Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS18010 and ABZR86_RS18015 are separated by 129 nucleotides ABZR86_RS18015 and ABZR86_RS18020 are separated by 104 nucleotides ABZR86_RS18020 and ABZR86_RS18025 are separated by 3 nucleotides ABZR86_RS18025 and ABZR86_RS18030 overlap by 4 nucleotides
ABZR86_RS18010: ABZR86_RS18010 - aldo/keto reductase, at 16,447 to 17,259
_RS18010
ABZR86_RS18015: ABZR86_RS18015 - TetR/AcrR family transcriptional regulator, at 17,389 to 18,003
_RS18015
ABZR86_RS18020: ABZR86_RS18020 - MBL fold metallo-hydrolase, at 18,108 to 19,235
_RS18020
ABZR86_RS18025: ABZR86_RS18025 - poly-beta-1,6-N-acetyl-D-glucosamine biosynthesis protein PgaD, at 19,239 to 19,709
_RS18025
ABZR86_RS18030: ABZR86_RS18030 - poly-beta-1,6-N-acetyl-D-glucosamine synthase, at 19,706 to 20,953
_RS18030
Position (kb)
18
19
20 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 17.322 kb on - strand at 17.330 kb on + strand at 17.330 kb on + strand at 17.330 kb on + strand at 17.330 kb on + strand at 17.330 kb on + strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.336 kb on + strand at 17.336 kb on + strand at 17.337 kb on - strand at 17.337 kb on - strand at 17.337 kb on - strand at 17.337 kb on - strand at 17.396 kb on + strand at 17.413 kb on - strand at 17.765 kb on + strand, within ABZR86_RS18015 at 17.766 kb on - strand, within ABZR86_RS18015 at 17.852 kb on + strand, within ABZR86_RS18015 at 17.853 kb on - strand, within ABZR86_RS18015 at 18.107 kb on + strand at 18.568 kb on - strand, within ABZR86_RS18020 at 18.597 kb on + strand, within ABZR86_RS18020 at 18.598 kb on - strand, within ABZR86_RS18020 at 18.924 kb on + strand, within ABZR86_RS18020 at 18.924 kb on + strand, within ABZR86_RS18020 at 18.942 kb on + strand, within ABZR86_RS18020 at 19.445 kb on - strand, within ABZR86_RS18025 at 19.445 kb on - strand, within ABZR86_RS18025 at 19.445 kb on - strand, within ABZR86_RS18025 at 19.549 kb on + strand, within ABZR86_RS18025 at 19.842 kb on + strand, within ABZR86_RS18030 at 19.842 kb on + strand, within ABZR86_RS18030 at 19.842 kb on + strand, within ABZR86_RS18030 at 19.843 kb on - strand, within ABZR86_RS18030 at 20.000 kb on + strand, within ABZR86_RS18030 at 20.050 kb on - strand, within ABZR86_RS18030 at 20.050 kb on - strand, within ABZR86_RS18030 at 20.107 kb on - strand, within ABZR86_RS18030 at 20.107 kb on - strand, within ABZR86_RS18030
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 17,322 - -3.4 17,330 + -0.7 17,330 + +1.2 17,330 + +1.0 17,330 + +1.1 17,330 + -2.3 17,331 - -0.9 17,331 - +1.3 17,331 - -2.5 17,331 - +1.1 17,331 - +0.0 17,331 - +0.4 17,331 - -0.6 17,331 - -2.7 17,331 - -0.3 17,336 + -3.9 17,336 + +0.1 17,337 - +2.4 17,337 - -0.2 17,337 - +1.0 17,337 - -2.0 17,396 + -0.1 17,413 - -0.7 17,765 + ABZR86_RS18015 0.61 +1.1 17,766 - ABZR86_RS18015 0.61 -3.1 17,852 + ABZR86_RS18015 0.75 +0.9 17,853 - ABZR86_RS18015 0.75 +1.5 18,107 + -3.2 18,568 - ABZR86_RS18020 0.41 -3.3 18,597 + ABZR86_RS18020 0.43 +0.2 18,598 - ABZR86_RS18020 0.43 -0.5 18,924 + ABZR86_RS18020 0.72 +0.6 18,924 + ABZR86_RS18020 0.72 -0.5 18,942 + ABZR86_RS18020 0.74 -2.0 19,445 - ABZR86_RS18025 0.44 +0.9 19,445 - ABZR86_RS18025 0.44 -0.3 19,445 - ABZR86_RS18025 0.44 +1.7 19,549 + ABZR86_RS18025 0.66 -0.7 19,842 + ABZR86_RS18030 0.11 -1.9 19,842 + ABZR86_RS18030 0.11 +1.0 19,842 + ABZR86_RS18030 0.11 -0.2 19,843 - ABZR86_RS18030 0.11 -0.9 20,000 + ABZR86_RS18030 0.24 -2.9 20,050 - ABZR86_RS18030 0.28 +0.5 20,050 - ABZR86_RS18030 0.28 +0.9 20,107 - ABZR86_RS18030 0.32 -2.6 20,107 - ABZR86_RS18030 0.32 +0.5
Or see this region's nucleotide sequence