Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2328 and Pf6N2E2_2329 are separated by 858 nucleotides Pf6N2E2_2329 and Pf6N2E2_2330 are separated by 326 nucleotides
Pf6N2E2_2328 - DNA polymerase III subunits gamma and tau (EC 2.7.7.7), at 2,749,575 to 2,751,668
_2328
Pf6N2E2_2329 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 2,752,527 to 2,754,287
_2329
Pf6N2E2_2330 - Transcriptional regulator, Cro/CI family, at 2,754,614 to 2,754,928
_2330
Position (kb)
2752
2753
2754
2755 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2751.695 kb on - strand at 2751.695 kb on - strand at 2751.747 kb on + strand at 2751.748 kb on - strand at 2751.748 kb on - strand at 2751.748 kb on - strand at 2751.748 kb on - strand at 2751.757 kb on - strand at 2751.757 kb on - strand at 2751.810 kb on + strand at 2751.811 kb on - strand at 2751.904 kb on - strand at 2751.904 kb on - strand at 2751.959 kb on + strand at 2751.959 kb on + strand at 2751.959 kb on + strand at 2751.959 kb on + strand at 2751.959 kb on + strand at 2751.959 kb on + strand at 2751.959 kb on + strand at 2751.960 kb on - strand at 2751.960 kb on - strand at 2751.960 kb on - strand at 2751.960 kb on - strand at 2751.960 kb on - strand at 2751.960 kb on - strand at 2751.960 kb on - strand at 2752.058 kb on + strand at 2752.058 kb on + strand at 2752.058 kb on + strand at 2752.059 kb on - strand at 2752.059 kb on - strand at 2752.066 kb on + strand at 2752.066 kb on + strand at 2752.066 kb on + strand at 2752.067 kb on - strand at 2752.067 kb on - strand at 2752.067 kb on - strand at 2752.067 kb on - strand at 2752.102 kb on + strand at 2752.102 kb on + strand at 2752.103 kb on - strand at 2752.103 kb on - strand at 2752.202 kb on + strand at 2752.203 kb on - strand at 2752.203 kb on - strand at 2752.219 kb on + strand at 2752.219 kb on + strand at 2752.219 kb on + strand at 2752.220 kb on - strand at 2752.220 kb on - strand at 2752.220 kb on - strand at 2752.221 kb on + strand at 2752.221 kb on + strand at 2752.221 kb on + strand at 2752.222 kb on - strand at 2752.222 kb on - strand at 2752.225 kb on + strand at 2752.226 kb on - strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.323 kb on + strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.324 kb on - strand at 2752.327 kb on + strand at 2752.327 kb on + strand at 2752.327 kb on + strand at 2752.327 kb on + strand at 2752.328 kb on - strand at 2752.328 kb on - strand at 2752.328 kb on - strand at 2752.328 kb on - strand at 2752.328 kb on - strand at 2752.432 kb on + strand at 2752.432 kb on + strand at 2752.432 kb on + strand at 2752.432 kb on + strand at 2752.528 kb on + strand at 2752.529 kb on - strand at 2752.529 kb on - strand at 2752.529 kb on - strand at 2752.529 kb on - strand at 2752.672 kb on + strand at 2752.673 kb on - strand at 2752.673 kb on - strand at 2753.023 kb on - strand, within Pf6N2E2_2329 at 2753.096 kb on + strand, within Pf6N2E2_2329 at 2753.097 kb on - strand, within Pf6N2E2_2329 at 2753.097 kb on - strand, within Pf6N2E2_2329 at 2753.155 kb on - strand, within Pf6N2E2_2329 at 2753.449 kb on + strand, within Pf6N2E2_2329 at 2753.449 kb on + strand, within Pf6N2E2_2329 at 2753.450 kb on - strand, within Pf6N2E2_2329 at 2753.450 kb on - strand, within Pf6N2E2_2329 at 2753.450 kb on - strand, within Pf6N2E2_2329 at 2753.691 kb on - strand, within Pf6N2E2_2329 at 2753.854 kb on + strand, within Pf6N2E2_2329 at 2753.854 kb on + strand, within Pf6N2E2_2329 at 2753.854 kb on + strand, within Pf6N2E2_2329 at 2753.854 kb on + strand, within Pf6N2E2_2329 at 2753.854 kb on + strand, within Pf6N2E2_2329 at 2753.855 kb on - strand, within Pf6N2E2_2329 at 2753.855 kb on - strand, within Pf6N2E2_2329 at 2753.855 kb on - strand, within Pf6N2E2_2329 at 2753.855 kb on - strand, within Pf6N2E2_2329 at 2753.855 kb on - strand, within Pf6N2E2_2329 at 2753.975 kb on + strand, within Pf6N2E2_2329 at 2753.975 kb on + strand, within Pf6N2E2_2329 at 2754.016 kb on + strand, within Pf6N2E2_2329 at 2754.017 kb on - strand, within Pf6N2E2_2329 at 2754.017 kb on - strand, within Pf6N2E2_2329 at 2754.017 kb on - strand, within Pf6N2E2_2329 at 2754.023 kb on + strand, within Pf6N2E2_2329 at 2754.199 kb on + strand at 2754.199 kb on + strand at 2754.199 kb on + strand at 2754.199 kb on + strand at 2754.200 kb on - strand at 2754.200 kb on - strand at 2754.200 kb on - strand at 2754.262 kb on + strand at 2754.350 kb on + strand at 2754.350 kb on + strand at 2754.350 kb on + strand at 2754.398 kb on + strand at 2754.398 kb on + strand at 2754.399 kb on - strand at 2754.491 kb on + strand at 2754.492 kb on - strand at 2754.492 kb on - strand at 2754.539 kb on + strand at 2754.539 kb on + strand at 2754.540 kb on - strand at 2754.540 kb on - strand at 2754.670 kb on + strand, within Pf6N2E2_2330 at 2754.741 kb on - strand, within Pf6N2E2_2330 at 2754.741 kb on - strand, within Pf6N2E2_2330 at 2754.741 kb on - strand, within Pf6N2E2_2330 at 2754.741 kb on - strand, within Pf6N2E2_2330 at 2754.789 kb on + strand, within Pf6N2E2_2330 at 2754.790 kb on - strand, within Pf6N2E2_2330 at 2754.790 kb on - strand, within Pf6N2E2_2330 at 2754.790 kb on - strand, within Pf6N2E2_2330 at 2754.842 kb on + strand, within Pf6N2E2_2330 at 2754.843 kb on - strand, within Pf6N2E2_2330 at 2754.843 kb on - strand, within Pf6N2E2_2330 at 2754.858 kb on + strand, within Pf6N2E2_2330 at 2754.858 kb on + strand, within Pf6N2E2_2330 at 2754.859 kb on - strand, within Pf6N2E2_2330 at 2754.859 kb on - strand, within Pf6N2E2_2330 at 2754.943 kb on - strand at 2755.071 kb on - strand at 2755.095 kb on + strand at 2755.095 kb on + strand at 2755.096 kb on - strand at 2755.105 kb on - strand at 2755.105 kb on - strand at 2755.105 kb on - strand at 2755.122 kb on + strand at 2755.197 kb on + strand at 2755.198 kb on - strand at 2755.198 kb on - strand at 2755.198 kb on - strand at 2755.213 kb on + strand at 2755.213 kb on + strand at 2755.214 kb on - strand at 2755.239 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,751,695 - +0.3 2,751,695 - +0.9 2,751,747 + +3.0 2,751,748 - +0.1 2,751,748 - +0.6 2,751,748 - +0.6 2,751,748 - -1.1 2,751,757 - +0.8 2,751,757 - -0.1 2,751,810 + +2.2 2,751,811 - +1.3 2,751,904 - +0.9 2,751,904 - +0.0 2,751,959 + +0.4 2,751,959 + +0.8 2,751,959 + +1.1 2,751,959 + +0.2 2,751,959 + +0.5 2,751,959 + +1.7 2,751,959 + +0.8 2,751,960 - +0.7 2,751,960 - -1.2 2,751,960 - +2.1 2,751,960 - +0.3 2,751,960 - -0.1 2,751,960 - +1.1 2,751,960 - -0.1 2,752,058 + +1.3 2,752,058 + +1.8 2,752,058 + +1.3 2,752,059 - +0.1 2,752,059 - -0.2 2,752,066 + +0.7 2,752,066 + +0.1 2,752,066 + +0.5 2,752,067 - +1.7 2,752,067 - +1.5 2,752,067 - +0.1 2,752,067 - +0.2 2,752,102 + +1.1 2,752,102 + +1.9 2,752,103 - +1.2 2,752,103 - +0.9 2,752,202 + +1.4 2,752,203 - -0.4 2,752,203 - -0.9 2,752,219 + +0.2 2,752,219 + +2.0 2,752,219 + +2.1 2,752,220 - +0.6 2,752,220 - -0.8 2,752,220 - -0.8 2,752,221 + +1.0 2,752,221 + +1.3 2,752,221 + +2.1 2,752,222 - +0.2 2,752,222 - -1.0 2,752,225 + -1.3 2,752,226 - -0.8 2,752,323 + +1.5 2,752,323 + +2.1 2,752,323 + +1.4 2,752,323 + +2.2 2,752,323 + +2.6 2,752,323 + +1.1 2,752,323 + +0.5 2,752,323 + +2.9 2,752,323 + +1.5 2,752,323 + +1.4 2,752,323 + +0.9 2,752,323 + +1.1 2,752,324 - -0.3 2,752,324 - -0.6 2,752,324 - -0.7 2,752,324 - +0.4 2,752,324 - +0.3 2,752,324 - -0.2 2,752,324 - -0.8 2,752,324 - -1.6 2,752,324 - +0.8 2,752,324 - +0.9 2,752,324 - -0.1 2,752,324 - +1.2 2,752,324 - +0.6 2,752,324 - +1.9 2,752,324 - +2.4 2,752,324 - +0.2 2,752,327 + -1.7 2,752,327 + +1.1 2,752,327 + +1.1 2,752,327 + +3.0 2,752,328 - -0.2 2,752,328 - +1.7 2,752,328 - -0.3 2,752,328 - +1.1 2,752,328 - -0.4 2,752,432 + +1.5 2,752,432 + +1.6 2,752,432 + +1.4 2,752,432 + +0.6 2,752,528 + +3.3 2,752,529 - -1.3 2,752,529 - -1.3 2,752,529 - -0.8 2,752,529 - +0.4 2,752,672 + +0.5 2,752,673 - +2.7 2,752,673 - +2.6 2,753,023 - Pf6N2E2_2329 0.28 +2.1 2,753,096 + Pf6N2E2_2329 0.32 +3.5 2,753,097 - Pf6N2E2_2329 0.32 +2.1 2,753,097 - Pf6N2E2_2329 0.32 +2.4 2,753,155 - Pf6N2E2_2329 0.36 +1.7 2,753,449 + Pf6N2E2_2329 0.52 +1.3 2,753,449 + Pf6N2E2_2329 0.52 +1.9 2,753,450 - Pf6N2E2_2329 0.52 +2.6 2,753,450 - Pf6N2E2_2329 0.52 +2.9 2,753,450 - Pf6N2E2_2329 0.52 +2.3 2,753,691 - Pf6N2E2_2329 0.66 +3.0 2,753,854 + Pf6N2E2_2329 0.75 -1.8 2,753,854 + Pf6N2E2_2329 0.75 +2.9 2,753,854 + Pf6N2E2_2329 0.75 +1.3 2,753,854 + Pf6N2E2_2329 0.75 +2.0 2,753,854 + Pf6N2E2_2329 0.75 -0.9 2,753,855 - Pf6N2E2_2329 0.75 +2.9 2,753,855 - Pf6N2E2_2329 0.75 +2.6 2,753,855 - Pf6N2E2_2329 0.75 +1.7 2,753,855 - Pf6N2E2_2329 0.75 +1.9 2,753,855 - Pf6N2E2_2329 0.75 +2.2 2,753,975 + Pf6N2E2_2329 0.82 +2.7 2,753,975 + Pf6N2E2_2329 0.82 +2.7 2,754,016 + Pf6N2E2_2329 0.85 +1.8 2,754,017 - Pf6N2E2_2329 0.85 +2.4 2,754,017 - Pf6N2E2_2329 0.85 +1.5 2,754,017 - Pf6N2E2_2329 0.85 +0.0 2,754,023 + Pf6N2E2_2329 0.85 +3.0 2,754,199 + +2.3 2,754,199 + +3.0 2,754,199 + +2.2 2,754,199 + +1.1 2,754,200 - +1.1 2,754,200 - +2.6 2,754,200 - +2.2 2,754,262 + +2.0 2,754,350 + +2.9 2,754,350 + +1.1 2,754,350 + +2.1 2,754,398 + +2.8 2,754,398 + +2.3 2,754,399 - -0.7 2,754,491 + +2.1 2,754,492 - -1.2 2,754,492 - -2.9 2,754,539 + +2.2 2,754,539 + +1.9 2,754,540 - -1.1 2,754,540 - +0.3 2,754,670 + Pf6N2E2_2330 0.18 +1.8 2,754,741 - Pf6N2E2_2330 0.40 -2.5 2,754,741 - Pf6N2E2_2330 0.40 -0.4 2,754,741 - Pf6N2E2_2330 0.40 -1.7 2,754,741 - Pf6N2E2_2330 0.40 -1.5 2,754,789 + Pf6N2E2_2330 0.56 -0.2 2,754,790 - Pf6N2E2_2330 0.56 -0.8 2,754,790 - Pf6N2E2_2330 0.56 -0.8 2,754,790 - Pf6N2E2_2330 0.56 -1.9 2,754,842 + Pf6N2E2_2330 0.72 -0.4 2,754,843 - Pf6N2E2_2330 0.73 -1.5 2,754,843 - Pf6N2E2_2330 0.73 -3.0 2,754,858 + Pf6N2E2_2330 0.77 -1.0 2,754,858 + Pf6N2E2_2330 0.77 +1.3 2,754,859 - Pf6N2E2_2330 0.78 -0.2 2,754,859 - Pf6N2E2_2330 0.78 -3.3 2,754,943 - -1.1 2,755,071 - -1.4 2,755,095 + +0.5 2,755,095 + +2.0 2,755,096 - -0.3 2,755,105 - -0.0 2,755,105 - +0.2 2,755,105 - +2.5 2,755,122 + +0.1 2,755,197 + +2.4 2,755,198 - +0.0 2,755,198 - +2.1 2,755,198 - +1.1 2,755,213 + -1.5 2,755,213 + +0.7 2,755,214 - -0.7 2,755,239 - +0.7
Or see this region's nucleotide sequence