Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0723 and Shewana3_0724 are separated by 44 nucleotides Shewana3_0724 and tolC are separated by 72 nucleotides tolC and Shewana3_0726 are separated by 243 nucleotides Shewana3_0726 and Shewana3_0727 overlap by 8 nucleotides
Shewana3_0723: Shewana3_0723 - hypothetical protein (RefSeq), at 839,748 to 840,716
_0723
Shewana3_0724: Shewana3_0724 - hypothetical protein (RefSeq), at 840,761 to 841,456
_0724
Shewana3_0725: tolC - outer membrane channel protein (RefSeq), at 841,529 to 842,836
tolC
Shewana3_0726: Shewana3_0726 - nucleoside diphosphate pyrophosphatase (RefSeq), at 843,080 to 843,691
_0726
Shewana3_0727: Shewana3_0727 - hypothetical protein (RefSeq), at 843,684 to 844,136
_0727
Position (kb) 841
842
843 Strain fitness (log2 ratio) -1
0
1
2
3 at 840.549 kb on - strand, within Shewana3_0723 at 840.593 kb on - strand, within Shewana3_0723 at 840.615 kb on + strand, within Shewana3_0723 at 840.675 kb on + strand at 840.746 kb on + strand at 840.746 kb on + strand at 840.754 kb on - strand at 840.890 kb on + strand, within Shewana3_0724 at 840.995 kb on + strand, within Shewana3_0724 at 841.054 kb on - strand, within Shewana3_0724 at 841.072 kb on + strand, within Shewana3_0724 at 841.092 kb on + strand, within Shewana3_0724 at 841.121 kb on + strand, within Shewana3_0724 at 841.220 kb on + strand, within Shewana3_0724 at 841.220 kb on + strand, within Shewana3_0724 at 841.313 kb on + strand, within Shewana3_0724 at 841.671 kb on - strand, within tolC at 841.671 kb on - strand, within tolC at 841.770 kb on - strand, within tolC at 841.839 kb on - strand, within tolC at 841.894 kb on + strand, within tolC at 842.002 kb on - strand, within tolC at 842.252 kb on - strand, within tolC at 842.494 kb on - strand, within tolC at 842.494 kb on - strand, within tolC at 842.530 kb on - strand, within tolC at 842.530 kb on - strand, within tolC at 842.530 kb on - strand, within tolC at 842.656 kb on - strand, within tolC at 842.740 kb on - strand at 842.960 kb on - strand at 842.984 kb on + strand at 843.003 kb on + strand at 843.248 kb on + strand, within Shewana3_0726 at 843.401 kb on + strand, within Shewana3_0726 at 843.462 kb on + strand, within Shewana3_0726 at 843.470 kb on - strand, within Shewana3_0726 at 843.484 kb on + strand, within Shewana3_0726 at 843.501 kb on - strand, within Shewana3_0726 at 843.510 kb on + strand, within Shewana3_0726 at 843.572 kb on + strand, within Shewana3_0726 at 843.580 kb on - strand, within Shewana3_0726 at 843.761 kb on + strand, within Shewana3_0727 at 843.791 kb on - strand, within Shewana3_0727 at 843.805 kb on + strand, within Shewana3_0727 at 843.821 kb on + strand, within Shewana3_0727 at 843.830 kb on + strand, within Shewana3_0727
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 840,549 - Shewana3_0723 0.83 +0.5 840,593 - Shewana3_0723 0.87 +1.8 840,615 + Shewana3_0723 0.89 -0.5 840,675 + +0.1 840,746 + +0.5 840,746 + +0.4 840,754 - +0.8 840,890 + Shewana3_0724 0.19 -0.3 840,995 + Shewana3_0724 0.34 +0.7 841,054 - Shewana3_0724 0.42 +0.2 841,072 + Shewana3_0724 0.45 +0.2 841,092 + Shewana3_0724 0.48 +0.2 841,121 + Shewana3_0724 0.52 +0.9 841,220 + Shewana3_0724 0.66 +0.7 841,220 + Shewana3_0724 0.66 +0.9 841,313 + Shewana3_0724 0.79 +1.7 841,671 - tolC Shewana3_0725 0.11 +2.0 841,671 - tolC Shewana3_0725 0.11 +2.0 841,770 - tolC Shewana3_0725 0.18 +2.4 841,839 - tolC Shewana3_0725 0.24 +2.6 841,894 + tolC Shewana3_0725 0.28 +1.8 842,002 - tolC Shewana3_0725 0.36 +3.0 842,252 - tolC Shewana3_0725 0.55 +2.3 842,494 - tolC Shewana3_0725 0.74 +2.9 842,494 - tolC Shewana3_0725 0.74 +1.7 842,530 - tolC Shewana3_0725 0.77 +2.3 842,530 - tolC Shewana3_0725 0.77 +2.5 842,530 - tolC Shewana3_0725 0.77 +2.9 842,656 - tolC Shewana3_0725 0.86 +2.7 842,740 - +2.9 842,960 - -0.1 842,984 + -1.2 843,003 + -0.9 843,248 + Shewana3_0726 0.27 -0.1 843,401 + Shewana3_0726 0.52 +0.9 843,462 + Shewana3_0726 0.62 +0.9 843,470 - Shewana3_0726 0.64 +1.0 843,484 + Shewana3_0726 0.66 +1.3 843,501 - Shewana3_0726 0.69 -0.2 843,510 + Shewana3_0726 0.70 -0.1 843,572 + Shewana3_0726 0.80 +0.9 843,580 - Shewana3_0726 0.82 -1.7 843,761 + Shewana3_0727 0.17 +1.0 843,791 - Shewana3_0727 0.24 +1.2 843,805 + Shewana3_0727 0.27 +2.1 843,821 + Shewana3_0727 0.30 +1.6 843,830 + Shewana3_0727 0.32 +0.7
Or see this region's nucleotide sequence