Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS13220 and ABZR86_RS13225 are separated by 60 nucleotides ABZR86_RS13225 and ABZR86_RS13230 are separated by 17 nucleotides ABZR86_RS13230 and ABZR86_RS13235 are separated by 84 nucleotides ABZR86_RS13235 and ABZR86_RS13240 are separated by 70 nucleotides
ABZR86_RS13220: ABZR86_RS13220 - AraC family transcriptional regulator, at 2,969,499 to 2,970,410
_RS13220
ABZR86_RS13225: ABZR86_RS13225 - HAD family hydrolase, at 2,970,471 to 2,971,160
_RS13225
ABZR86_RS13230: ABZR86_RS13230 - DUF4440 domain-containing protein, at 2,971,178 to 2,971,564
_RS13230
ABZR86_RS13235: ABZR86_RS13235 - hypothetical protein, at 2,971,649 to 2,971,978
_RS13235
ABZR86_RS13240: ABZR86_RS13240 - class I SAM-dependent methyltransferase, at 2,972,049 to 2,972,666
_RS13240
Position (kb)
2970
2971
2972 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2969.498 kb on + strand at 2969.498 kb on + strand at 2969.498 kb on + strand at 2969.498 kb on + strand at 2969.502 kb on + strand at 2969.502 kb on + strand at 2969.502 kb on + strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.504 kb on + strand at 2969.646 kb on + strand, within ABZR86_RS13220 at 2969.646 kb on + strand, within ABZR86_RS13220 at 2969.647 kb on - strand, within ABZR86_RS13220 at 2969.823 kb on + strand, within ABZR86_RS13220 at 2969.824 kb on - strand, within ABZR86_RS13220 at 2969.824 kb on - strand, within ABZR86_RS13220 at 2970.018 kb on + strand, within ABZR86_RS13220 at 2970.213 kb on + strand, within ABZR86_RS13220 at 2970.214 kb on - strand, within ABZR86_RS13220 at 2970.222 kb on + strand, within ABZR86_RS13220 at 2970.223 kb on - strand, within ABZR86_RS13220 at 2970.321 kb on + strand at 2970.321 kb on + strand at 2970.322 kb on - strand at 2970.338 kb on + strand at 2970.339 kb on - strand at 2970.406 kb on + strand at 2970.406 kb on + strand at 2970.407 kb on - strand at 2970.644 kb on + strand, within ABZR86_RS13225 at 2970.783 kb on + strand, within ABZR86_RS13225 at 2970.783 kb on + strand, within ABZR86_RS13225 at 2971.328 kb on + strand, within ABZR86_RS13230 at 2971.340 kb on + strand, within ABZR86_RS13230 at 2971.340 kb on + strand, within ABZR86_RS13230 at 2971.340 kb on + strand, within ABZR86_RS13230 at 2971.341 kb on - strand, within ABZR86_RS13230 at 2971.341 kb on - strand, within ABZR86_RS13230 at 2971.341 kb on - strand, within ABZR86_RS13230 at 2971.341 kb on - strand, within ABZR86_RS13230 at 2971.439 kb on + strand, within ABZR86_RS13230 at 2971.439 kb on + strand, within ABZR86_RS13230 at 2971.439 kb on + strand, within ABZR86_RS13230 at 2971.439 kb on + strand, within ABZR86_RS13230 at 2971.451 kb on + strand, within ABZR86_RS13230 at 2971.452 kb on - strand, within ABZR86_RS13230 at 2971.452 kb on - strand, within ABZR86_RS13230 at 2971.452 kb on - strand, within ABZR86_RS13230 at 2971.452 kb on - strand, within ABZR86_RS13230 at 2971.452 kb on - strand, within ABZR86_RS13230 at 2971.452 kb on - strand, within ABZR86_RS13230 at 2971.633 kb on + strand at 2971.634 kb on - strand at 2971.641 kb on + strand at 2971.641 kb on + strand at 2971.641 kb on + strand at 2971.642 kb on - strand at 2971.642 kb on - strand at 2971.796 kb on + strand, within ABZR86_RS13235 at 2971.796 kb on + strand, within ABZR86_RS13235 at 2971.797 kb on - strand, within ABZR86_RS13235 at 2971.797 kb on - strand, within ABZR86_RS13235 at 2971.982 kb on - strand at 2971.982 kb on - strand at 2971.996 kb on + strand at 2971.997 kb on - strand at 2971.997 kb on - strand at 2972.141 kb on - strand, within ABZR86_RS13240
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 2,969,498 + +1.2 2,969,498 + +1.9 2,969,498 + -0.6 2,969,498 + -0.7 2,969,502 + -0.4 2,969,502 + +0.2 2,969,502 + -0.0 2,969,503 - +0.3 2,969,503 - +0.2 2,969,503 - -0.1 2,969,503 - -0.6 2,969,503 - -0.7 2,969,503 - +0.2 2,969,503 - -1.3 2,969,503 - +0.2 2,969,503 - +2.1 2,969,503 - -0.9 2,969,504 + -0.2 2,969,646 + ABZR86_RS13220 0.16 -0.0 2,969,646 + ABZR86_RS13220 0.16 -0.8 2,969,647 - ABZR86_RS13220 0.16 +1.5 2,969,823 + ABZR86_RS13220 0.36 -0.1 2,969,824 - ABZR86_RS13220 0.36 -1.5 2,969,824 - ABZR86_RS13220 0.36 +1.4 2,970,018 + ABZR86_RS13220 0.57 -1.2 2,970,213 + ABZR86_RS13220 0.78 +1.1 2,970,214 - ABZR86_RS13220 0.78 -1.2 2,970,222 + ABZR86_RS13220 0.79 +0.4 2,970,223 - ABZR86_RS13220 0.79 +1.7 2,970,321 + +0.6 2,970,321 + -1.2 2,970,322 - +0.9 2,970,338 + -0.2 2,970,339 - +1.9 2,970,406 + -2.3 2,970,406 + -0.9 2,970,407 - -0.5 2,970,644 + ABZR86_RS13225 0.25 -1.9 2,970,783 + ABZR86_RS13225 0.45 +1.1 2,970,783 + ABZR86_RS13225 0.45 -2.3 2,971,328 + ABZR86_RS13230 0.39 +0.7 2,971,340 + ABZR86_RS13230 0.42 +0.0 2,971,340 + ABZR86_RS13230 0.42 -3.8 2,971,340 + ABZR86_RS13230 0.42 +0.3 2,971,341 - ABZR86_RS13230 0.42 -0.5 2,971,341 - ABZR86_RS13230 0.42 -1.0 2,971,341 - ABZR86_RS13230 0.42 -1.2 2,971,341 - ABZR86_RS13230 0.42 -0.1 2,971,439 + ABZR86_RS13230 0.67 -0.4 2,971,439 + ABZR86_RS13230 0.67 +0.6 2,971,439 + ABZR86_RS13230 0.67 +0.9 2,971,439 + ABZR86_RS13230 0.67 -0.7 2,971,451 + ABZR86_RS13230 0.71 +1.3 2,971,452 - ABZR86_RS13230 0.71 -0.8 2,971,452 - ABZR86_RS13230 0.71 +0.5 2,971,452 - ABZR86_RS13230 0.71 -1.1 2,971,452 - ABZR86_RS13230 0.71 -0.0 2,971,452 - ABZR86_RS13230 0.71 +0.4 2,971,452 - ABZR86_RS13230 0.71 -0.2 2,971,633 + +0.0 2,971,634 - +0.1 2,971,641 + +0.6 2,971,641 + -4.4 2,971,641 + -1.5 2,971,642 - +0.4 2,971,642 - +0.3 2,971,796 + ABZR86_RS13235 0.45 +1.1 2,971,796 + ABZR86_RS13235 0.45 +0.6 2,971,797 - ABZR86_RS13235 0.45 +0.7 2,971,797 - ABZR86_RS13235 0.45 +0.9 2,971,982 - +0.3 2,971,982 - -0.2 2,971,996 + +0.7 2,971,997 - +0.5 2,971,997 - +0.6 2,972,141 - ABZR86_RS13240 0.15 +0.7
Or see this region's nucleotide sequence