Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2612 and Pf6N2E2_2613 are separated by 66 nucleotides Pf6N2E2_2613 and Pf6N2E2_2614 are separated by 15 nucleotides Pf6N2E2_2614 and Pf6N2E2_2615 overlap by 11 nucleotides Pf6N2E2_2615 and Pf6N2E2_2616 overlap by 4 nucleotides
Pf6N2E2_2612 - O-antigen biosynthesis protein, at 3,068,784 to 3,071,702
_2612
Pf6N2E2_2613 - Cephalosporin hydroxylase, at 3,071,769 to 3,072,506
_2613
Pf6N2E2_2614 - C-methyltransferase, at 3,072,522 to 3,073,592
_2614
Pf6N2E2_2615 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 3,073,582 to 3,074,439
_2615
Pf6N2E2_2616 - methyltransferase, putative, at 3,074,436 to 3,075,662
_2616
Position (kb)
3072
3073
3074 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3071.605 kb on + strand at 3071.605 kb on + strand at 3071.606 kb on - strand at 3071.606 kb on - strand at 3071.609 kb on - strand at 3071.609 kb on - strand at 3071.609 kb on - strand at 3071.619 kb on - strand at 3071.619 kb on - strand at 3071.702 kb on + strand at 3071.702 kb on + strand at 3071.702 kb on + strand at 3071.703 kb on - strand at 3071.717 kb on + strand at 3071.717 kb on + strand at 3071.718 kb on - strand at 3071.724 kb on + strand at 3071.725 kb on - strand at 3071.725 kb on - strand at 3071.866 kb on + strand, within Pf6N2E2_2613 at 3071.866 kb on + strand, within Pf6N2E2_2613 at 3071.867 kb on - strand, within Pf6N2E2_2613 at 3071.968 kb on + strand, within Pf6N2E2_2613 at 3071.968 kb on + strand, within Pf6N2E2_2613 at 3071.969 kb on - strand, within Pf6N2E2_2613 at 3071.969 kb on - strand, within Pf6N2E2_2613 at 3071.969 kb on - strand, within Pf6N2E2_2613 at 3071.995 kb on + strand, within Pf6N2E2_2613 at 3072.238 kb on + strand, within Pf6N2E2_2613 at 3072.241 kb on + strand, within Pf6N2E2_2613 at 3072.241 kb on + strand, within Pf6N2E2_2613 at 3072.241 kb on + strand, within Pf6N2E2_2613 at 3072.241 kb on + strand, within Pf6N2E2_2613 at 3072.242 kb on - strand, within Pf6N2E2_2613 at 3072.242 kb on - strand, within Pf6N2E2_2613 at 3072.242 kb on - strand, within Pf6N2E2_2613 at 3072.242 kb on - strand, within Pf6N2E2_2613 at 3072.373 kb on + strand, within Pf6N2E2_2613 at 3072.374 kb on - strand, within Pf6N2E2_2613 at 3072.379 kb on + strand, within Pf6N2E2_2613 at 3072.379 kb on + strand, within Pf6N2E2_2613 at 3072.379 kb on + strand, within Pf6N2E2_2613 at 3072.379 kb on + strand, within Pf6N2E2_2613 at 3072.379 kb on + strand, within Pf6N2E2_2613 at 3072.379 kb on + strand, within Pf6N2E2_2613 at 3072.379 kb on + strand, within Pf6N2E2_2613 at 3072.379 kb on + strand, within Pf6N2E2_2613 at 3072.379 kb on + strand, within Pf6N2E2_2613 at 3072.380 kb on - strand, within Pf6N2E2_2613 at 3072.380 kb on - strand, within Pf6N2E2_2613 at 3072.380 kb on - strand, within Pf6N2E2_2613 at 3072.380 kb on - strand, within Pf6N2E2_2613 at 3072.380 kb on - strand, within Pf6N2E2_2613 at 3072.380 kb on - strand, within Pf6N2E2_2613 at 3072.380 kb on - strand, within Pf6N2E2_2613 at 3072.380 kb on - strand, within Pf6N2E2_2613 at 3072.380 kb on - strand, within Pf6N2E2_2613 at 3072.383 kb on + strand, within Pf6N2E2_2613 at 3072.383 kb on + strand, within Pf6N2E2_2613 at 3072.383 kb on + strand, within Pf6N2E2_2613 at 3072.384 kb on - strand, within Pf6N2E2_2613 at 3072.384 kb on - strand, within Pf6N2E2_2613 at 3072.485 kb on + strand at 3072.485 kb on + strand at 3072.559 kb on + strand at 3072.560 kb on - strand at 3072.610 kb on - strand at 3072.610 kb on - strand at 3072.615 kb on + strand at 3072.615 kb on + strand at 3072.615 kb on + strand at 3072.757 kb on - strand, within Pf6N2E2_2614 at 3072.757 kb on - strand, within Pf6N2E2_2614 at 3072.757 kb on - strand, within Pf6N2E2_2614 at 3072.763 kb on + strand, within Pf6N2E2_2614 at 3072.764 kb on - strand, within Pf6N2E2_2614 at 3072.931 kb on + strand, within Pf6N2E2_2614 at 3072.931 kb on + strand, within Pf6N2E2_2614 at 3072.931 kb on + strand, within Pf6N2E2_2614 at 3072.931 kb on + strand, within Pf6N2E2_2614 at 3072.932 kb on - strand, within Pf6N2E2_2614 at 3072.932 kb on - strand, within Pf6N2E2_2614 at 3072.932 kb on - strand, within Pf6N2E2_2614 at 3072.932 kb on - strand, within Pf6N2E2_2614 at 3072.938 kb on - strand, within Pf6N2E2_2614 at 3072.970 kb on - strand, within Pf6N2E2_2614 at 3073.009 kb on + strand, within Pf6N2E2_2614 at 3073.009 kb on + strand, within Pf6N2E2_2614 at 3073.024 kb on + strand, within Pf6N2E2_2614 at 3073.024 kb on + strand, within Pf6N2E2_2614 at 3073.085 kb on - strand, within Pf6N2E2_2614 at 3073.146 kb on - strand, within Pf6N2E2_2614 at 3073.240 kb on + strand, within Pf6N2E2_2614 at 3073.240 kb on + strand, within Pf6N2E2_2614 at 3073.267 kb on + strand, within Pf6N2E2_2614 at 3073.298 kb on - strand, within Pf6N2E2_2614 at 3073.415 kb on - strand, within Pf6N2E2_2614 at 3073.426 kb on + strand, within Pf6N2E2_2614 at 3073.426 kb on + strand, within Pf6N2E2_2614 at 3073.427 kb on - strand, within Pf6N2E2_2614 at 3073.427 kb on - strand, within Pf6N2E2_2614 at 3073.427 kb on - strand, within Pf6N2E2_2614 at 3073.427 kb on - strand, within Pf6N2E2_2614 at 3073.542 kb on - strand at 3073.745 kb on - strand, within Pf6N2E2_2615 at 3073.745 kb on - strand, within Pf6N2E2_2615 at 3073.805 kb on + strand, within Pf6N2E2_2615 at 3073.805 kb on + strand, within Pf6N2E2_2615 at 3073.806 kb on - strand, within Pf6N2E2_2615 at 3073.806 kb on - strand, within Pf6N2E2_2615 at 3073.832 kb on + strand, within Pf6N2E2_2615 at 3073.943 kb on + strand, within Pf6N2E2_2615 at 3073.943 kb on + strand, within Pf6N2E2_2615 at 3073.944 kb on - strand, within Pf6N2E2_2615 at 3074.013 kb on + strand, within Pf6N2E2_2615 at 3074.173 kb on - strand, within Pf6N2E2_2615 at 3074.173 kb on - strand, within Pf6N2E2_2615 at 3074.173 kb on - strand, within Pf6N2E2_2615 at 3074.173 kb on - strand, within Pf6N2E2_2615 at 3074.199 kb on - strand, within Pf6N2E2_2615 at 3074.358 kb on + strand at 3074.428 kb on + strand at 3074.429 kb on - strand at 3074.439 kb on + strand at 3074.439 kb on + strand at 3074.548 kb on + strand at 3074.548 kb on + strand at 3074.548 kb on + strand at 3074.549 kb on - strand at 3074.549 kb on - strand at 3074.549 kb on - strand at 3074.563 kb on - strand, within Pf6N2E2_2616 at 3074.570 kb on + strand, within Pf6N2E2_2616 at 3074.588 kb on + strand, within Pf6N2E2_2616 at 3074.588 kb on + strand, within Pf6N2E2_2616 at 3074.588 kb on + strand, within Pf6N2E2_2616 at 3074.588 kb on + strand, within Pf6N2E2_2616 at 3074.589 kb on - strand, within Pf6N2E2_2616 at 3074.589 kb on - strand, within Pf6N2E2_2616 at 3074.589 kb on - strand, within Pf6N2E2_2616 at 3074.589 kb on - strand, within Pf6N2E2_2616 at 3074.589 kb on - strand, within Pf6N2E2_2616
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,071,605 + +1.8 3,071,605 + +2.1 3,071,606 - +2.2 3,071,606 - +1.5 3,071,609 - +0.0 3,071,609 - +0.8 3,071,609 - +1.2 3,071,619 - +1.9 3,071,619 - +2.2 3,071,702 + +0.2 3,071,702 + +1.9 3,071,702 + +0.8 3,071,703 - +0.7 3,071,717 + +0.3 3,071,717 + +0.1 3,071,718 - +0.2 3,071,724 + +1.0 3,071,725 - +0.1 3,071,725 - +0.6 3,071,866 + Pf6N2E2_2613 0.13 -0.1 3,071,866 + Pf6N2E2_2613 0.13 +1.7 3,071,867 - Pf6N2E2_2613 0.13 -0.4 3,071,968 + Pf6N2E2_2613 0.27 +0.4 3,071,968 + Pf6N2E2_2613 0.27 +0.9 3,071,969 - Pf6N2E2_2613 0.27 +1.3 3,071,969 - Pf6N2E2_2613 0.27 -0.1 3,071,969 - Pf6N2E2_2613 0.27 +0.4 3,071,995 + Pf6N2E2_2613 0.31 -1.6 3,072,238 + Pf6N2E2_2613 0.64 +0.9 3,072,241 + Pf6N2E2_2613 0.64 -0.7 3,072,241 + Pf6N2E2_2613 0.64 +0.1 3,072,241 + Pf6N2E2_2613 0.64 -2.3 3,072,241 + Pf6N2E2_2613 0.64 +1.7 3,072,242 - Pf6N2E2_2613 0.64 -0.4 3,072,242 - Pf6N2E2_2613 0.64 +1.4 3,072,242 - Pf6N2E2_2613 0.64 +1.7 3,072,242 - Pf6N2E2_2613 0.64 +0.3 3,072,373 + Pf6N2E2_2613 0.82 +2.1 3,072,374 - Pf6N2E2_2613 0.82 +0.9 3,072,379 + Pf6N2E2_2613 0.83 -1.9 3,072,379 + Pf6N2E2_2613 0.83 +2.3 3,072,379 + Pf6N2E2_2613 0.83 -1.5 3,072,379 + Pf6N2E2_2613 0.83 +1.8 3,072,379 + Pf6N2E2_2613 0.83 +2.5 3,072,379 + Pf6N2E2_2613 0.83 +1.2 3,072,379 + Pf6N2E2_2613 0.83 +0.1 3,072,379 + Pf6N2E2_2613 0.83 -0.4 3,072,379 + Pf6N2E2_2613 0.83 +1.4 3,072,380 - Pf6N2E2_2613 0.83 -0.3 3,072,380 - Pf6N2E2_2613 0.83 -1.4 3,072,380 - Pf6N2E2_2613 0.83 -0.9 3,072,380 - Pf6N2E2_2613 0.83 -1.1 3,072,380 - Pf6N2E2_2613 0.83 -0.3 3,072,380 - Pf6N2E2_2613 0.83 +0.9 3,072,380 - Pf6N2E2_2613 0.83 -0.1 3,072,380 - Pf6N2E2_2613 0.83 -0.3 3,072,380 - Pf6N2E2_2613 0.83 +1.3 3,072,383 + Pf6N2E2_2613 0.83 +1.0 3,072,383 + Pf6N2E2_2613 0.83 +0.4 3,072,383 + Pf6N2E2_2613 0.83 -0.4 3,072,384 - Pf6N2E2_2613 0.83 +0.9 3,072,384 - Pf6N2E2_2613 0.83 +0.5 3,072,485 + +1.0 3,072,485 + +2.9 3,072,559 + +1.2 3,072,560 - -1.0 3,072,610 - +1.2 3,072,610 - -0.2 3,072,615 + -2.3 3,072,615 + -1.5 3,072,615 + -1.1 3,072,757 - Pf6N2E2_2614 0.22 -0.8 3,072,757 - Pf6N2E2_2614 0.22 +0.9 3,072,757 - Pf6N2E2_2614 0.22 +0.4 3,072,763 + Pf6N2E2_2614 0.23 +0.5 3,072,764 - Pf6N2E2_2614 0.23 -0.2 3,072,931 + Pf6N2E2_2614 0.38 +1.4 3,072,931 + Pf6N2E2_2614 0.38 -0.6 3,072,931 + Pf6N2E2_2614 0.38 -1.3 3,072,931 + Pf6N2E2_2614 0.38 -2.2 3,072,932 - Pf6N2E2_2614 0.38 +1.1 3,072,932 - Pf6N2E2_2614 0.38 +1.5 3,072,932 - Pf6N2E2_2614 0.38 +0.3 3,072,932 - Pf6N2E2_2614 0.38 +1.3 3,072,938 - Pf6N2E2_2614 0.39 -0.1 3,072,970 - Pf6N2E2_2614 0.42 -0.9 3,073,009 + Pf6N2E2_2614 0.45 -1.1 3,073,009 + Pf6N2E2_2614 0.45 +1.7 3,073,024 + Pf6N2E2_2614 0.47 -0.6 3,073,024 + Pf6N2E2_2614 0.47 +1.1 3,073,085 - Pf6N2E2_2614 0.53 -0.2 3,073,146 - Pf6N2E2_2614 0.58 +0.2 3,073,240 + Pf6N2E2_2614 0.67 +0.5 3,073,240 + Pf6N2E2_2614 0.67 +2.9 3,073,267 + Pf6N2E2_2614 0.70 -1.2 3,073,298 - Pf6N2E2_2614 0.72 +0.5 3,073,415 - Pf6N2E2_2614 0.83 +1.5 3,073,426 + Pf6N2E2_2614 0.84 -0.2 3,073,426 + Pf6N2E2_2614 0.84 -1.3 3,073,427 - Pf6N2E2_2614 0.85 -0.9 3,073,427 - Pf6N2E2_2614 0.85 -0.3 3,073,427 - Pf6N2E2_2614 0.85 +0.5 3,073,427 - Pf6N2E2_2614 0.85 +2.0 3,073,542 - -0.2 3,073,745 - Pf6N2E2_2615 0.19 +0.9 3,073,745 - Pf6N2E2_2615 0.19 -0.6 3,073,805 + Pf6N2E2_2615 0.26 +1.1 3,073,805 + Pf6N2E2_2615 0.26 -0.2 3,073,806 - Pf6N2E2_2615 0.26 +1.0 3,073,806 - Pf6N2E2_2615 0.26 +0.3 3,073,832 + Pf6N2E2_2615 0.29 +1.1 3,073,943 + Pf6N2E2_2615 0.42 -1.0 3,073,943 + Pf6N2E2_2615 0.42 -0.2 3,073,944 - Pf6N2E2_2615 0.42 +1.5 3,074,013 + Pf6N2E2_2615 0.50 -0.5 3,074,173 - Pf6N2E2_2615 0.69 -0.6 3,074,173 - Pf6N2E2_2615 0.69 +0.5 3,074,173 - Pf6N2E2_2615 0.69 -0.6 3,074,173 - Pf6N2E2_2615 0.69 +0.8 3,074,199 - Pf6N2E2_2615 0.72 +0.5 3,074,358 + -0.5 3,074,428 + -0.2 3,074,429 - +0.2 3,074,439 + -1.9 3,074,439 + +1.8 3,074,548 + +1.1 3,074,548 + +1.0 3,074,548 + +0.1 3,074,549 - +1.9 3,074,549 - -1.6 3,074,549 - -0.5 3,074,563 - Pf6N2E2_2616 0.10 +2.0 3,074,570 + Pf6N2E2_2616 0.11 -0.4 3,074,588 + Pf6N2E2_2616 0.12 +0.5 3,074,588 + Pf6N2E2_2616 0.12 -0.6 3,074,588 + Pf6N2E2_2616 0.12 -0.6 3,074,588 + Pf6N2E2_2616 0.12 -0.1 3,074,589 - Pf6N2E2_2616 0.12 +1.8 3,074,589 - Pf6N2E2_2616 0.12 +0.5 3,074,589 - Pf6N2E2_2616 0.12 +0.9 3,074,589 - Pf6N2E2_2616 0.12 -0.5 3,074,589 - Pf6N2E2_2616 0.12 -0.6
Or see this region's nucleotide sequence