Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1438 and Pf6N2E2_1439 are separated by 237 nucleotides Pf6N2E2_1439 and Pf6N2E2_1440 are separated by 469 nucleotides
Pf6N2E2_1438 - Methyltransferase (EC 2.1.1.-), at 1,688,548 to 1,689,132
_1438
Pf6N2E2_1439 - hypothetical protein, at 1,689,370 to 1,689,948
_1439
Pf6N2E2_1440 - Putative tRNA-m1A22 methylase, at 1,690,418 to 1,691,113
_1440
Position (kb)
1689
1690 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1688.391 kb on - strand at 1688.391 kb on - strand at 1688.391 kb on - strand at 1688.396 kb on - strand at 1688.416 kb on + strand at 1688.416 kb on + strand at 1688.417 kb on - strand at 1688.417 kb on - strand at 1688.417 kb on - strand at 1688.450 kb on + strand at 1688.507 kb on + strand at 1688.508 kb on - strand at 1688.744 kb on + strand, within Pf6N2E2_1438 at 1688.745 kb on - strand, within Pf6N2E2_1438 at 1688.821 kb on - strand, within Pf6N2E2_1438 at 1688.821 kb on - strand, within Pf6N2E2_1438 at 1688.930 kb on - strand, within Pf6N2E2_1438 at 1688.988 kb on + strand, within Pf6N2E2_1438 at 1688.989 kb on - strand, within Pf6N2E2_1438 at 1689.095 kb on - strand at 1689.109 kb on + strand at 1689.110 kb on - strand at 1689.234 kb on - strand at 1689.234 kb on - strand at 1689.324 kb on - strand at 1689.379 kb on + strand at 1689.380 kb on - strand at 1689.445 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.482 kb on - strand, within Pf6N2E2_1439 at 1689.482 kb on - strand, within Pf6N2E2_1439 at 1689.482 kb on - strand, within Pf6N2E2_1439 at 1689.672 kb on - strand, within Pf6N2E2_1439 at 1689.672 kb on - strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.706 kb on + strand, within Pf6N2E2_1439 at 1689.707 kb on - strand, within Pf6N2E2_1439 at 1689.781 kb on + strand, within Pf6N2E2_1439 at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.098 kb on - strand at 1690.098 kb on - strand at 1690.098 kb on - strand at 1690.227 kb on + strand at 1690.228 kb on - strand at 1690.277 kb on - strand at 1690.277 kb on - strand at 1690.330 kb on - strand at 1690.330 kb on - strand at 1690.340 kb on + strand at 1690.351 kb on - strand at 1690.596 kb on + strand, within Pf6N2E2_1440 at 1690.596 kb on + strand, within Pf6N2E2_1440 at 1690.614 kb on + strand, within Pf6N2E2_1440 at 1690.615 kb on - strand, within Pf6N2E2_1440 at 1690.751 kb on + strand, within Pf6N2E2_1440 at 1690.751 kb on + strand, within Pf6N2E2_1440 at 1690.752 kb on - strand, within Pf6N2E2_1440 at 1690.752 kb on - strand, within Pf6N2E2_1440
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,688,391 - -0.1 1,688,391 - +1.4 1,688,391 - -0.8 1,688,396 - -1.9 1,688,416 + -2.2 1,688,416 + -2.2 1,688,417 - -1.0 1,688,417 - -0.6 1,688,417 - +1.4 1,688,450 + -0.3 1,688,507 + -1.3 1,688,508 - -1.5 1,688,744 + Pf6N2E2_1438 0.34 +2.0 1,688,745 - Pf6N2E2_1438 0.34 +0.3 1,688,821 - Pf6N2E2_1438 0.47 -0.5 1,688,821 - Pf6N2E2_1438 0.47 +0.3 1,688,930 - Pf6N2E2_1438 0.65 +0.4 1,688,988 + Pf6N2E2_1438 0.75 -0.7 1,688,989 - Pf6N2E2_1438 0.75 +0.3 1,689,095 - -0.0 1,689,109 + -1.2 1,689,110 - +1.1 1,689,234 - -0.0 1,689,234 - +0.7 1,689,324 - +0.5 1,689,379 + -0.1 1,689,380 - -2.2 1,689,445 + Pf6N2E2_1439 0.13 -0.2 1,689,481 + Pf6N2E2_1439 0.19 -0.9 1,689,481 + Pf6N2E2_1439 0.19 -0.8 1,689,481 + Pf6N2E2_1439 0.19 +1.7 1,689,481 + Pf6N2E2_1439 0.19 -1.0 1,689,482 - Pf6N2E2_1439 0.19 -1.3 1,689,482 - Pf6N2E2_1439 0.19 -0.2 1,689,482 - Pf6N2E2_1439 0.19 -0.7 1,689,672 - Pf6N2E2_1439 0.52 +0.5 1,689,672 - Pf6N2E2_1439 0.52 -0.7 1,689,685 + Pf6N2E2_1439 0.54 +1.4 1,689,685 + Pf6N2E2_1439 0.54 -1.4 1,689,685 + Pf6N2E2_1439 0.54 +0.5 1,689,685 + Pf6N2E2_1439 0.54 +1.8 1,689,685 + Pf6N2E2_1439 0.54 +0.2 1,689,686 - Pf6N2E2_1439 0.55 -0.2 1,689,686 - Pf6N2E2_1439 0.55 +0.3 1,689,686 - Pf6N2E2_1439 0.55 -1.7 1,689,686 - Pf6N2E2_1439 0.55 -1.5 1,689,706 + Pf6N2E2_1439 0.58 -1.9 1,689,707 - Pf6N2E2_1439 0.58 -0.0 1,689,781 + Pf6N2E2_1439 0.71 -1.2 1,690,094 - +0.7 1,690,094 - -1.3 1,690,094 - -0.5 1,690,094 - +1.6 1,690,094 - +0.1 1,690,094 - -0.7 1,690,098 - +0.3 1,690,098 - +0.9 1,690,098 - +1.1 1,690,227 + -1.0 1,690,228 - -2.6 1,690,277 - -0.6 1,690,277 - +0.6 1,690,330 - +0.8 1,690,330 - +1.0 1,690,340 + +1.3 1,690,351 - +0.1 1,690,596 + Pf6N2E2_1440 0.26 -0.7 1,690,596 + Pf6N2E2_1440 0.26 -0.2 1,690,614 + Pf6N2E2_1440 0.28 +0.6 1,690,615 - Pf6N2E2_1440 0.28 -1.2 1,690,751 + Pf6N2E2_1440 0.48 +1.5 1,690,751 + Pf6N2E2_1440 0.48 +0.3 1,690,752 - Pf6N2E2_1440 0.48 +0.1 1,690,752 - Pf6N2E2_1440 0.48 +2.0
Or see this region's nucleotide sequence