Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2081 and Ac3H11_2082 are separated by 99 nucleotides Ac3H11_2082 and Ac3H11_2083 are separated by 38 nucleotides Ac3H11_2083 and Ac3H11_2084 are separated by 234 nucleotides
Ac3H11_2081 - Gluconolactonase (EC 3.1.1.17), at 306,473 to 307,423
_2081
Ac3H11_2082 - Phosphogluconate dehydratase (EC 4.2.1.12), at 307,523 to 309,292
_2082
Ac3H11_2083 - 4-Hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14), at 309,331 to 309,960
_2083
Ac3H11_2084 - protein of unknown function DUF805, at 310,195 to 310,497
_2084
Position (kb)
307
308
309
310 Strain fitness (log2 ratio)
-2
-1
0
1 at 307.469 kb on + strand at 309.290 kb on + strand at 309.290 kb on + strand at 309.290 kb on + strand at 309.291 kb on - strand at 309.291 kb on - strand at 309.291 kb on - strand at 309.291 kb on - strand at 309.291 kb on - strand at 309.303 kb on - strand at 309.314 kb on + strand at 309.314 kb on + strand at 309.314 kb on + strand at 309.314 kb on + strand at 309.314 kb on + strand at 309.314 kb on + strand at 309.314 kb on + strand at 309.314 kb on + strand at 309.314 kb on + strand at 309.315 kb on - strand at 309.315 kb on - strand at 309.315 kb on - strand at 309.315 kb on - strand at 309.315 kb on - strand at 309.315 kb on - strand at 309.315 kb on - strand at 309.315 kb on - strand at 309.318 kb on + strand at 309.318 kb on + strand at 309.318 kb on + strand at 309.318 kb on + strand at 309.319 kb on - strand at 309.393 kb on - strand at 309.808 kb on - strand at 309.829 kb on + strand, within Ac3H11_2083 at 309.829 kb on + strand, within Ac3H11_2083 at 309.981 kb on + strand at 310.016 kb on - strand at 310.028 kb on - strand at 310.127 kb on - strand at 310.195 kb on - strand at 310.234 kb on + strand, within Ac3H11_2084 at 310.234 kb on + strand, within Ac3H11_2084
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 307,469 + +1.4 309,290 + +0.0 309,290 + -0.7 309,290 + -0.3 309,291 - +0.0 309,291 - -0.3 309,291 - +0.2 309,291 - +0.1 309,291 - +0.0 309,303 - +0.3 309,314 + +0.7 309,314 + +0.9 309,314 + -0.4 309,314 + +0.4 309,314 + -0.4 309,314 + +0.7 309,314 + +0.5 309,314 + +0.4 309,314 + +0.1 309,315 - +0.7 309,315 - -0.3 309,315 - -0.1 309,315 - -0.3 309,315 - +0.2 309,315 - -2.0 309,315 - -2.4 309,315 - -0.3 309,318 + -0.0 309,318 + +1.2 309,318 + +0.2 309,318 + +0.4 309,319 - -0.9 309,393 - +0.9 309,808 - -0.1 309,829 + Ac3H11_2083 0.79 +1.3 309,829 + Ac3H11_2083 0.79 -0.4 309,981 + -0.0 310,016 - +0.7 310,028 - +0.3 310,127 - +0.8 310,195 - +0.2 310,234 + Ac3H11_2084 0.13 +0.0 310,234 + Ac3H11_2084 0.13 +0.0
Or see this region's nucleotide sequence