Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3674 and Pf6N2E2_3675 are separated by 11 nucleotides Pf6N2E2_3675 and Pf6N2E2_3676 are separated by 396 nucleotides
Pf6N2E2_3674 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 4,277,874 to 4,280,519
_3674
Pf6N2E2_3675 - Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12), at 4,280,531 to 4,282,168
_3675
Pf6N2E2_3676 - Phosphodiesterase/alkaline phosphatase D-like, at 4,282,565 to 4,283,956
_3676
Position (kb) 4280
4281
4282
4283 Strain fitness (log2 ratio) -1
0
1
2
3 at 4282.167 kb on - strand at 4282.231 kb on + strand at 4282.429 kb on - strand at 4282.429 kb on - strand at 4282.441 kb on - strand at 4282.441 kb on - strand at 4282.478 kb on + strand at 4282.478 kb on + strand at 4282.478 kb on + strand at 4282.479 kb on - strand at 4282.479 kb on - strand at 4282.482 kb on + strand at 4282.500 kb on - strand at 4282.564 kb on + strand at 4282.564 kb on + strand at 4282.565 kb on - strand at 4282.576 kb on + strand at 4282.577 kb on - strand at 4282.577 kb on - strand at 4282.690 kb on + strand at 4282.877 kb on + strand, within Pf6N2E2_3676 at 4282.878 kb on - strand, within Pf6N2E2_3676 at 4282.878 kb on - strand, within Pf6N2E2_3676 at 4282.878 kb on - strand, within Pf6N2E2_3676 at 4282.885 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.924 kb on + strand, within Pf6N2E2_3676 at 4282.924 kb on + strand, within Pf6N2E2_3676 at 4282.924 kb on + strand, within Pf6N2E2_3676 at 4282.925 kb on - strand, within Pf6N2E2_3676 at 4282.925 kb on - strand, within Pf6N2E2_3676 at 4282.925 kb on - strand, within Pf6N2E2_3676 at 4282.925 kb on - strand, within Pf6N2E2_3676 at 4282.944 kb on - strand, within Pf6N2E2_3676 at 4282.974 kb on + strand, within Pf6N2E2_3676 at 4282.975 kb on - strand, within Pf6N2E2_3676 at 4283.072 kb on + strand, within Pf6N2E2_3676 at 4283.073 kb on - strand, within Pf6N2E2_3676 at 4283.073 kb on - strand, within Pf6N2E2_3676 at 4283.073 kb on - strand, within Pf6N2E2_3676 at 4283.073 kb on - strand, within Pf6N2E2_3676
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,282,167 - -0.9 4,282,231 + +0.9 4,282,429 - +0.7 4,282,429 - +0.4 4,282,441 - -1.1 4,282,441 - -0.1 4,282,478 + +1.9 4,282,478 + +0.6 4,282,478 + +1.6 4,282,479 - +0.6 4,282,479 - -0.9 4,282,482 + +0.6 4,282,500 - +0.4 4,282,564 + +0.6 4,282,564 + +0.3 4,282,565 - +0.4 4,282,576 + +1.3 4,282,577 - +1.6 4,282,577 - +2.6 4,282,690 + +1.4 4,282,877 + Pf6N2E2_3676 0.22 -0.6 4,282,878 - Pf6N2E2_3676 0.22 +0.2 4,282,878 - Pf6N2E2_3676 0.22 +0.9 4,282,878 - Pf6N2E2_3676 0.22 +2.1 4,282,885 - Pf6N2E2_3676 0.23 -1.3 4,282,923 - Pf6N2E2_3676 0.26 -0.8 4,282,923 - Pf6N2E2_3676 0.26 +1.3 4,282,923 - Pf6N2E2_3676 0.26 -0.3 4,282,923 - Pf6N2E2_3676 0.26 +1.2 4,282,923 - Pf6N2E2_3676 0.26 -0.6 4,282,923 - Pf6N2E2_3676 0.26 +1.7 4,282,923 - Pf6N2E2_3676 0.26 +0.2 4,282,923 - Pf6N2E2_3676 0.26 +0.2 4,282,923 - Pf6N2E2_3676 0.26 +0.1 4,282,924 + Pf6N2E2_3676 0.26 +3.1 4,282,924 + Pf6N2E2_3676 0.26 -0.3 4,282,924 + Pf6N2E2_3676 0.26 +2.0 4,282,925 - Pf6N2E2_3676 0.26 +0.1 4,282,925 - Pf6N2E2_3676 0.26 +1.1 4,282,925 - Pf6N2E2_3676 0.26 +0.5 4,282,925 - Pf6N2E2_3676 0.26 +0.2 4,282,944 - Pf6N2E2_3676 0.27 +1.9 4,282,974 + Pf6N2E2_3676 0.29 +1.3 4,282,975 - Pf6N2E2_3676 0.29 -0.6 4,283,072 + Pf6N2E2_3676 0.36 +1.4 4,283,073 - Pf6N2E2_3676 0.36 +0.3 4,283,073 - Pf6N2E2_3676 0.36 +0.4 4,283,073 - Pf6N2E2_3676 0.36 -1.0 4,283,073 - Pf6N2E2_3676 0.36 -1.2
Or see this region's nucleotide sequence