Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2470 and Pf6N2E2_2471 are separated by 164 nucleotides Pf6N2E2_2471 and Pf6N2E2_2472 are separated by 202 nucleotides Pf6N2E2_2472 and Pf6N2E2_2473 are separated by 319 nucleotides
Pf6N2E2_2470 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 2,918,306 to 2,919,382
_2470
Pf6N2E2_2471 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 2,919,547 to 2,919,828
_2471
Pf6N2E2_2472 - hypothetical protein, at 2,920,031 to 2,920,144
_2472
Pf6N2E2_2473 - Major outer membrane lipoprotein I, at 2,920,464 to 2,920,715
_2473
Position (kb)
2919
2920 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.571 kb on - strand, within Pf6N2E2_2470 at 2918.571 kb on - strand, within Pf6N2E2_2470 at 2918.588 kb on + strand, within Pf6N2E2_2470 at 2918.588 kb on + strand, within Pf6N2E2_2470 at 2918.588 kb on + strand, within Pf6N2E2_2470 at 2918.589 kb on - strand, within Pf6N2E2_2470 at 2918.642 kb on + strand, within Pf6N2E2_2470 at 2918.642 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.990 kb on + strand, within Pf6N2E2_2470 at 2918.991 kb on - strand, within Pf6N2E2_2470 at 2919.032 kb on + strand, within Pf6N2E2_2470 at 2919.033 kb on - strand, within Pf6N2E2_2470 at 2919.033 kb on - strand, within Pf6N2E2_2470 at 2919.272 kb on + strand, within Pf6N2E2_2470 at 2919.273 kb on - strand, within Pf6N2E2_2470 at 2919.417 kb on + strand at 2919.417 kb on + strand at 2919.417 kb on + strand at 2919.417 kb on + strand at 2919.418 kb on - strand at 2919.418 kb on - strand at 2919.451 kb on + strand at 2919.451 kb on + strand at 2919.451 kb on + strand at 2919.451 kb on + strand at 2919.452 kb on - strand at 2919.452 kb on - strand at 2919.452 kb on - strand at 2919.452 kb on - strand at 2919.478 kb on + strand at 2919.495 kb on - strand at 2919.584 kb on + strand, within Pf6N2E2_2471 at 2919.584 kb on + strand, within Pf6N2E2_2471 at 2919.584 kb on + strand, within Pf6N2E2_2471 at 2919.585 kb on - strand, within Pf6N2E2_2471 at 2919.585 kb on - strand, within Pf6N2E2_2471 at 2919.585 kb on - strand, within Pf6N2E2_2471 at 2919.585 kb on - strand, within Pf6N2E2_2471 at 2919.596 kb on + strand, within Pf6N2E2_2471 at 2919.596 kb on + strand, within Pf6N2E2_2471 at 2919.596 kb on + strand, within Pf6N2E2_2471 at 2919.596 kb on + strand, within Pf6N2E2_2471 at 2919.597 kb on - strand, within Pf6N2E2_2471 at 2919.597 kb on - strand, within Pf6N2E2_2471 at 2919.597 kb on - strand, within Pf6N2E2_2471 at 2919.639 kb on - strand, within Pf6N2E2_2471 at 2919.639 kb on - strand, within Pf6N2E2_2471 at 2919.639 kb on - strand, within Pf6N2E2_2471 at 2919.710 kb on + strand, within Pf6N2E2_2471 at 2919.711 kb on - strand, within Pf6N2E2_2471 at 2919.711 kb on - strand, within Pf6N2E2_2471 at 2919.711 kb on - strand, within Pf6N2E2_2471 at 2919.715 kb on - strand, within Pf6N2E2_2471 at 2919.743 kb on + strand, within Pf6N2E2_2471 at 2919.743 kb on + strand, within Pf6N2E2_2471 at 2919.743 kb on + strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.753 kb on - strand, within Pf6N2E2_2471 at 2919.832 kb on + strand at 2919.833 kb on - strand at 2919.861 kb on + strand at 2919.861 kb on + strand at 2919.867 kb on - strand at 2919.895 kb on + strand at 2919.895 kb on + strand at 2919.895 kb on + strand at 2919.895 kb on + strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.965 kb on + strand at 2919.965 kb on + strand at 2919.965 kb on + strand at 2919.965 kb on + strand at 2919.966 kb on - strand at 2919.966 kb on - strand at 2919.966 kb on - strand at 2919.968 kb on + strand at 2919.968 kb on + strand at 2919.968 kb on + strand at 2919.968 kb on + strand at 2919.969 kb on - strand at 2920.059 kb on + strand, within Pf6N2E2_2472 at 2920.060 kb on - strand, within Pf6N2E2_2472 at 2920.077 kb on + strand, within Pf6N2E2_2472 at 2920.078 kb on - strand, within Pf6N2E2_2472 at 2920.121 kb on - strand, within Pf6N2E2_2472 at 2920.124 kb on + strand, within Pf6N2E2_2472 at 2920.125 kb on - strand, within Pf6N2E2_2472 at 2920.139 kb on + strand at 2920.139 kb on + strand at 2920.140 kb on - strand at 2920.224 kb on + strand at 2920.224 kb on + strand at 2920.225 kb on - strand at 2920.373 kb on - strand at 2920.376 kb on + strand at 2920.376 kb on + strand at 2920.377 kb on - strand at 2920.379 kb on + strand at 2920.379 kb on + strand at 2920.379 kb on + strand at 2920.380 kb on - strand at 2920.380 kb on - strand at 2920.385 kb on + strand at 2920.385 kb on + strand at 2920.392 kb on + strand at 2920.395 kb on + strand at 2920.395 kb on + strand at 2920.395 kb on + strand at 2920.395 kb on + strand at 2920.395 kb on + strand at 2920.395 kb on + strand at 2920.395 kb on + strand at 2920.399 kb on + strand at 2920.439 kb on + strand at 2920.439 kb on + strand at 2920.439 kb on + strand at 2920.439 kb on + strand at 2920.439 kb on + strand at 2920.439 kb on + strand at 2920.440 kb on - strand at 2920.440 kb on - strand at 2920.608 kb on + strand, within Pf6N2E2_2473 at 2920.713 kb on + strand at 2920.713 kb on + strand at 2920.713 kb on + strand at 2920.713 kb on + strand at 2920.713 kb on + strand at 2920.714 kb on - strand at 2920.716 kb on + strand at 2920.717 kb on - strand at 2920.806 kb on + strand at 2920.806 kb on + strand at 2920.807 kb on - strand at 2920.807 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,918,570 + Pf6N2E2_2470 0.25 -0.1 2,918,570 + Pf6N2E2_2470 0.25 +1.1 2,918,570 + Pf6N2E2_2470 0.25 -1.4 2,918,570 + Pf6N2E2_2470 0.25 +1.8 2,918,571 - Pf6N2E2_2470 0.25 +1.7 2,918,571 - Pf6N2E2_2470 0.25 -0.1 2,918,588 + Pf6N2E2_2470 0.26 +1.0 2,918,588 + Pf6N2E2_2470 0.26 +0.2 2,918,588 + Pf6N2E2_2470 0.26 +1.7 2,918,589 - Pf6N2E2_2470 0.26 +1.7 2,918,642 + Pf6N2E2_2470 0.31 -0.5 2,918,642 + Pf6N2E2_2470 0.31 +0.9 2,918,762 + Pf6N2E2_2470 0.42 +1.3 2,918,762 + Pf6N2E2_2470 0.42 +0.4 2,918,762 + Pf6N2E2_2470 0.42 +0.2 2,918,762 + Pf6N2E2_2470 0.42 -1.7 2,918,763 - Pf6N2E2_2470 0.42 -0.6 2,918,763 - Pf6N2E2_2470 0.42 -0.8 2,918,763 - Pf6N2E2_2470 0.42 +0.3 2,918,763 - Pf6N2E2_2470 0.42 -0.9 2,918,990 + Pf6N2E2_2470 0.64 +0.3 2,918,991 - Pf6N2E2_2470 0.64 +0.9 2,919,032 + Pf6N2E2_2470 0.67 -1.9 2,919,033 - Pf6N2E2_2470 0.68 +0.7 2,919,033 - Pf6N2E2_2470 0.68 -0.6 2,919,272 + Pf6N2E2_2470 0.90 +0.2 2,919,273 - Pf6N2E2_2470 0.90 +1.0 2,919,417 + -0.4 2,919,417 + +0.2 2,919,417 + +0.6 2,919,417 + -0.2 2,919,418 - +1.5 2,919,418 - +1.7 2,919,451 + -0.4 2,919,451 + +1.9 2,919,451 + +0.9 2,919,451 + +0.7 2,919,452 - +0.1 2,919,452 - +1.9 2,919,452 - +0.8 2,919,452 - +2.2 2,919,478 + -1.2 2,919,495 - +1.7 2,919,584 + Pf6N2E2_2471 0.13 +0.8 2,919,584 + Pf6N2E2_2471 0.13 +2.4 2,919,584 + Pf6N2E2_2471 0.13 -1.7 2,919,585 - Pf6N2E2_2471 0.13 +2.5 2,919,585 - Pf6N2E2_2471 0.13 +0.2 2,919,585 - Pf6N2E2_2471 0.13 +2.2 2,919,585 - Pf6N2E2_2471 0.13 +1.8 2,919,596 + Pf6N2E2_2471 0.17 -0.9 2,919,596 + Pf6N2E2_2471 0.17 -0.3 2,919,596 + Pf6N2E2_2471 0.17 -2.3 2,919,596 + Pf6N2E2_2471 0.17 +1.7 2,919,597 - Pf6N2E2_2471 0.18 +2.1 2,919,597 - Pf6N2E2_2471 0.18 +1.1 2,919,597 - Pf6N2E2_2471 0.18 +3.1 2,919,639 - Pf6N2E2_2471 0.33 +1.0 2,919,639 - Pf6N2E2_2471 0.33 +2.0 2,919,639 - Pf6N2E2_2471 0.33 +1.5 2,919,710 + Pf6N2E2_2471 0.58 +1.9 2,919,711 - Pf6N2E2_2471 0.58 +1.3 2,919,711 - Pf6N2E2_2471 0.58 +1.4 2,919,711 - Pf6N2E2_2471 0.58 +2.7 2,919,715 - Pf6N2E2_2471 0.60 +0.5 2,919,743 + Pf6N2E2_2471 0.70 +0.4 2,919,743 + Pf6N2E2_2471 0.70 +1.6 2,919,743 + Pf6N2E2_2471 0.70 +2.9 2,919,744 - Pf6N2E2_2471 0.70 +1.0 2,919,744 - Pf6N2E2_2471 0.70 +0.8 2,919,744 - Pf6N2E2_2471 0.70 +2.7 2,919,744 - Pf6N2E2_2471 0.70 +1.6 2,919,744 - Pf6N2E2_2471 0.70 +1.9 2,919,744 - Pf6N2E2_2471 0.70 +0.6 2,919,744 - Pf6N2E2_2471 0.70 +1.4 2,919,744 - Pf6N2E2_2471 0.70 +1.9 2,919,744 - Pf6N2E2_2471 0.70 +0.8 2,919,744 - Pf6N2E2_2471 0.70 +1.1 2,919,753 - Pf6N2E2_2471 0.73 +1.9 2,919,832 + +0.8 2,919,833 - +1.8 2,919,861 + +0.8 2,919,861 + -0.1 2,919,867 - +1.4 2,919,895 + +0.2 2,919,895 + +1.1 2,919,895 + +1.8 2,919,895 + +1.0 2,919,896 - +2.2 2,919,896 - +2.2 2,919,896 - +1.7 2,919,896 - +0.7 2,919,896 - +2.4 2,919,896 - +2.3 2,919,896 - +1.2 2,919,896 - +1.8 2,919,896 - +1.2 2,919,965 + -1.4 2,919,965 + +1.2 2,919,965 + -0.1 2,919,965 + +0.1 2,919,966 - +1.4 2,919,966 - +1.7 2,919,966 - +2.0 2,919,968 + +0.9 2,919,968 + -1.0 2,919,968 + +0.6 2,919,968 + -2.2 2,919,969 - +1.4 2,920,059 + Pf6N2E2_2472 0.25 +0.9 2,920,060 - Pf6N2E2_2472 0.25 +0.5 2,920,077 + Pf6N2E2_2472 0.40 +1.5 2,920,078 - Pf6N2E2_2472 0.41 +2.0 2,920,121 - Pf6N2E2_2472 0.79 +2.2 2,920,124 + Pf6N2E2_2472 0.82 +0.0 2,920,125 - Pf6N2E2_2472 0.82 +2.8 2,920,139 + +0.7 2,920,139 + +0.3 2,920,140 - +1.5 2,920,224 + +0.3 2,920,224 + +1.8 2,920,225 - +2.3 2,920,373 - +3.1 2,920,376 + +2.6 2,920,376 + +2.5 2,920,377 - +2.5 2,920,379 + +1.3 2,920,379 + +1.7 2,920,379 + +2.7 2,920,380 - -0.3 2,920,380 - +2.0 2,920,385 + +1.7 2,920,385 + +0.6 2,920,392 + -0.8 2,920,395 + +1.0 2,920,395 + +1.9 2,920,395 + +2.2 2,920,395 + +0.6 2,920,395 + +0.8 2,920,395 + +0.6 2,920,395 + +1.7 2,920,399 + +1.7 2,920,439 + +0.5 2,920,439 + +1.8 2,920,439 + +0.8 2,920,439 + +1.7 2,920,439 + +1.7 2,920,439 + +0.6 2,920,440 - +0.9 2,920,440 - +3.9 2,920,608 + Pf6N2E2_2473 0.57 +1.4 2,920,713 + +2.2 2,920,713 + +0.9 2,920,713 + +1.3 2,920,713 + -0.7 2,920,713 + +1.7 2,920,714 - +2.0 2,920,716 + +2.5 2,920,717 - +1.9 2,920,806 + +1.5 2,920,806 + +1.7 2,920,807 - +0.9 2,920,807 - +1.1
Or see this region's nucleotide sequence