Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2567

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2565 and Pf6N2E2_2566 are separated by 33 nucleotidesPf6N2E2_2566 and Pf6N2E2_2567 are separated by 49 nucleotidesPf6N2E2_2567 and Pf6N2E2_2568 are separated by 15 nucleotidesPf6N2E2_2568 and Pf6N2E2_2569 overlap by 1 nucleotides Pf6N2E2_2565 - CheW domain protein, at 3,022,936 to 3,023,874 _2565 Pf6N2E2_2566 - Chromosome (plasmid) partitioning protein ParA, at 3,023,908 to 3,024,699 _2566 Pf6N2E2_2567 - Flagellar motor rotation protein MotB, at 3,024,749 to 3,025,633 _2567 Pf6N2E2_2568 - Flagellar motor rotation protein MotA, at 3,025,649 to 3,026,389 _2568 Pf6N2E2_2569 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 3,026,389 to 3,027,522 _2569 Position (kb) 3024 3025 3026Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3023.760 kb on - strand, within Pf6N2E2_2565at 3023.760 kb on - strand, within Pf6N2E2_2565at 3023.798 kb on - strandat 3023.811 kb on + strandat 3023.909 kb on + strandat 3024.055 kb on - strand, within Pf6N2E2_2566at 3024.056 kb on + strand, within Pf6N2E2_2566at 3024.056 kb on + strand, within Pf6N2E2_2566at 3024.056 kb on + strand, within Pf6N2E2_2566at 3024.056 kb on + strand, within Pf6N2E2_2566at 3024.056 kb on + strand, within Pf6N2E2_2566at 3024.057 kb on - strand, within Pf6N2E2_2566at 3024.057 kb on - strand, within Pf6N2E2_2566at 3024.057 kb on - strand, within Pf6N2E2_2566at 3024.057 kb on - strand, within Pf6N2E2_2566at 3024.057 kb on - strand, within Pf6N2E2_2566at 3024.080 kb on + strand, within Pf6N2E2_2566at 3024.081 kb on - strand, within Pf6N2E2_2566at 3024.081 kb on - strand, within Pf6N2E2_2566at 3024.081 kb on - strand, within Pf6N2E2_2566at 3024.198 kb on + strand, within Pf6N2E2_2566at 3024.198 kb on + strand, within Pf6N2E2_2566at 3024.198 kb on + strand, within Pf6N2E2_2566at 3024.198 kb on + strand, within Pf6N2E2_2566at 3024.199 kb on - strand, within Pf6N2E2_2566at 3024.521 kb on + strand, within Pf6N2E2_2566at 3024.560 kb on + strand, within Pf6N2E2_2566at 3024.560 kb on + strand, within Pf6N2E2_2566at 3024.741 kb on + strandat 3024.741 kb on + strandat 3024.750 kb on + strandat 3024.752 kb on + strandat 3024.753 kb on - strandat 3024.753 kb on - strandat 3025.169 kb on - strand, within Pf6N2E2_2567at 3025.169 kb on - strand, within Pf6N2E2_2567at 3025.177 kb on + strand, within Pf6N2E2_2567at 3025.177 kb on + strand, within Pf6N2E2_2567at 3025.178 kb on - strand, within Pf6N2E2_2567at 3025.178 kb on - strand, within Pf6N2E2_2567at 3025.189 kb on + strand, within Pf6N2E2_2567at 3025.189 kb on + strand, within Pf6N2E2_2567at 3025.189 kb on + strand, within Pf6N2E2_2567at 3025.500 kb on + strand, within Pf6N2E2_2567at 3025.501 kb on - strand, within Pf6N2E2_2567at 3025.501 kb on - strand, within Pf6N2E2_2567at 3025.501 kb on - strand, within Pf6N2E2_2567at 3025.531 kb on - strand, within Pf6N2E2_2567at 3025.531 kb on - strand, within Pf6N2E2_2567at 3025.531 kb on - strand, within Pf6N2E2_2567at 3025.561 kb on + strandat 3025.561 kb on + strandat 3025.575 kb on + strandat 3025.818 kb on + strand, within Pf6N2E2_2568at 3025.818 kb on + strand, within Pf6N2E2_2568at 3025.819 kb on - strand, within Pf6N2E2_2568at 3025.819 kb on - strand, within Pf6N2E2_2568at 3025.819 kb on - strand, within Pf6N2E2_2568at 3025.819 kb on - strand, within Pf6N2E2_2568at 3025.819 kb on - strand, within Pf6N2E2_2568at 3025.819 kb on - strand, within Pf6N2E2_2568at 3025.886 kb on + strand, within Pf6N2E2_2568at 3025.886 kb on + strand, within Pf6N2E2_2568at 3025.887 kb on - strand, within Pf6N2E2_2568at 3025.887 kb on - strand, within Pf6N2E2_2568at 3025.992 kb on + strand, within Pf6N2E2_2568at 3026.070 kb on + strand, within Pf6N2E2_2568at 3026.108 kb on + strand, within Pf6N2E2_2568at 3026.108 kb on + strand, within Pf6N2E2_2568at 3026.109 kb on - strand, within Pf6N2E2_2568at 3026.328 kb on + strandat 3026.329 kb on - strandat 3026.329 kb on - strandat 3026.415 kb on + strandat 3026.415 kb on + strandat 3026.484 kb on - strandat 3026.484 kb on - strandat 3026.484 kb on - strandat 3026.484 kb on - strandat 3026.607 kb on - strand, within Pf6N2E2_2569at 3026.631 kb on + strand, within Pf6N2E2_2569at 3026.631 kb on + strand, within Pf6N2E2_2569at 3026.631 kb on + strand, within Pf6N2E2_2569at 3026.631 kb on + strand, within Pf6N2E2_2569at 3026.631 kb on + strand, within Pf6N2E2_2569at 3026.632 kb on - strand, within Pf6N2E2_2569at 3026.632 kb on - strand, within Pf6N2E2_2569at 3026.632 kb on - strand, within Pf6N2E2_2569at 3026.632 kb on - strand, within Pf6N2E2_2569

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,023,760 - Pf6N2E2_2565 0.88 -2.1
3,023,760 - Pf6N2E2_2565 0.88 -1.1
3,023,798 - -0.4
3,023,811 + -2.5
3,023,909 + -3.5
3,024,055 - Pf6N2E2_2566 0.19 -0.8
3,024,056 + Pf6N2E2_2566 0.19 -2.2
3,024,056 + Pf6N2E2_2566 0.19 -3.2
3,024,056 + Pf6N2E2_2566 0.19 -2.3
3,024,056 + Pf6N2E2_2566 0.19 +0.8
3,024,056 + Pf6N2E2_2566 0.19 -3.5
3,024,057 - Pf6N2E2_2566 0.19 -1.3
3,024,057 - Pf6N2E2_2566 0.19 -0.7
3,024,057 - Pf6N2E2_2566 0.19 -0.9
3,024,057 - Pf6N2E2_2566 0.19 -1.7
3,024,057 - Pf6N2E2_2566 0.19 -1.9
3,024,080 + Pf6N2E2_2566 0.22 -3.3
3,024,081 - Pf6N2E2_2566 0.22 -0.6
3,024,081 - Pf6N2E2_2566 0.22 -0.2
3,024,081 - Pf6N2E2_2566 0.22 -2.6
3,024,198 + Pf6N2E2_2566 0.37 -2.5
3,024,198 + Pf6N2E2_2566 0.37 -1.9
3,024,198 + Pf6N2E2_2566 0.37 -2.4
3,024,198 + Pf6N2E2_2566 0.37 -2.6
3,024,199 - Pf6N2E2_2566 0.37 -1.3
3,024,521 + Pf6N2E2_2566 0.77 -3.6
3,024,560 + Pf6N2E2_2566 0.82 -1.6
3,024,560 + Pf6N2E2_2566 0.82 -1.9
3,024,741 + -2.4
3,024,741 + -1.4
3,024,750 + -1.2
3,024,752 + -4.0
3,024,753 - +2.3
3,024,753 - -1.3
3,025,169 - Pf6N2E2_2567 0.47 -3.4
3,025,169 - Pf6N2E2_2567 0.47 -0.9
3,025,177 + Pf6N2E2_2567 0.48 -2.0
3,025,177 + Pf6N2E2_2567 0.48 -3.1
3,025,178 - Pf6N2E2_2567 0.48 -1.4
3,025,178 - Pf6N2E2_2567 0.48 -1.8
3,025,189 + Pf6N2E2_2567 0.50 -1.8
3,025,189 + Pf6N2E2_2567 0.50 -3.6
3,025,189 + Pf6N2E2_2567 0.50 -2.2
3,025,500 + Pf6N2E2_2567 0.85 -3.8
3,025,501 - Pf6N2E2_2567 0.85 -2.5
3,025,501 - Pf6N2E2_2567 0.85 -0.1
3,025,501 - Pf6N2E2_2567 0.85 -2.5
3,025,531 - Pf6N2E2_2567 0.88 -0.8
3,025,531 - Pf6N2E2_2567 0.88 -2.0
3,025,531 - Pf6N2E2_2567 0.88 -2.3
3,025,561 + +0.4
3,025,561 + -0.6
3,025,575 + -1.9
3,025,818 + Pf6N2E2_2568 0.23 -1.0
3,025,818 + Pf6N2E2_2568 0.23 -1.2
3,025,819 - Pf6N2E2_2568 0.23 -2.1
3,025,819 - Pf6N2E2_2568 0.23 -1.8
3,025,819 - Pf6N2E2_2568 0.23 -3.3
3,025,819 - Pf6N2E2_2568 0.23 -2.9
3,025,819 - Pf6N2E2_2568 0.23 -2.7
3,025,819 - Pf6N2E2_2568 0.23 -1.7
3,025,886 + Pf6N2E2_2568 0.32 -3.3
3,025,886 + Pf6N2E2_2568 0.32 -3.8
3,025,887 - Pf6N2E2_2568 0.32 -3.2
3,025,887 - Pf6N2E2_2568 0.32 -1.2
3,025,992 + Pf6N2E2_2568 0.46 -3.2
3,026,070 + Pf6N2E2_2568 0.57 -0.3
3,026,108 + Pf6N2E2_2568 0.62 -1.0
3,026,108 + Pf6N2E2_2568 0.62 -1.8
3,026,109 - Pf6N2E2_2568 0.62 -2.3
3,026,328 + -2.0
3,026,329 - -4.3
3,026,329 - -2.1
3,026,415 + -3.4
3,026,415 + -1.3
3,026,484 - -4.0
3,026,484 - -2.5
3,026,484 - -2.2
3,026,484 - -2.5
3,026,607 - Pf6N2E2_2569 0.19 -3.4
3,026,631 + Pf6N2E2_2569 0.21 -2.6
3,026,631 + Pf6N2E2_2569 0.21 -2.6
3,026,631 + Pf6N2E2_2569 0.21 -2.6
3,026,631 + Pf6N2E2_2569 0.21 +0.7
3,026,631 + Pf6N2E2_2569 0.21 -3.6
3,026,632 - Pf6N2E2_2569 0.21 -3.7
3,026,632 - Pf6N2E2_2569 0.21 -2.7
3,026,632 - Pf6N2E2_2569 0.21 -1.8
3,026,632 - Pf6N2E2_2569 0.21 -2.2

Or see this region's nucleotide sequence