Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1583 and Shewana3_R0069 are separated by 543 nucleotides Shewana3_R0069 and Shewana3_R0070 are separated by 57 nucleotides Shewana3_R0070 and Shewana3_R0071 are separated by 45 nucleotides Shewana3_R0071 and Shewana3_R0072 are separated by 272 nucleotides
Shewana3_1583: Shewana3_1583 - D,D-heptose 1,7-bisphosphate phosphatase (RefSeq), at 1,852,270 to 1,852,821
_1583
Shewana3_R0069: Shewana3_R0069 - 16S ribosomal RNA (RefSeq), at 1,853,365 to 1,854,919
_R0069
Shewana3_R0070: Shewana3_R0070 - tRNA-Ile (RefSeq), at 1,854,977 to 1,855,053
_R0070
Shewana3_R0071: Shewana3_R0071 - tRNA-Ala (RefSeq), at 1,855,099 to 1,855,174
_R0071
Shewana3_R0072: Shewana3_R0072 - 23S ribosomal RNA (RefSeq), at 1,855,447 to 1,858,341
_R0072
Position (kb) 1853
1854
1855 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1852.583 kb on - strand, within Shewana3_1583 at 1852.661 kb on + strand, within Shewana3_1583 at 1852.686 kb on + strand, within Shewana3_1583 at 1852.741 kb on + strand, within Shewana3_1583 at 1852.749 kb on - strand, within Shewana3_1583 at 1852.948 kb on + strand at 1852.948 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 1,852,583 - Shewana3_1583 0.57 -0.4 1,852,661 + Shewana3_1583 0.71 -0.6 1,852,686 + Shewana3_1583 0.75 -0.6 1,852,741 + Shewana3_1583 0.85 -2.0 1,852,749 - Shewana3_1583 0.87 -0.4 1,852,948 + +1.9 1,852,948 + +1.8
Or see this region's nucleotide sequence