Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1625 and Shewana3_1626 are separated by 227 nucleotides Shewana3_1626 and Shewana3_1627 are separated by 18 nucleotides Shewana3_1627 and Shewana3_1628 are separated by 151 nucleotides Shewana3_1628 and Shewana3_1629 are separated by 160 nucleotides
Shewana3_1625: Shewana3_1625 - S-adenosylmethionine decarboxylase (RefSeq), at 1,914,094 to 1,915,029
_1625
Shewana3_1626: Shewana3_1626 - pyridoxamine 5'-phosphate oxidase (RefSeq), at 1,915,257 to 1,915,895
_1626
Shewana3_1627: Shewana3_1627 - hypothetical protein (RefSeq), at 1,915,914 to 1,916,366
_1627
Shewana3_1628: Shewana3_1628 - YceI family protein (RefSeq), at 1,916,518 to 1,917,093
_1628
Shewana3_1629: Shewana3_1629 - hypothetical protein (RefSeq), at 1,917,254 to 1,917,610
_1629
Position (kb)
1915
1916
1917 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1914.922 kb on - strand, within Shewana3_1625 at 1914.939 kb on + strand at 1914.982 kb on - strand at 1914.982 kb on - strand at 1915.904 kb on + strand at 1915.920 kb on - strand at 1915.922 kb on + strand at 1916.732 kb on + strand, within Shewana3_1628 at 1916.745 kb on + strand, within Shewana3_1628 at 1916.850 kb on + strand, within Shewana3_1628 at 1916.850 kb on + strand, within Shewana3_1628 at 1916.858 kb on - strand, within Shewana3_1628 at 1917.061 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 1,914,922 - Shewana3_1625 0.88 -0.2 1,914,939 + +1.2 1,914,982 - +1.9 1,914,982 - +4.1 1,915,904 + -3.0 1,915,920 - +0.9 1,915,922 + +1.9 1,916,732 + Shewana3_1628 0.37 +1.2 1,916,745 + Shewana3_1628 0.39 -0.1 1,916,850 + Shewana3_1628 0.58 -0.9 1,916,850 + Shewana3_1628 0.58 -0.1 1,916,858 - Shewana3_1628 0.59 +1.9 1,917,061 + -1.0
Or see this region's nucleotide sequence