Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3514 and Pf6N2E2_3515 are separated by 30 nucleotides Pf6N2E2_3515 and Pf6N2E2_3516 are separated by 13 nucleotides Pf6N2E2_3516 and Pf6N2E2_3517 are separated by 147 nucleotides
Pf6N2E2_3514 - Ribosomal protein L11 methyltransferase (EC 2.1.1.-), at 4,098,951 to 4,099,829
_3514
Pf6N2E2_3515 - Probable transmembrane protein, at 4,099,860 to 4,100,942
_3515
Pf6N2E2_3516 - Probable transmembrane protein, at 4,100,956 to 4,101,090
_3516
Pf6N2E2_3517 - tRNA dihydrouridine synthase B (EC 1.-.-.-), at 4,101,238 to 4,102,245
_3517
Position (kb)
4099
4100
4101 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4098.889 kb on + strand at 4098.889 kb on + strand at 4098.926 kb on + strand at 4098.926 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.968 kb on - strand at 4098.968 kb on - strand at 4098.968 kb on - strand at 4099.002 kb on + strand at 4099.002 kb on + strand at 4099.002 kb on + strand at 4099.003 kb on - strand at 4099.024 kb on + strand at 4099.024 kb on + strand at 4099.025 kb on - strand at 4099.587 kb on + strand, within Pf6N2E2_3514 at 4099.587 kb on + strand, within Pf6N2E2_3514 at 4099.588 kb on - strand, within Pf6N2E2_3514 at 4099.755 kb on + strand at 4099.756 kb on - strand at 4099.827 kb on + strand at 4099.842 kb on + strand at 4100.256 kb on + strand, within Pf6N2E2_3515 at 4100.256 kb on + strand, within Pf6N2E2_3515 at 4100.603 kb on - strand, within Pf6N2E2_3515 at 4100.603 kb on - strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.723 kb on + strand, within Pf6N2E2_3515 at 4100.723 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.923 kb on + strand at 4101.022 kb on + strand, within Pf6N2E2_3516 at 4101.022 kb on + strand, within Pf6N2E2_3516 at 4101.023 kb on - strand, within Pf6N2E2_3516 at 4101.129 kb on + strand at 4101.129 kb on + strand at 4101.130 kb on - strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.262 kb on + strand at 4101.262 kb on + strand at 4101.262 kb on + strand at 4101.262 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.775 kb on + strand, within Pf6N2E2_3517 at 4101.775 kb on + strand, within Pf6N2E2_3517
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,098,889 + -0.6 4,098,889 + -0.7 4,098,926 + -0.6 4,098,926 + -1.4 4,098,967 + +0.0 4,098,967 + -0.7 4,098,967 + +1.4 4,098,967 + +0.3 4,098,967 + -0.5 4,098,967 + -1.0 4,098,967 + -0.6 4,098,967 + +1.5 4,098,967 + -0.8 4,098,968 - +1.1 4,098,968 - -0.1 4,098,968 - -2.8 4,099,002 + +0.7 4,099,002 + -0.1 4,099,002 + -1.6 4,099,003 - +0.1 4,099,024 + -2.3 4,099,024 + -1.1 4,099,025 - -0.7 4,099,587 + Pf6N2E2_3514 0.72 +1.4 4,099,587 + Pf6N2E2_3514 0.72 -0.1 4,099,588 - Pf6N2E2_3514 0.72 -0.6 4,099,755 + +1.4 4,099,756 - +0.1 4,099,827 + -0.7 4,099,842 + +0.5 4,100,256 + Pf6N2E2_3515 0.37 -2.7 4,100,256 + Pf6N2E2_3515 0.37 -2.0 4,100,603 - Pf6N2E2_3515 0.69 -2.1 4,100,603 - Pf6N2E2_3515 0.69 -1.3 4,100,635 + Pf6N2E2_3515 0.72 -1.3 4,100,635 + Pf6N2E2_3515 0.72 -1.1 4,100,635 + Pf6N2E2_3515 0.72 -1.9 4,100,635 + Pf6N2E2_3515 0.72 +0.9 4,100,636 - Pf6N2E2_3515 0.72 -0.7 4,100,636 - Pf6N2E2_3515 0.72 -1.8 4,100,636 - Pf6N2E2_3515 0.72 -1.7 4,100,636 - Pf6N2E2_3515 0.72 -0.4 4,100,723 + Pf6N2E2_3515 0.80 -3.5 4,100,723 + Pf6N2E2_3515 0.80 -1.3 4,100,821 + Pf6N2E2_3515 0.89 -0.8 4,100,821 + Pf6N2E2_3515 0.89 -3.3 4,100,821 + Pf6N2E2_3515 0.89 -1.0 4,100,821 + Pf6N2E2_3515 0.89 -0.9 4,100,822 - Pf6N2E2_3515 0.89 -0.8 4,100,822 - Pf6N2E2_3515 0.89 -0.9 4,100,822 - Pf6N2E2_3515 0.89 -0.6 4,100,822 - Pf6N2E2_3515 0.89 -1.0 4,100,822 - Pf6N2E2_3515 0.89 -3.1 4,100,923 + -0.7 4,101,022 + Pf6N2E2_3516 0.49 -1.5 4,101,022 + Pf6N2E2_3516 0.49 -1.9 4,101,023 - Pf6N2E2_3516 0.50 -2.5 4,101,129 + +1.3 4,101,129 + +0.0 4,101,130 - -0.8 4,101,237 + +1.5 4,101,237 + -0.6 4,101,237 + +0.6 4,101,237 + +0.6 4,101,237 + -0.2 4,101,262 + +0.8 4,101,262 + -0.8 4,101,262 + +1.1 4,101,262 + -0.3 4,101,264 + +0.4 4,101,264 + +0.9 4,101,264 + +0.6 4,101,264 + +1.5 4,101,264 + +0.9 4,101,264 + +0.8 4,101,264 + +0.3 4,101,264 + +0.2 4,101,264 + -1.2 4,101,264 + +0.7 4,101,264 + -0.8 4,101,264 + +0.5 4,101,264 + +1.0 4,101,264 + +1.0 4,101,264 + +0.2 4,101,264 + +0.4 4,101,264 + -1.3 4,101,264 + -0.7 4,101,775 + Pf6N2E2_3517 0.53 +0.9 4,101,775 + Pf6N2E2_3517 0.53 +1.7
Or see this region's nucleotide sequence