Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_167 and Pf6N2E2_168 are separated by 48 nucleotides Pf6N2E2_168 and Pf6N2E2_169 are separated by 128 nucleotides Pf6N2E2_169 and Pf6N2E2_170 are separated by 162 nucleotides Pf6N2E2_170 and Pf6N2E2_171 are separated by 68 nucleotides Pf6N2E2_171 and Pf6N2E2_172 are separated by 289 nucleotides
Pf6N2E2_167 - Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1), at 220,216 to 221,244
_167
Pf6N2E2_168 - hypothetical protein, at 221,293 to 221,490
_168
Pf6N2E2_169 - FIG00955358: hypothetical protein, at 221,619 to 221,924
_169
Pf6N2E2_170 - lipoprotein, putative, at 222,087 to 222,401
_170
Pf6N2E2_171 - Cold shock protein CspG, at 222,470 to 222,682
_171
Pf6N2E2_172 - FIG00953292: hypothetical protein, at 222,972 to 223,274
_172
Position (kb) 222
223 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 221.144 kb on - strand at 221.144 kb on - strand at 221.255 kb on + strand at 221.255 kb on + strand at 221.256 kb on - strand at 221.256 kb on - strand at 221.257 kb on + strand at 221.258 kb on - strand at 221.258 kb on - strand at 221.389 kb on + strand, within Pf6N2E2_168 at 221.389 kb on + strand, within Pf6N2E2_168 at 221.429 kb on + strand, within Pf6N2E2_168 at 221.429 kb on + strand, within Pf6N2E2_168 at 221.429 kb on + strand, within Pf6N2E2_168 at 221.430 kb on - strand, within Pf6N2E2_168 at 221.430 kb on - strand, within Pf6N2E2_168 at 221.430 kb on - strand, within Pf6N2E2_168 at 221.436 kb on + strand, within Pf6N2E2_168 at 221.436 kb on + strand, within Pf6N2E2_168 at 221.437 kb on - strand, within Pf6N2E2_168 at 221.493 kb on + strand at 221.493 kb on + strand at 221.494 kb on - strand at 221.517 kb on + strand at 221.517 kb on + strand at 221.517 kb on + strand at 221.518 kb on - strand at 221.518 kb on - strand at 221.518 kb on - strand at 221.518 kb on - strand at 221.527 kb on + strand at 221.528 kb on - strand at 221.561 kb on + strand at 221.562 kb on - strand at 221.562 kb on - strand at 221.702 kb on + strand, within Pf6N2E2_169 at 221.703 kb on - strand, within Pf6N2E2_169 at 221.736 kb on + strand, within Pf6N2E2_169 at 221.737 kb on - strand, within Pf6N2E2_169 at 221.737 kb on - strand, within Pf6N2E2_169 at 221.737 kb on - strand, within Pf6N2E2_169 at 221.737 kb on - strand, within Pf6N2E2_169 at 221.817 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.882 kb on + strand, within Pf6N2E2_169 at 221.882 kb on + strand, within Pf6N2E2_169 at 221.882 kb on + strand, within Pf6N2E2_169 at 221.883 kb on - strand, within Pf6N2E2_169 at 221.883 kb on - strand, within Pf6N2E2_169 at 221.907 kb on + strand at 221.907 kb on + strand at 221.920 kb on - strand at 222.063 kb on + strand at 222.063 kb on + strand at 222.063 kb on + strand at 222.064 kb on - strand at 222.113 kb on + strand at 222.113 kb on + strand at 222.113 kb on + strand at 222.114 kb on - strand at 222.114 kb on - strand at 222.359 kb on + strand, within Pf6N2E2_170 at 222.360 kb on - strand, within Pf6N2E2_170 at 222.455 kb on + strand at 222.472 kb on - strand at 222.588 kb on - strand, within Pf6N2E2_171 at 222.687 kb on + strand at 222.703 kb on - strand at 222.703 kb on - strand at 222.704 kb on + strand at 222.704 kb on + strand at 222.705 kb on - strand at 222.715 kb on + strand at 222.715 kb on + strand at 222.716 kb on - strand at 222.716 kb on - strand at 222.755 kb on + strand at 222.903 kb on - strand at 222.903 kb on - strand at 222.903 kb on - strand at 223.084 kb on + strand, within Pf6N2E2_172 at 223.085 kb on - strand, within Pf6N2E2_172 at 223.085 kb on - strand, within Pf6N2E2_172 at 223.177 kb on + strand, within Pf6N2E2_172 at 223.178 kb on - strand, within Pf6N2E2_172 at 223.178 kb on - strand, within Pf6N2E2_172 at 223.178 kb on - strand, within Pf6N2E2_172 at 223.178 kb on - strand, within Pf6N2E2_172 at 223.249 kb on - strand at 223.262 kb on + strand at 223.262 kb on + strand at 223.262 kb on + strand at 223.262 kb on + strand at 223.263 kb on - strand at 223.263 kb on - strand at 223.263 kb on - strand at 223.263 kb on - strand at 223.263 kb on - strand at 223.349 kb on - strand at 223.349 kb on - strand at 223.363 kb on + strand at 223.363 kb on + strand at 223.363 kb on + strand at 223.363 kb on + strand at 223.364 kb on - strand at 223.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 221,144 - +1.5 221,144 - +0.3 221,255 + +0.9 221,255 + -1.0 221,256 - +0.7 221,256 - -1.4 221,257 + +0.0 221,258 - -0.5 221,258 - -1.4 221,389 + Pf6N2E2_168 0.48 +0.9 221,389 + Pf6N2E2_168 0.48 -1.0 221,429 + Pf6N2E2_168 0.69 +1.1 221,429 + Pf6N2E2_168 0.69 +0.2 221,429 + Pf6N2E2_168 0.69 +1.2 221,430 - Pf6N2E2_168 0.69 -0.3 221,430 - Pf6N2E2_168 0.69 +0.7 221,430 - Pf6N2E2_168 0.69 -0.7 221,436 + Pf6N2E2_168 0.72 -2.2 221,436 + Pf6N2E2_168 0.72 +1.1 221,437 - Pf6N2E2_168 0.73 -1.9 221,493 + +1.5 221,493 + +1.5 221,494 - +0.5 221,517 + +1.0 221,517 + +2.7 221,517 + +0.9 221,518 - +0.1 221,518 - -0.4 221,518 - -0.4 221,518 - -1.2 221,527 + -1.2 221,528 - -0.2 221,561 + +0.6 221,562 - +0.9 221,562 - +0.0 221,702 + Pf6N2E2_169 0.27 +1.0 221,703 - Pf6N2E2_169 0.27 -0.2 221,736 + Pf6N2E2_169 0.38 +0.3 221,737 - Pf6N2E2_169 0.39 +0.2 221,737 - Pf6N2E2_169 0.39 +0.1 221,737 - Pf6N2E2_169 0.39 -1.5 221,737 - Pf6N2E2_169 0.39 +0.5 221,817 + Pf6N2E2_169 0.65 +3.0 221,865 + Pf6N2E2_169 0.80 +0.6 221,865 + Pf6N2E2_169 0.80 -0.4 221,865 + Pf6N2E2_169 0.80 +0.7 221,865 + Pf6N2E2_169 0.80 -0.4 221,865 + Pf6N2E2_169 0.80 -1.0 221,866 - Pf6N2E2_169 0.81 -0.4 221,866 - Pf6N2E2_169 0.81 -0.5 221,866 - Pf6N2E2_169 0.81 -2.0 221,866 - Pf6N2E2_169 0.81 -0.1 221,866 - Pf6N2E2_169 0.81 -0.4 221,866 - Pf6N2E2_169 0.81 +0.6 221,866 - Pf6N2E2_169 0.81 +0.8 221,866 - Pf6N2E2_169 0.81 -1.1 221,882 + Pf6N2E2_169 0.86 +0.9 221,882 + Pf6N2E2_169 0.86 +0.7 221,882 + Pf6N2E2_169 0.86 -0.3 221,883 - Pf6N2E2_169 0.86 +0.8 221,883 - Pf6N2E2_169 0.86 +1.1 221,907 + +0.7 221,907 + +2.1 221,920 - +1.6 222,063 + +1.0 222,063 + +1.4 222,063 + +0.3 222,064 - +0.6 222,113 + -1.2 222,113 + -1.2 222,113 + +0.8 222,114 - +1.8 222,114 - -1.2 222,359 + Pf6N2E2_170 0.86 -2.0 222,360 - Pf6N2E2_170 0.87 -0.7 222,455 + -0.2 222,472 - +0.8 222,588 - Pf6N2E2_171 0.55 +1.4 222,687 + -2.9 222,703 - +0.2 222,703 - +0.2 222,704 + +0.2 222,704 + +0.7 222,705 - +0.1 222,715 + -0.1 222,715 + +1.8 222,716 - +0.4 222,716 - +1.2 222,755 + -0.1 222,903 - -0.4 222,903 - +0.5 222,903 - +1.1 223,084 + Pf6N2E2_172 0.37 +0.5 223,085 - Pf6N2E2_172 0.37 -0.6 223,085 - Pf6N2E2_172 0.37 -1.7 223,177 + Pf6N2E2_172 0.68 +0.8 223,178 - Pf6N2E2_172 0.68 +0.3 223,178 - Pf6N2E2_172 0.68 +0.5 223,178 - Pf6N2E2_172 0.68 -0.5 223,178 - Pf6N2E2_172 0.68 +0.3 223,249 - +1.0 223,262 + +1.3 223,262 + +1.1 223,262 + +0.7 223,262 + -1.1 223,263 - +1.2 223,263 - +0.4 223,263 - -0.1 223,263 - +0.3 223,263 - +0.5 223,349 - -0.8 223,349 - -0.3 223,363 + +0.3 223,363 + -0.5 223,363 + +0.4 223,363 + +0.8 223,364 - -1.0 223,364 - -2.5
Or see this region's nucleotide sequence