Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS00830 and ABZR86_RS00835 are separated by 5 nucleotides ABZR86_RS00835 and ABZR86_RS00840 are separated by 50 nucleotides
ABZR86_RS00830: ABZR86_RS00830 - glucose-6-phosphate isomerase, at 160,388 to 162,025
_RS00830
ABZR86_RS00835: ABZR86_RS00835 - tRNA epoxyqueuosine(34) reductase QueG, at 162,031 to 163,104
_RS00835
ABZR86_RS00840: ABZR86_RS00840 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 163,155 to 164,639
_RS00840
Position (kb) 162
163
164 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 161.060 kb on + strand, within ABZR86_RS00830 at 161.061 kb on - strand, within ABZR86_RS00830 at 161.156 kb on + strand, within ABZR86_RS00830 at 161.156 kb on + strand, within ABZR86_RS00830 at 161.157 kb on - strand, within ABZR86_RS00830 at 161.340 kb on - strand, within ABZR86_RS00830 at 161.340 kb on - strand, within ABZR86_RS00830 at 161.387 kb on + strand, within ABZR86_RS00830 at 161.417 kb on + strand, within ABZR86_RS00830 at 161.417 kb on + strand, within ABZR86_RS00830 at 161.418 kb on - strand, within ABZR86_RS00830 at 161.537 kb on + strand, within ABZR86_RS00830 at 161.537 kb on + strand, within ABZR86_RS00830 at 161.537 kb on + strand, within ABZR86_RS00830 at 161.537 kb on + strand, within ABZR86_RS00830 at 161.538 kb on - strand, within ABZR86_RS00830 at 161.538 kb on - strand, within ABZR86_RS00830 at 161.538 kb on - strand, within ABZR86_RS00830 at 161.538 kb on - strand, within ABZR86_RS00830 at 161.566 kb on + strand, within ABZR86_RS00830 at 161.834 kb on + strand, within ABZR86_RS00830 at 161.835 kb on - strand, within ABZR86_RS00830 at 162.032 kb on + strand at 162.032 kb on + strand at 162.033 kb on - strand at 162.033 kb on - strand at 162.162 kb on + strand, within ABZR86_RS00835 at 162.163 kb on - strand, within ABZR86_RS00835 at 162.425 kb on + strand, within ABZR86_RS00835 at 162.426 kb on - strand, within ABZR86_RS00835 at 162.426 kb on - strand, within ABZR86_RS00835 at 162.426 kb on - strand, within ABZR86_RS00835 at 162.456 kb on - strand, within ABZR86_RS00835 at 162.557 kb on + strand, within ABZR86_RS00835 at 162.557 kb on + strand, within ABZR86_RS00835 at 162.557 kb on + strand, within ABZR86_RS00835 at 162.557 kb on + strand, within ABZR86_RS00835 at 162.558 kb on - strand, within ABZR86_RS00835 at 162.558 kb on - strand, within ABZR86_RS00835 at 162.578 kb on + strand, within ABZR86_RS00835 at 162.578 kb on + strand, within ABZR86_RS00835 at 162.579 kb on - strand, within ABZR86_RS00835 at 162.579 kb on - strand, within ABZR86_RS00835 at 162.579 kb on - strand, within ABZR86_RS00835 at 162.579 kb on - strand, within ABZR86_RS00835 at 162.579 kb on - strand, within ABZR86_RS00835 at 162.761 kb on + strand, within ABZR86_RS00835 at 162.761 kb on + strand, within ABZR86_RS00835 at 162.762 kb on - strand, within ABZR86_RS00835 at 162.791 kb on + strand, within ABZR86_RS00835 at 162.848 kb on + strand, within ABZR86_RS00835 at 162.848 kb on + strand, within ABZR86_RS00835 at 162.848 kb on + strand, within ABZR86_RS00835 at 162.848 kb on + strand, within ABZR86_RS00835 at 162.848 kb on + strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.932 kb on + strand, within ABZR86_RS00835 at 162.932 kb on + strand, within ABZR86_RS00835 at 162.932 kb on + strand, within ABZR86_RS00835 at 162.932 kb on + strand, within ABZR86_RS00835 at 162.932 kb on + strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 163.116 kb on + strand at 163.116 kb on + strand at 163.117 kb on - strand at 163.117 kb on - strand at 163.117 kb on - strand at 163.142 kb on + strand at 163.142 kb on + strand at 163.143 kb on - strand at 163.144 kb on + strand at 163.145 kb on - strand at 163.185 kb on + strand at 163.185 kb on + strand at 163.329 kb on + strand, within ABZR86_RS00840 at 163.542 kb on + strand, within ABZR86_RS00840 at 163.542 kb on + strand, within ABZR86_RS00840
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 161,060 + ABZR86_RS00830 0.41 -0.6 161,061 - ABZR86_RS00830 0.41 -2.1 161,156 + ABZR86_RS00830 0.47 -0.6 161,156 + ABZR86_RS00830 0.47 -0.2 161,157 - ABZR86_RS00830 0.47 -2.9 161,340 - ABZR86_RS00830 0.58 -1.7 161,340 - ABZR86_RS00830 0.58 +0.9 161,387 + ABZR86_RS00830 0.61 -0.2 161,417 + ABZR86_RS00830 0.63 -1.2 161,417 + ABZR86_RS00830 0.63 -2.7 161,418 - ABZR86_RS00830 0.63 -0.9 161,537 + ABZR86_RS00830 0.70 -1.3 161,537 + ABZR86_RS00830 0.70 -0.1 161,537 + ABZR86_RS00830 0.70 +1.7 161,537 + ABZR86_RS00830 0.70 +0.5 161,538 - ABZR86_RS00830 0.70 -4.0 161,538 - ABZR86_RS00830 0.70 -2.0 161,538 - ABZR86_RS00830 0.70 +1.7 161,538 - ABZR86_RS00830 0.70 +0.3 161,566 + ABZR86_RS00830 0.72 -0.8 161,834 + ABZR86_RS00830 0.88 -2.2 161,835 - ABZR86_RS00830 0.88 -0.5 162,032 + -0.6 162,032 + +0.7 162,033 - +0.9 162,033 - -2.6 162,162 + ABZR86_RS00835 0.12 +0.6 162,163 - ABZR86_RS00835 0.12 +0.3 162,425 + ABZR86_RS00835 0.37 -0.1 162,426 - ABZR86_RS00835 0.37 +0.9 162,426 - ABZR86_RS00835 0.37 +1.1 162,426 - ABZR86_RS00835 0.37 +0.1 162,456 - ABZR86_RS00835 0.40 +0.7 162,557 + ABZR86_RS00835 0.49 -1.5 162,557 + ABZR86_RS00835 0.49 +0.2 162,557 + ABZR86_RS00835 0.49 -1.2 162,557 + ABZR86_RS00835 0.49 -0.4 162,558 - ABZR86_RS00835 0.49 -0.5 162,558 - ABZR86_RS00835 0.49 -0.3 162,578 + ABZR86_RS00835 0.51 +0.5 162,578 + ABZR86_RS00835 0.51 +1.3 162,579 - ABZR86_RS00835 0.51 +0.5 162,579 - ABZR86_RS00835 0.51 +0.3 162,579 - ABZR86_RS00835 0.51 +0.1 162,579 - ABZR86_RS00835 0.51 +1.7 162,579 - ABZR86_RS00835 0.51 -0.5 162,761 + ABZR86_RS00835 0.68 -1.7 162,761 + ABZR86_RS00835 0.68 -0.4 162,762 - ABZR86_RS00835 0.68 -2.0 162,791 + ABZR86_RS00835 0.71 +0.2 162,848 + ABZR86_RS00835 0.76 -1.8 162,848 + ABZR86_RS00835 0.76 +0.4 162,848 + ABZR86_RS00835 0.76 -1.8 162,848 + ABZR86_RS00835 0.76 -0.8 162,848 + ABZR86_RS00835 0.76 +0.9 162,849 - ABZR86_RS00835 0.76 -0.5 162,849 - ABZR86_RS00835 0.76 +0.0 162,849 - ABZR86_RS00835 0.76 -1.9 162,849 - ABZR86_RS00835 0.76 +1.3 162,849 - ABZR86_RS00835 0.76 -0.0 162,849 - ABZR86_RS00835 0.76 +0.2 162,849 - ABZR86_RS00835 0.76 -1.5 162,849 - ABZR86_RS00835 0.76 -3.0 162,849 - ABZR86_RS00835 0.76 -1.7 162,849 - ABZR86_RS00835 0.76 -2.5 162,849 - ABZR86_RS00835 0.76 -0.4 162,932 + ABZR86_RS00835 0.84 +1.0 162,932 + ABZR86_RS00835 0.84 +0.9 162,932 + ABZR86_RS00835 0.84 +0.3 162,932 + ABZR86_RS00835 0.84 +0.0 162,932 + ABZR86_RS00835 0.84 -1.2 162,933 - ABZR86_RS00835 0.84 +0.4 162,933 - ABZR86_RS00835 0.84 -2.7 162,933 - ABZR86_RS00835 0.84 -0.5 162,933 - ABZR86_RS00835 0.84 -0.8 162,933 - ABZR86_RS00835 0.84 -0.6 162,933 - ABZR86_RS00835 0.84 +0.4 162,933 - ABZR86_RS00835 0.84 -0.1 162,933 - ABZR86_RS00835 0.84 -3.7 162,933 - ABZR86_RS00835 0.84 +0.5 162,933 - ABZR86_RS00835 0.84 -2.9 162,933 - ABZR86_RS00835 0.84 -0.4 163,116 + +1.0 163,116 + -0.2 163,117 - +1.0 163,117 - -0.1 163,117 - +0.5 163,142 + +0.7 163,142 + -0.4 163,143 - -0.9 163,144 + -0.7 163,145 - -0.3 163,185 + +0.1 163,185 + -0.3 163,329 + ABZR86_RS00840 0.12 +0.5 163,542 + ABZR86_RS00840 0.26 +1.1 163,542 + ABZR86_RS00840 0.26 +0.1
Or see this region's nucleotide sequence