Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0599 and Shewana3_0600 are separated by 3 nucleotides Shewana3_0600 and Shewana3_0601 are separated by 368 nucleotides Shewana3_0601 and Shewana3_0602 overlap by 1 nucleotides
Shewana3_0599: Shewana3_0599 - HflK protein (RefSeq), at 702,512 to 703,657
_0599
Shewana3_0600: Shewana3_0600 - HflC protein (RefSeq), at 703,661 to 704,554
_0600
Shewana3_0601: Shewana3_0601 - ubiquinol-cytochrome c reductase, iron-sulfur subunit (RefSeq), at 704,923 to 705,513
_0601
Shewana3_0602: Shewana3_0602 - cytochrome b/b6 domain-containing protein (RefSeq), at 705,513 to 706,727
_0602
Position (kb) 703
704
705 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 702.777 kb on + strand, within Shewana3_0599 at 702.955 kb on + strand, within Shewana3_0599 at 702.992 kb on + strand, within Shewana3_0599 at 703.432 kb on + strand, within Shewana3_0599 at 703.546 kb on + strand at 703.546 kb on + strand at 703.604 kb on + strand at 704.209 kb on + strand, within Shewana3_0600 at 704.263 kb on + strand, within Shewana3_0600 at 704.481 kb on + strand at 704.829 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 702,777 + Shewana3_0599 0.23 +0.8 702,955 + Shewana3_0599 0.39 +1.8 702,992 + Shewana3_0599 0.42 +1.8 703,432 + Shewana3_0599 0.80 -0.2 703,546 + -0.5 703,546 + -2.3 703,604 + -1.2 704,209 + Shewana3_0600 0.61 -1.1 704,263 + Shewana3_0600 0.67 -3.8 704,481 + +0.3 704,829 - +1.3
Or see this region's nucleotide sequence