Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1869 and Shewana3_1870 are separated by 58 nucleotides Shewana3_1870 and Shewana3_1871 overlap by 1 nucleotides Shewana3_1871 and Shewana3_1872 are separated by 210 nucleotides
Shewana3_1869: Shewana3_1869 - hydrogenase expression/formation protein HypD (RefSeq), at 2,213,489 to 2,214,628
_1869
Shewana3_1870: Shewana3_1870 - hydrogenase assembly chaperone hypC/hupF (RefSeq), at 2,214,687 to 2,214,932
_1870
Shewana3_1871: Shewana3_1871 - hydrogenase accessory protein HypB (RefSeq), at 2,214,932 to 2,215,693
_1871
Shewana3_1872: Shewana3_1872 - oxidoreductase alpha (molybdopterin) subunit (RefSeq), at 2,215,904 to 2,218,225
_1872
Position (kb)
2214
2215
2216 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2214.031 kb on - strand, within Shewana3_1869 at 2214.257 kb on + strand, within Shewana3_1869 at 2214.284 kb on - strand, within Shewana3_1869 at 2214.345 kb on - strand, within Shewana3_1869 at 2214.489 kb on + strand, within Shewana3_1869 at 2214.813 kb on + strand, within Shewana3_1870 at 2215.304 kb on + strand, within Shewana3_1871 at 2215.386 kb on + strand, within Shewana3_1871 at 2215.441 kb on - strand, within Shewana3_1871 at 2215.532 kb on + strand, within Shewana3_1871 at 2215.532 kb on + strand, within Shewana3_1871 at 2215.540 kb on - strand, within Shewana3_1871 at 2215.540 kb on - strand, within Shewana3_1871 at 2215.666 kb on + strand at 2215.717 kb on - strand at 2215.900 kb on - strand at 2215.943 kb on + strand at 2215.990 kb on + strand at 2216.074 kb on + strand at 2216.089 kb on - strand at 2216.151 kb on - strand, within Shewana3_1872 at 2216.201 kb on - strand, within Shewana3_1872 at 2216.302 kb on + strand, within Shewana3_1872 at 2216.303 kb on + strand, within Shewana3_1872 at 2216.308 kb on + strand, within Shewana3_1872 at 2216.336 kb on + strand, within Shewana3_1872 at 2216.473 kb on - strand, within Shewana3_1872
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 2,214,031 - Shewana3_1869 0.48 -0.3 2,214,257 + Shewana3_1869 0.67 +2.0 2,214,284 - Shewana3_1869 0.70 +2.0 2,214,345 - Shewana3_1869 0.75 -0.2 2,214,489 + Shewana3_1869 0.88 -0.3 2,214,813 + Shewana3_1870 0.51 -0.3 2,215,304 + Shewana3_1871 0.49 +1.0 2,215,386 + Shewana3_1871 0.60 -1.0 2,215,441 - Shewana3_1871 0.67 +1.6 2,215,532 + Shewana3_1871 0.79 -0.8 2,215,532 + Shewana3_1871 0.79 +0.4 2,215,540 - Shewana3_1871 0.80 +0.2 2,215,540 - Shewana3_1871 0.80 -1.9 2,215,666 + -0.2 2,215,717 - +1.0 2,215,900 - +0.5 2,215,943 + +2.0 2,215,990 + +1.0 2,216,074 + +0.0 2,216,089 - +1.0 2,216,151 - Shewana3_1872 0.11 -2.0 2,216,201 - Shewana3_1872 0.13 +2.0 2,216,302 + Shewana3_1872 0.17 +2.0 2,216,303 + Shewana3_1872 0.17 +2.0 2,216,308 + Shewana3_1872 0.17 +0.0 2,216,336 + Shewana3_1872 0.19 +1.0 2,216,473 - Shewana3_1872 0.25 -0.4
Or see this region's nucleotide sequence