Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0095 and Shewana3_R0096 are separated by 47 nucleotides Shewana3_R0096 and Shewana3_R0097 are separated by 31 nucleotides Shewana3_R0097 and Shewana3_R0098 are separated by 46 nucleotides Shewana3_R0098 and Shewana3_R0099 are separated by 46 nucleotides Shewana3_R0099 and Shewana3_2167 are separated by 208 nucleotides Shewana3_2167 and Shewana3_2168 are separated by 125 nucleotides Shewana3_2168 and Shewana3_2169 are separated by 0 nucleotides
Shewana3_R0095: Shewana3_R0095 - tRNA-Asn (RefSeq), at 2,597,882 to 2,597,957
_R0095
Shewana3_R0096: Shewana3_R0096 - tRNA-Asn (RefSeq), at 2,598,005 to 2,598,080
_R0096
Shewana3_R0097: Shewana3_R0097 - tRNA-Asn (RefSeq), at 2,598,112 to 2,598,187
_R0097
Shewana3_R0098: Shewana3_R0098 - tRNA-Asn (RefSeq), at 2,598,234 to 2,598,309
_R0098
Shewana3_R0099: Shewana3_R0099 - tRNA-Asn (RefSeq), at 2,598,356 to 2,598,431
_R0099
Shewana3_2167: Shewana3_2167 - diguanylate cyclase/phosphodiesterase (RefSeq), at 2,598,640 to 2,600,544
_2167
Shewana3_2168: Shewana3_2168 - Na(+)-translocating NADH-quinone reductase subunit E (RefSeq), at 2,600,670 to 2,601,248
_2168
Shewana3_2169: Shewana3_2169 - electron transport complex protein RnfB (RefSeq), at 2,601,249 to 2,601,830
_2169
Position (kb)
2598
2599
2600
2601 Strain fitness (log2 ratio)
-1
0
1
2 at 2598.296 kb on + strand, within Shewana3_R0098 at 2598.472 kb on - strand at 2598.887 kb on - strand, within Shewana3_2167 at 2599.181 kb on + strand, within Shewana3_2167 at 2599.336 kb on + strand, within Shewana3_2167 at 2599.409 kb on - strand, within Shewana3_2167 at 2599.521 kb on + strand, within Shewana3_2167 at 2599.694 kb on + strand, within Shewana3_2167 at 2599.709 kb on + strand, within Shewana3_2167 at 2599.743 kb on - strand, within Shewana3_2167 at 2599.743 kb on - strand, within Shewana3_2167 at 2599.748 kb on - strand, within Shewana3_2167 at 2600.024 kb on + strand, within Shewana3_2167 at 2600.096 kb on + strand, within Shewana3_2167 at 2600.104 kb on - strand, within Shewana3_2167 at 2600.258 kb on - strand, within Shewana3_2167 at 2600.275 kb on + strand, within Shewana3_2167 at 2600.334 kb on + strand, within Shewana3_2167 at 2600.361 kb on - strand at 2600.511 kb on + strand at 2600.563 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 2,598,296 + Shewana3_R0098 0.82 +1.0 2,598,472 - +2.0 2,598,887 - Shewana3_2167 0.13 +0.4 2,599,181 + Shewana3_2167 0.28 -0.4 2,599,336 + Shewana3_2167 0.37 +1.0 2,599,409 - Shewana3_2167 0.40 +0.4 2,599,521 + Shewana3_2167 0.46 +1.0 2,599,694 + Shewana3_2167 0.55 +1.0 2,599,709 + Shewana3_2167 0.56 -1.4 2,599,743 - Shewana3_2167 0.58 +0.4 2,599,743 - Shewana3_2167 0.58 +1.5 2,599,748 - Shewana3_2167 0.58 +2.0 2,600,024 + Shewana3_2167 0.73 -0.6 2,600,096 + Shewana3_2167 0.76 -1.5 2,600,104 - Shewana3_2167 0.77 -0.8 2,600,258 - Shewana3_2167 0.85 -0.8 2,600,275 + Shewana3_2167 0.86 -0.4 2,600,334 + Shewana3_2167 0.89 -0.4 2,600,361 - +2.0 2,600,511 + +1.8 2,600,563 - -0.6
Or see this region's nucleotide sequence