Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5065 and Pf6N2E2_5066 are separated by 78 nucleotides Pf6N2E2_5066 and Pf6N2E2_5067 are separated by 60 nucleotides Pf6N2E2_5067 and Pf6N2E2_5068 are separated by 505 nucleotides
Pf6N2E2_5065 - FIG00960037: hypothetical protein, at 5,840,591 to 5,841,046
_5065
Pf6N2E2_5066 - DJ-1/YajL/PfpI superfamily, includes chaperone protein YajL (former ThiJ), parkinsonism-associated protein DJ-1, peptidases PfpI, Hsp31, at 5,841,125 to 5,841,691
_5066
Pf6N2E2_5067 - MFS general substrate transporter, at 5,841,752 to 5,842,990
_5067
Pf6N2E2_5068 - putative Cytochrome bd2, subunit I, at 5,843,496 to 5,844,932
_5068
Position (kb) 5841
5842
5843 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5840.789 kb on - strand, within Pf6N2E2_5065 at 5840.798 kb on + strand, within Pf6N2E2_5065 at 5840.798 kb on + strand, within Pf6N2E2_5065 at 5840.798 kb on + strand, within Pf6N2E2_5065 at 5840.799 kb on - strand, within Pf6N2E2_5065 at 5840.799 kb on - strand, within Pf6N2E2_5065 at 5840.799 kb on - strand, within Pf6N2E2_5065 at 5840.885 kb on + strand, within Pf6N2E2_5065 at 5840.936 kb on + strand, within Pf6N2E2_5065 at 5840.936 kb on + strand, within Pf6N2E2_5065 at 5840.936 kb on + strand, within Pf6N2E2_5065 at 5840.942 kb on + strand, within Pf6N2E2_5065 at 5840.942 kb on + strand, within Pf6N2E2_5065 at 5840.942 kb on + strand, within Pf6N2E2_5065 at 5840.942 kb on + strand, within Pf6N2E2_5065 at 5840.942 kb on + strand, within Pf6N2E2_5065 at 5840.943 kb on - strand, within Pf6N2E2_5065 at 5840.943 kb on - strand, within Pf6N2E2_5065 at 5841.035 kb on + strand at 5841.036 kb on - strand at 5841.036 kb on - strand at 5841.040 kb on + strand at 5841.040 kb on + strand at 5841.040 kb on + strand at 5841.040 kb on + strand at 5841.040 kb on + strand at 5841.041 kb on - strand at 5841.090 kb on - strand at 5841.090 kb on - strand at 5841.160 kb on + strand at 5841.666 kb on + strand at 5841.666 kb on + strand at 5841.666 kb on + strand at 5841.667 kb on - strand at 5841.681 kb on + strand at 5841.995 kb on + strand, within Pf6N2E2_5067 at 5841.996 kb on - strand, within Pf6N2E2_5067 at 5841.996 kb on - strand, within Pf6N2E2_5067 at 5841.996 kb on - strand, within Pf6N2E2_5067 at 5841.996 kb on - strand, within Pf6N2E2_5067 at 5841.996 kb on - strand, within Pf6N2E2_5067 at 5842.449 kb on + strand, within Pf6N2E2_5067 at 5842.449 kb on + strand, within Pf6N2E2_5067 at 5842.449 kb on + strand, within Pf6N2E2_5067 at 5842.449 kb on + strand, within Pf6N2E2_5067 at 5842.449 kb on + strand at 5842.449 kb on + strand, within Pf6N2E2_5067 at 5842.449 kb on + strand, within Pf6N2E2_5067 at 5842.450 kb on - strand, within Pf6N2E2_5067 at 5842.450 kb on - strand, within Pf6N2E2_5067 at 5842.450 kb on - strand, within Pf6N2E2_5067 at 5842.450 kb on - strand, within Pf6N2E2_5067 at 5842.450 kb on - strand, within Pf6N2E2_5067 at 5842.450 kb on - strand, within Pf6N2E2_5067 at 5842.471 kb on - strand, within Pf6N2E2_5067 at 5842.494 kb on + strand, within Pf6N2E2_5067 at 5842.494 kb on + strand, within Pf6N2E2_5067 at 5842.494 kb on + strand, within Pf6N2E2_5067 at 5842.494 kb on + strand, within Pf6N2E2_5067 at 5842.495 kb on - strand, within Pf6N2E2_5067 at 5842.767 kb on + strand, within Pf6N2E2_5067 at 5842.869 kb on + strand at 5842.869 kb on + strand at 5842.992 kb on + strand at 5842.992 kb on + strand at 5842.992 kb on + strand at 5842.992 kb on + strand at 5842.992 kb on + strand at 5842.993 kb on - strand at 5842.993 kb on - strand at 5842.993 kb on - strand at 5843.024 kb on + strand at 5843.024 kb on + strand at 5843.025 kb on - strand at 5843.025 kb on - strand at 5843.025 kb on - strand at 5843.025 kb on - strand at 5843.048 kb on + strand at 5843.048 kb on + strand at 5843.066 kb on + strand at 5843.067 kb on - strand at 5843.082 kb on + strand at 5843.082 kb on + strand at 5843.083 kb on - strand at 5843.083 kb on - strand at 5843.230 kb on + strand at 5843.230 kb on + strand at 5843.243 kb on + strand at 5843.495 kb on + strand at 5843.495 kb on + strand at 5843.495 kb on + strand at 5843.495 kb on + strand at 5843.495 kb on + strand at 5843.495 kb on + strand at 5843.495 kb on + strand at 5843.495 kb on + strand at 5843.495 kb on + strand at 5843.495 kb on + strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.496 kb on - strand at 5843.596 kb on - strand at 5843.643 kb on + strand, within Pf6N2E2_5068 at 5843.644 kb on - strand, within Pf6N2E2_5068 at 5843.655 kb on + strand, within Pf6N2E2_5068 at 5843.655 kb on + strand, within Pf6N2E2_5068 at 5843.656 kb on - strand, within Pf6N2E2_5068 at 5843.656 kb on - strand, within Pf6N2E2_5068 at 5843.674 kb on + strand, within Pf6N2E2_5068 at 5843.674 kb on + strand, within Pf6N2E2_5068 at 5843.674 kb on + strand, within Pf6N2E2_5068 at 5843.674 kb on + strand, within Pf6N2E2_5068 at 5843.675 kb on - strand, within Pf6N2E2_5068 at 5843.675 kb on - strand, within Pf6N2E2_5068 at 5843.675 kb on - strand, within Pf6N2E2_5068 at 5843.724 kb on + strand, within Pf6N2E2_5068 at 5843.793 kb on + strand, within Pf6N2E2_5068 at 5843.793 kb on + strand, within Pf6N2E2_5068 at 5843.793 kb on + strand, within Pf6N2E2_5068 at 5843.794 kb on - strand, within Pf6N2E2_5068 at 5843.794 kb on - strand, within Pf6N2E2_5068 at 5843.945 kb on + strand, within Pf6N2E2_5068 at 5843.945 kb on + strand, within Pf6N2E2_5068 at 5843.946 kb on - strand, within Pf6N2E2_5068
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,840,789 - Pf6N2E2_5065 0.43 +1.6 5,840,798 + Pf6N2E2_5065 0.45 +0.5 5,840,798 + Pf6N2E2_5065 0.45 -1.2 5,840,798 + Pf6N2E2_5065 0.45 +0.9 5,840,799 - Pf6N2E2_5065 0.46 -0.6 5,840,799 - Pf6N2E2_5065 0.46 -1.0 5,840,799 - Pf6N2E2_5065 0.46 +1.3 5,840,885 + Pf6N2E2_5065 0.64 +1.5 5,840,936 + Pf6N2E2_5065 0.76 -3.0 5,840,936 + Pf6N2E2_5065 0.76 +1.5 5,840,936 + Pf6N2E2_5065 0.76 -0.3 5,840,942 + Pf6N2E2_5065 0.77 -1.5 5,840,942 + Pf6N2E2_5065 0.77 +1.0 5,840,942 + Pf6N2E2_5065 0.77 -2.3 5,840,942 + Pf6N2E2_5065 0.77 +0.3 5,840,942 + Pf6N2E2_5065 0.77 +0.2 5,840,943 - Pf6N2E2_5065 0.77 -0.1 5,840,943 - Pf6N2E2_5065 0.77 +1.0 5,841,035 + +0.1 5,841,036 - -0.1 5,841,036 - +1.1 5,841,040 + -1.9 5,841,040 + +0.7 5,841,040 + +1.2 5,841,040 + -1.5 5,841,040 + -2.5 5,841,041 - -1.8 5,841,090 - -0.5 5,841,090 - +0.8 5,841,160 + +0.1 5,841,666 + -0.0 5,841,666 + -2.7 5,841,666 + +0.2 5,841,667 - -1.3 5,841,681 + -0.7 5,841,995 + Pf6N2E2_5067 0.20 +0.4 5,841,996 - Pf6N2E2_5067 0.20 +0.4 5,841,996 - Pf6N2E2_5067 0.20 +0.6 5,841,996 - Pf6N2E2_5067 0.20 +0.7 5,841,996 - Pf6N2E2_5067 0.20 +1.6 5,841,996 - Pf6N2E2_5067 0.20 -2.6 5,842,449 + Pf6N2E2_5067 0.56 +0.2 5,842,449 + Pf6N2E2_5067 0.56 -0.4 5,842,449 + Pf6N2E2_5067 0.56 -0.1 5,842,449 + Pf6N2E2_5067 0.56 +0.7 5,842,449 + +1.4 5,842,449 + Pf6N2E2_5067 0.56 +0.9 5,842,449 + Pf6N2E2_5067 0.56 +1.5 5,842,450 - Pf6N2E2_5067 0.56 +0.3 5,842,450 - Pf6N2E2_5067 0.56 +1.2 5,842,450 - Pf6N2E2_5067 0.56 -0.1 5,842,450 - Pf6N2E2_5067 0.56 -3.6 5,842,450 - Pf6N2E2_5067 0.56 +2.2 5,842,450 - Pf6N2E2_5067 0.56 +1.8 5,842,471 - Pf6N2E2_5067 0.58 +0.2 5,842,494 + Pf6N2E2_5067 0.60 +0.9 5,842,494 + Pf6N2E2_5067 0.60 +1.0 5,842,494 + Pf6N2E2_5067 0.60 +0.4 5,842,494 + Pf6N2E2_5067 0.60 -1.2 5,842,495 - Pf6N2E2_5067 0.60 +1.6 5,842,767 + Pf6N2E2_5067 0.82 +0.3 5,842,869 + -1.5 5,842,869 + -0.1 5,842,992 + +0.8 5,842,992 + +1.4 5,842,992 + -1.9 5,842,992 + +1.5 5,842,992 + +0.8 5,842,993 - +1.3 5,842,993 - -0.2 5,842,993 - -0.6 5,843,024 + +0.5 5,843,024 + -0.8 5,843,025 - +0.4 5,843,025 - +0.9 5,843,025 - -0.4 5,843,025 - +3.1 5,843,048 + +1.3 5,843,048 + -0.8 5,843,066 + -1.9 5,843,067 - -1.3 5,843,082 + +0.1 5,843,082 + -0.9 5,843,083 - -0.9 5,843,083 - +0.6 5,843,230 + +1.6 5,843,230 + +0.2 5,843,243 + +1.1 5,843,495 + -3.3 5,843,495 + -1.3 5,843,495 + -2.6 5,843,495 + -0.3 5,843,495 + -1.1 5,843,495 + -1.6 5,843,495 + -2.1 5,843,495 + -1.9 5,843,495 + -1.8 5,843,495 + -2.4 5,843,496 - -1.9 5,843,496 - -0.2 5,843,496 - -3.0 5,843,496 - +0.6 5,843,496 - -1.5 5,843,496 - -2.1 5,843,496 - -0.3 5,843,496 - -2.9 5,843,496 - -1.2 5,843,496 - -1.1 5,843,496 - -1.8 5,843,496 - -1.9 5,843,496 - -2.0 5,843,496 - -2.3 5,843,496 - -0.7 5,843,496 - -2.0 5,843,496 - -1.0 5,843,596 - -1.4 5,843,643 + Pf6N2E2_5068 0.10 -3.1 5,843,644 - Pf6N2E2_5068 0.10 -1.8 5,843,655 + Pf6N2E2_5068 0.11 -0.5 5,843,655 + Pf6N2E2_5068 0.11 -3.6 5,843,656 - Pf6N2E2_5068 0.11 -0.9 5,843,656 - Pf6N2E2_5068 0.11 -0.3 5,843,674 + Pf6N2E2_5068 0.12 -0.5 5,843,674 + Pf6N2E2_5068 0.12 -0.9 5,843,674 + Pf6N2E2_5068 0.12 -2.8 5,843,674 + Pf6N2E2_5068 0.12 -2.1 5,843,675 - Pf6N2E2_5068 0.12 -1.5 5,843,675 - Pf6N2E2_5068 0.12 -0.4 5,843,675 - Pf6N2E2_5068 0.12 -2.5 5,843,724 + Pf6N2E2_5068 0.16 -2.2 5,843,793 + Pf6N2E2_5068 0.21 -1.2 5,843,793 + Pf6N2E2_5068 0.21 -2.7 5,843,793 + Pf6N2E2_5068 0.21 -1.2 5,843,794 - Pf6N2E2_5068 0.21 -1.9 5,843,794 - Pf6N2E2_5068 0.21 -0.4 5,843,945 + Pf6N2E2_5068 0.31 +0.1 5,843,945 + Pf6N2E2_5068 0.31 +0.3 5,843,946 - Pf6N2E2_5068 0.31 -2.7
Or see this region's nucleotide sequence