Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgi and Shewana3_3149 are separated by 216 nucleotides Shewana3_3149 and Shewana3_3150 are separated by 67 nucleotides Shewana3_3150 and Shewana3_3151 are separated by 222 nucleotides Shewana3_3151 and Shewana3_3152 overlap by 8 nucleotides
Shewana3_3148: pgi - glucose-6-phosphate isomerase (RefSeq), at 3,776,328 to 3,777,965
pgi
Shewana3_3149: Shewana3_3149 - hypothetical protein (RefSeq), at 3,778,182 to 3,778,367
_3149
Shewana3_3150: Shewana3_3150 - hemerythrin HHE cation binding domain-containing protein (RefSeq), at 3,778,435 to 3,778,971
_3150
Shewana3_3151: Shewana3_3151 - hypothetical protein (RefSeq), at 3,779,194 to 3,779,901
_3151
Shewana3_3152: Shewana3_3152 - ECF subfamily RNA polymerase sigma-24 factor (RefSeq), at 3,779,894 to 3,780,496
_3152
Position (kb) 3778
3779 Strain fitness (log2 ratio) -2
-1
0
1 at 3778.218 kb on + strand, within Shewana3_3149 at 3778.448 kb on - strand at 3778.453 kb on - strand at 3778.727 kb on - strand, within Shewana3_3150 at 3778.851 kb on - strand, within Shewana3_3150 at 3779.411 kb on - strand, within Shewana3_3151 at 3779.884 kb on - strand at 3779.950 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 3,778,218 + Shewana3_3149 0.19 -0.4 3,778,448 - +1.6 3,778,453 - -2.6 3,778,727 - Shewana3_3150 0.54 -0.3 3,778,851 - Shewana3_3150 0.77 -1.2 3,779,411 - Shewana3_3151 0.31 -0.8 3,779,884 - +1.4 3,779,950 - -1.1
Or see this region's nucleotide sequence