Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3020 and Shewana3_3021 are separated by 65 nucleotides Shewana3_3021 and acpS are separated by 170 nucleotides acpS and Shewana3_3023 overlap by 1 nucleotides Shewana3_3023 and Shewana3_3024 are separated by 89 nucleotides
Shewana3_3020: Shewana3_3020 - hypothetical protein (RefSeq), at 3,626,407 to 3,627,678
_3020
Shewana3_3021: Shewana3_3021 - hypothetical protein (RefSeq), at 3,627,744 to 3,628,280
_3021
Shewana3_3022: acpS - 4'-phosphopantetheinyl transferase (RefSeq), at 3,628,451 to 3,628,834
acpS
Shewana3_3023: Shewana3_3023 - pyridoxine 5'-phosphate synthase (RefSeq), at 3,628,834 to 3,629,571
_3023
Shewana3_3024: Shewana3_3024 - DNA repair protein RecO (RefSeq), at 3,629,661 to 3,630,362
_3024
Position (kb)
3628
3629 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3627.540 kb on - strand, within Shewana3_3020 at 3627.813 kb on - strand, within Shewana3_3021 at 3628.073 kb on - strand, within Shewana3_3021 at 3628.544 kb on - strand, within acpS at 3628.589 kb on - strand, within acpS at 3628.621 kb on - strand, within acpS at 3628.646 kb on + strand, within acpS at 3628.778 kb on - strand, within acpS at 3629.629 kb on - strand at 3629.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 3,627,540 - Shewana3_3020 0.89 +0.8 3,627,813 - Shewana3_3021 0.13 -1.0 3,628,073 - Shewana3_3021 0.61 +0.5 3,628,544 - acpS Shewana3_3022 0.24 +1.3 3,628,589 - acpS Shewana3_3022 0.36 +1.6 3,628,621 - acpS Shewana3_3022 0.44 +2.8 3,628,646 + acpS Shewana3_3022 0.51 -0.2 3,628,778 - acpS Shewana3_3022 0.85 -1.5 3,629,629 - +0.5 3,629,629 - -0.5
Or see this region's nucleotide sequence