384 kb on + strand at 6161.384 kb on + strand at 6161.384 kb on + strand at 6161.384 kb on + strand at 6161.384 kb on + strand at 6161.384 kb on + strand at 6161.384 kb on + strand at 6161.405 kb on + strand at 6161.405 kb on + strand at 6161.433 kb on - strand at 6161.433 kb on - strand at 6161.454 kb on - strand at 6161.549 kb on - strand at 6161.593 kb on + strand at 6161.593 kb on + strand at 6161.687 kb on + strand, within Pf6N2E2_5364 at 6161.688 kb on - strand, within Pf6N2E2_5364 at 6162.048 kb on + strand, within Pf6N2E2_5364 at 6162.059 kb on + strand, within Pf6N2E2_5364 at 6162.060 kb on - strand, within Pf6N2E2_5364 at 6162.060 kb on - strand, within Pf6N2E2_5364 at 6162.145 kb on + strand, within Pf6N2E2_5364 at 6162.146 kb on - strand, within Pf6N2E2_5364 at 6162.146 kb on - strand, within Pf6N2E2_5364 at 6162.199 kb on + strand, within Pf6N2E2_5364 at 6162.199 kb on + strand, within Pf6N2E2_5364 at 6162.200 kb on - strand, within Pf6N2E2_5364 at 6162.249 kb on - strand, within Pf6N2E2_5364 at 6162.267 kb on + strand, within Pf6N2E2_5364 at 6162.267 kb on + strand, within Pf6N2E2_5364 at 6162.267 kb on + strand, within Pf6N2E2_5364 at 6162.409 kb on + strand, within Pf6N2E2_5364 at 6162.409 kb on + strand, within Pf6N2E2_5364 at 6162.410 kb on - strand, within Pf6N2E2_5364 at 6162.410 kb on - strand, within Pf6N2E2_5364 at 6162.410 kb on - strand, within Pf6N2E2_5364 at 6162.473 kb on - strand, within Pf6N2E2_5364 at 6162.586 kb on + strand, within Pf6N2E2_5364 at 6162.586 kb on + strand, within Pf6N2E2_5364 at 6162.586 kb on + strand, within Pf6N2E2_5364 at 6162.586 kb on + strand, within Pf6N2E2_5364 at 6162.587 kb on - strand, within Pf6N2E2_5364 at 6162.587 kb on - strand, within Pf6N2E2_5364 at 6162.587 kb on - strand, within Pf6N2E2_5364 at 6162.627 kb on + strand, within Pf6N2E2_5364 at 6162.627 kb on + strand, within Pf6N2E2_5364 at 6162.628 kb on - strand, within Pf6N2E2_5364 at 6162.628 kb on - strand, within Pf6N2E2_5364 at 6162.667 kb on + strand, within Pf6N2E2_5364 at 6162.667 kb on + strand, within Pf6N2E2_5364 at 6162.778 kb on + strand, within Pf6N2E2_5364 at 6162.778 kb on + strand, within Pf6N2E2_5364 at 6162.936 kb on + strand at 6163.127 kb on - strand at 6163.127 kb on - strand at 6163.506 kb on - strand, within Pf6N2E2_5365 at 6163.776 kb on + strand, within Pf6N2E2_5365 at 6163.776 kb on + strand, within Pf6N2E2_5365 at 6163.776 kb on + strand, within Pf6N2E2_5365 at 6163.776 kb on + strand, within Pf6N2E2_5365 at 6163.776 kb on + strand, within Pf6N2E2_5365 at 6163.776 kb on + strand, within Pf6N2E2_5365 at 6163.777 kb on - strand, within Pf6N2E2_5365 at 6163.777 kb on - strand, within Pf6N2E2_5365 at 6163.890 kb on + strand, within Pf6N2E2_5365 at 6163.890 kb on + strand, within Pf6N2E2_5365 at 6163.890 kb on + strand, within Pf6N2E2_5365
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 6,161,380 + -0.0 6,161,380 + +1.0 6,161,384 + +1.0 6,161,384 + -0.3 6,161,384 + +1.7 6,161,384 + +0.4 6,161,384 + -1.3 6,161,384 + +0.6 6,161,384 + +1.4 6,161,384 + +2.1 6,161,384 + -0.5 6,161,384 + +0.7 6,161,384 + +0.2 6,161,384 + +0.6 6,161,384 + +0.4 6,161,405 + -0.0 6,161,405 + +0.9 6,161,433 - -1.4 6,161,433 - +1.6 6,161,454 - +0.4 6,161,549 - +1.5 6,161,593 + +0.1 6,161,593 + +4.6 6,161,687 + Pf6N2E2_5364 0.12 +1.3 6,161,688 - Pf6N2E2_5364 0.12 +1.3 6,162,048 + Pf6N2E2_5364 0.37 +2.4 6,162,059 + Pf6N2E2_5364 0.37 -0.1 6,162,060 - Pf6N2E2_5364 0.37 +1.1 6,162,060 - Pf6N2E2_5364 0.37 +1.3 6,162,145 + Pf6N2E2_5364 0.43 +1.0 6,162,146 - Pf6N2E2_5364 0.43 -0.3 6,162,146 - Pf6N2E2_5364 0.43 +1.9 6,162,199 + Pf6N2E2_5364 0.47 +0.5 6,162,199 + Pf6N2E2_5364 0.47 +2.7 6,162,200 - Pf6N2E2_5364 0.47 +0.3 6,162,249 - Pf6N2E2_5364 0.50 +1.7 6,162,267 + Pf6N2E2_5364 0.51 +0.0 6,162,267 + Pf6N2E2_5364 0.51 +1.4 6,162,267 + Pf6N2E2_5364 0.51 +1.2 6,162,409 + Pf6N2E2_5364 0.61 +0.6 6,162,409 + Pf6N2E2_5364 0.61 -0.3 6,162,410 - Pf6N2E2_5364 0.61 +1.1 6,162,410 - Pf6N2E2_5364 0.61 -1.6 6,162,410 - Pf6N2E2_5364 0.61 +0.6 6,162,473 - Pf6N2E2_5364 0.65 +1.4 6,162,586 + Pf6N2E2_5364 0.73 +1.1 6,162,586 + Pf6N2E2_5364 0.73 +1.1 6,162,586 + Pf6N2E2_5364 0.73 +0.1 6,162,586 + Pf6N2E2_5364 0.73 -0.3 6,162,587 - Pf6N2E2_5364 0.73 +1.4 6,162,587 - Pf6N2E2_5364 0.73 -1.2 6,162,587 - Pf6N2E2_5364 0.73 +1.1 6,162,627 + Pf6N2E2_5364 0.75 +1.4 6,162,627 + Pf6N2E2_5364 0.75 -0.4 6,162,628 - Pf6N2E2_5364 0.75 +1.4 6,162,628 - Pf6N2E2_5364 0.75 +0.8 6,162,667 + Pf6N2E2_5364 0.78 -0.0 6,162,667 + Pf6N2E2_5364 0.78 +1.1 6,162,778 + Pf6N2E2_5364 0.85 +0.2 6,162,778 + Pf6N2E2_5364 0.85 +0.5 6,162,936 + +0.4 6,163,127 - -2.4 6,163,127 - -2.4 6,163,506 - Pf6N2E2_5365 0.20 -1.5 6,163,776 + Pf6N2E2_5365 0.40 -1.7 6,163,776 + Pf6N2E2_5365 0.40 +0.6 6,163,776 + Pf6N2E2_5365 0.40 +1.6 6,163,776 + Pf6N2E2_5365 0.40 -1.6 6,163,776 + Pf6N2E2_5365 0.40 +1.2 6,163,776 + Pf6N2E2_5365 0.40 -0.8 6,163,777 - Pf6N2E2_5365 0.40 +0.8 6,163,777 - Pf6N2E2_5365 0.40 +0.6 6,163,890 + Pf6N2E2_5365 0.49 +0.6 6,163,890 + Pf6N2E2_5365 0.49 +1.8 6,163,890 + Pf6N2E2_5365 0.49 +0.9
Or see this region's nucleotide sequence