Experiment: inner cut, LB soft agar motility assay 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf6N2E2_3128 and Pf6N2E2_3129 are separated by 1 nucleotides Pf6N2E2_3129 and Pf6N2E2_3130 are separated by 25 nucleotides Pf6N2E2_3130 and Pf6N2E2_3131 are separated by 72 nucleotides  
        
        Pf6N2E2_3128 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 3,619,634 to 3,620,671 
         
        _3128 
         
        
        Pf6N2E2_3129 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 3,620,673 to 3,621,620 
         
        _3129 
         
        
        Pf6N2E2_3130 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 3,621,646 to 3,622,428 
         
        _3130 
         
        
        Pf6N2E2_3131 - 4-aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 3,622,501 to 3,623,925 
         
        _3131 
         Position (kb)  
3620 
 
3621 
 
3622 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 at 3619.853 kb on + strand, within Pf6N2E2_3128 at 3619.871 kb on - strand, within Pf6N2E2_3128 at 3619.871 kb on - strand, within Pf6N2E2_3128 at 3619.904 kb on - strand, within Pf6N2E2_3128 at 3619.904 kb on - strand, within Pf6N2E2_3128 at 3620.300 kb on + strand, within Pf6N2E2_3128 at 3620.300 kb on + strand, within Pf6N2E2_3128 at 3620.301 kb on - strand, within Pf6N2E2_3128 at 3620.301 kb on - strand, within Pf6N2E2_3128 at 3620.301 kb on - strand, within Pf6N2E2_3128 at 3620.301 kb on - strand, within Pf6N2E2_3128 at 3620.489 kb on + strand, within Pf6N2E2_3128 at 3620.510 kb on + strand, within Pf6N2E2_3128 at 3620.760 kb on + strand at 3620.761 kb on - strand at 3620.761 kb on - strand at 3620.761 kb on - strand at 3620.808 kb on + strand, within Pf6N2E2_3129 at 3620.809 kb on - strand, within Pf6N2E2_3129 at 3620.847 kb on + strand, within Pf6N2E2_3129 at 3620.848 kb on - strand, within Pf6N2E2_3129 at 3621.006 kb on + strand, within Pf6N2E2_3129 at 3621.006 kb on + strand, within Pf6N2E2_3129 at 3621.006 kb on + strand, within Pf6N2E2_3129 at 3621.007 kb on - strand, within Pf6N2E2_3129 at 3621.007 kb on - strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.022 kb on - strand, within Pf6N2E2_3129 at 3621.051 kb on + strand, within Pf6N2E2_3129 at 3621.051 kb on + strand, within Pf6N2E2_3129 at 3621.052 kb on - strand, within Pf6N2E2_3129 at 3621.084 kb on + strand, within Pf6N2E2_3129 at 3621.084 kb on + strand, within Pf6N2E2_3129 at 3621.085 kb on - strand, within Pf6N2E2_3129 at 3621.085 kb on - strand, within Pf6N2E2_3129 at 3621.085 kb on - strand, within Pf6N2E2_3129 at 3621.085 kb on - strand, within Pf6N2E2_3129 at 3621.099 kb on + strand, within Pf6N2E2_3129 at 3621.100 kb on - strand, within Pf6N2E2_3129 at 3621.100 kb on - strand, within Pf6N2E2_3129 at 3621.100 kb on - strand, within Pf6N2E2_3129 at 3621.100 kb on - strand, within Pf6N2E2_3129 at 3621.255 kb on + strand, within Pf6N2E2_3129 at 3621.256 kb on - strand, within Pf6N2E2_3129 at 3621.256 kb on - strand, within Pf6N2E2_3129 at 3621.477 kb on + strand, within Pf6N2E2_3129 at 3621.477 kb on + strand, within Pf6N2E2_3129 at 3621.478 kb on - strand, within Pf6N2E2_3129 at 3621.478 kb on - strand, within Pf6N2E2_3129 at 3621.499 kb on - strand, within Pf6N2E2_3129 at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.577 kb on - strand at 3621.577 kb on - strand at 3621.577 kb on - strand at 3621.580 kb on + strand at 3621.580 kb on + strand at 3621.580 kb on + strand at 3621.581 kb on - strand at 3621.581 kb on - strand at 3621.709 kb on + strand at 3621.919 kb on - strand, within Pf6N2E2_3130 at 3622.063 kb on + strand, within Pf6N2E2_3130 at 3622.157 kb on - strand, within Pf6N2E2_3130 at 3622.349 kb on + strand, within Pf6N2E2_3130 at 3622.349 kb on + strand, within Pf6N2E2_3130 at 3622.350 kb on - strand at 3622.378 kb on + strand at 3622.378 kb on + strand at 3622.379 kb on - strand at 3622.379 kb on - strand at 3622.381 kb on + strand at 3622.381 kb on + strand at 3622.381 kb on + strand at 3622.426 kb on + strand at 3622.426 kb on + strand at 3622.426 kb on + strand at 3622.427 kb on - strand at 3622.427 kb on - strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   inner cut, LB soft agar motility assay             remove   3,619,853   +    Pf6N2E2_3128   0.21  +1.3   3,619,871   -    Pf6N2E2_3128   0.23  +0.1   3,619,871   -    Pf6N2E2_3128   0.23  +0.7   3,619,904   -    Pf6N2E2_3128   0.26  +0.2   3,619,904   -    Pf6N2E2_3128   0.26  +0.7   3,620,300   +    Pf6N2E2_3128   0.64  -2.8   3,620,300   +    Pf6N2E2_3128   0.64  +0.8   3,620,301   -    Pf6N2E2_3128   0.64  -0.4   3,620,301   -    Pf6N2E2_3128   0.64  +0.1   3,620,301   -    Pf6N2E2_3128   0.64  -2.9   3,620,301   -    Pf6N2E2_3128   0.64  -1.0   3,620,489   +    Pf6N2E2_3128   0.82  -1.0   3,620,510   +    Pf6N2E2_3128   0.84  +0.2   3,620,760   +        -0.3   3,620,761   -        -0.2   3,620,761   -        -0.2   3,620,761   -        -0.5   3,620,808   +    Pf6N2E2_3129   0.14  -0.3   3,620,809   -    Pf6N2E2_3129   0.14  -1.1   3,620,847   +    Pf6N2E2_3129   0.18  -1.2   3,620,848   -    Pf6N2E2_3129   0.18  +2.2   3,621,006   +    Pf6N2E2_3129   0.35  +0.8   3,621,006   +    Pf6N2E2_3129   0.35  -1.5   3,621,006   +    Pf6N2E2_3129   0.35  +0.5   3,621,007   -    Pf6N2E2_3129   0.35  -0.5   3,621,007   -    Pf6N2E2_3129   0.35  -0.7   3,621,009   +    Pf6N2E2_3129   0.35  -1.4   3,621,009   +    Pf6N2E2_3129   0.35  -0.7   3,621,009   +    Pf6N2E2_3129   0.35  -1.5   3,621,009   +    Pf6N2E2_3129   0.35  +0.5   3,621,009   +    Pf6N2E2_3129   0.35  -0.8   3,621,009   +    Pf6N2E2_3129   0.35  -0.9   3,621,009   +    Pf6N2E2_3129   0.35  +0.2   3,621,009   +    Pf6N2E2_3129   0.35  +0.9   3,621,009   +    Pf6N2E2_3129   0.35  -1.0   3,621,010   -    Pf6N2E2_3129   0.36  +0.4   3,621,010   -    Pf6N2E2_3129   0.36  -1.8   3,621,010   -    Pf6N2E2_3129   0.36  +0.4   3,621,010   -    Pf6N2E2_3129   0.36  -1.0   3,621,010   -    Pf6N2E2_3129   0.36  +0.0   3,621,010   -    Pf6N2E2_3129   0.36  -0.7   3,621,010   -    Pf6N2E2_3129   0.36  -0.6   3,621,010   -    Pf6N2E2_3129   0.36  -1.3   3,621,010   -    Pf6N2E2_3129   0.36  -0.6   3,621,010   -    Pf6N2E2_3129   0.36  -0.5   3,621,022   -    Pf6N2E2_3129   0.37  -1.3   3,621,051   +    Pf6N2E2_3129   0.40  -1.6   3,621,051   +    Pf6N2E2_3129   0.40  +0.4   3,621,052   -    Pf6N2E2_3129   0.40  +0.3   3,621,084   +    Pf6N2E2_3129   0.43  -0.8   3,621,084   +    Pf6N2E2_3129   0.43  -0.3   3,621,085   -    Pf6N2E2_3129   0.43  +0.4   3,621,085   -    Pf6N2E2_3129   0.43  +0.4   3,621,085   -    Pf6N2E2_3129   0.43  +1.4   3,621,085   -    Pf6N2E2_3129   0.43  -0.2   3,621,099   +    Pf6N2E2_3129   0.45  -0.7   3,621,100   -    Pf6N2E2_3129   0.45  -0.5   3,621,100   -    Pf6N2E2_3129   0.45  +0.3   3,621,100   -    Pf6N2E2_3129   0.45  +0.4   3,621,100   -    Pf6N2E2_3129   0.45  -1.8   3,621,255   +    Pf6N2E2_3129   0.61  -1.2   3,621,256   -    Pf6N2E2_3129   0.61  -0.9   3,621,256   -    Pf6N2E2_3129   0.61  +0.2   3,621,477   +    Pf6N2E2_3129   0.85  -0.1   3,621,477   +    Pf6N2E2_3129   0.85  +0.6   3,621,478   -    Pf6N2E2_3129   0.85  -0.4   3,621,478   -    Pf6N2E2_3129   0.85  +0.6   3,621,499   -    Pf6N2E2_3129   0.87  +0.8   3,621,576   +        +1.1   3,621,576   +        -0.0   3,621,576   +        -1.9   3,621,576   +        -2.3   3,621,576   +        -0.3   3,621,577   -        +0.0   3,621,577   -        -3.5   3,621,577   -        -1.1   3,621,580   +        +1.3   3,621,580   +        -0.1   3,621,580   +        +0.9   3,621,581   -        -0.2   3,621,581   -        -1.6   3,621,709   +        -1.1   3,621,919   -    Pf6N2E2_3130   0.35  -1.9   3,622,063   +    Pf6N2E2_3130   0.53  -2.6   3,622,157   -    Pf6N2E2_3130   0.65  -1.9   3,622,349   +    Pf6N2E2_3130   0.90  +0.4   3,622,349   +    Pf6N2E2_3130   0.90  +0.4   3,622,350   -        +0.2   3,622,378   +        -1.8   3,622,378   +        -0.9   3,622,379   -        +1.3   3,622,379   -        +0.4   3,622,381   +        -1.6   3,622,381   +        +0.6   3,622,381   +        -1.4   3,622,426   +        +1.4   3,622,426   +        -0.1   3,622,426   +        +0.9   3,622,427   -        -0.3   3,622,427   -        -0.5 
 
Or see this region's nucleotide sequence