Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and Shewana3_1942 are separated by 111 nucleotides Shewana3_1942 and Shewana3_1943 are separated by 2 nucleotides Shewana3_1943 and Shewana3_1944 are separated by 174 nucleotides
Shewana3_1941: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,307,669 to 2,308,784
thiH
Shewana3_1942: Shewana3_1942 - methyl-accepting chemotaxis protein (RefSeq), at 2,308,896 to 2,309,159
_1942
Shewana3_1943: Shewana3_1943 - protein-glutamate O-methyltransferase (RefSeq), at 2,309,162 to 2,311,384
_1943
Shewana3_1944: Shewana3_1944 - LysR family transcriptional regulator (RefSeq), at 2,311,559 to 2,312,461
_1944
Position (kb)
2309
2310
2311
2312 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2308.335 kb on - strand, within thiH at 2308.389 kb on - strand, within thiH at 2308.459 kb on - strand, within thiH at 2308.473 kb on - strand, within thiH at 2308.512 kb on + strand, within thiH at 2308.596 kb on - strand, within thiH at 2308.616 kb on - strand, within thiH at 2308.616 kb on - strand, within thiH at 2308.616 kb on - strand, within thiH at 2308.625 kb on - strand, within thiH at 2308.645 kb on + strand, within thiH at 2308.709 kb on + strand at 2308.987 kb on - strand, within Shewana3_1942 at 2309.379 kb on + strand at 2309.615 kb on + strand, within Shewana3_1943 at 2309.852 kb on + strand, within Shewana3_1943 at 2310.077 kb on - strand, within Shewana3_1943 at 2310.228 kb on + strand, within Shewana3_1943 at 2310.482 kb on + strand, within Shewana3_1943 at 2310.521 kb on + strand, within Shewana3_1943 at 2310.794 kb on - strand, within Shewana3_1943 at 2310.901 kb on + strand, within Shewana3_1943 at 2311.204 kb on + strand at 2311.349 kb on - strand at 2311.412 kb on - strand at 2311.425 kb on + strand at 2311.894 kb on + strand, within Shewana3_1944 at 2312.308 kb on + strand, within Shewana3_1944 at 2312.360 kb on - strand, within Shewana3_1944
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 2,308,335 - thiH Shewana3_1941 0.60 +0.5 2,308,389 - thiH Shewana3_1941 0.65 -1.4 2,308,459 - thiH Shewana3_1941 0.71 +1.1 2,308,473 - thiH Shewana3_1941 0.72 +2.1 2,308,512 + thiH Shewana3_1941 0.76 +0.2 2,308,596 - thiH Shewana3_1941 0.83 +1.1 2,308,616 - thiH Shewana3_1941 0.85 +0.5 2,308,616 - thiH Shewana3_1941 0.85 +0.5 2,308,616 - thiH Shewana3_1941 0.85 +0.1 2,308,625 - thiH Shewana3_1941 0.86 +1.1 2,308,645 + thiH Shewana3_1941 0.87 -0.9 2,308,709 + +1.3 2,308,987 - Shewana3_1942 0.34 -0.3 2,309,379 + +0.5 2,309,615 + Shewana3_1943 0.20 -0.8 2,309,852 + Shewana3_1943 0.31 -0.5 2,310,077 - Shewana3_1943 0.41 -2.8 2,310,228 + Shewana3_1943 0.48 -1.8 2,310,482 + Shewana3_1943 0.59 -1.3 2,310,521 + Shewana3_1943 0.61 -1.4 2,310,794 - Shewana3_1943 0.73 +2.0 2,310,901 + Shewana3_1943 0.78 +0.2 2,311,204 + +0.5 2,311,349 - +2.0 2,311,412 - -0.5 2,311,425 + -0.3 2,311,894 + Shewana3_1944 0.37 -0.3 2,312,308 + Shewana3_1944 0.83 +2.0 2,312,360 - Shewana3_1944 0.89 -0.3
Or see this region's nucleotide sequence