Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0545 and Shewana3_0546 are separated by 62 nucleotides Shewana3_0546 and Shewana3_0547 are separated by 289 nucleotides Shewana3_0547 and Shewana3_0548 are separated by 146 nucleotides
Shewana3_0545: Shewana3_0545 - alpha-L-glutamate ligase (RefSeq), at 640,850 to 641,755
_0545
Shewana3_0546: Shewana3_0546 - hypothetical protein (RefSeq), at 641,818 to 642,510
_0546
Shewana3_0547: Shewana3_0547 - histone family protein DNA-binding protein (RefSeq), at 642,800 to 643,072
_0547
Shewana3_0548: Shewana3_0548 - no description, at 643,219 to 644,016
_0548
Position (kb) 641
642
643 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 640.890 kb on - strand at 641.188 kb on - strand, within Shewana3_0545 at 641.188 kb on - strand, within Shewana3_0545 at 641.236 kb on + strand, within Shewana3_0545 at 641.486 kb on + strand, within Shewana3_0545 at 641.526 kb on + strand, within Shewana3_0545 at 641.571 kb on + strand, within Shewana3_0545 at 641.579 kb on - strand, within Shewana3_0545 at 641.655 kb on + strand, within Shewana3_0545 at 642.001 kb on + strand, within Shewana3_0546 at 642.192 kb on + strand, within Shewana3_0546 at 642.216 kb on + strand, within Shewana3_0546 at 642.258 kb on - strand, within Shewana3_0546 at 642.260 kb on + strand, within Shewana3_0546 at 642.260 kb on + strand, within Shewana3_0546 at 642.266 kb on + strand, within Shewana3_0546 at 642.358 kb on + strand, within Shewana3_0546 at 642.522 kb on + strand at 642.564 kb on + strand at 642.831 kb on + strand, within Shewana3_0547 at 642.859 kb on - strand, within Shewana3_0547 at 643.065 kb on - strand at 643.124 kb on + strand at 643.280 kb on - strand at 643.280 kb on - strand at 643.300 kb on - strand, within Shewana3_0548 at 643.388 kb on + strand, within Shewana3_0548 at 643.406 kb on - strand, within Shewana3_0548
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 640,890 - -3.4 641,188 - Shewana3_0545 0.37 +0.2 641,188 - Shewana3_0545 0.37 -0.6 641,236 + Shewana3_0545 0.43 +0.5 641,486 + Shewana3_0545 0.70 +0.2 641,526 + Shewana3_0545 0.75 -0.5 641,571 + Shewana3_0545 0.80 +0.4 641,579 - Shewana3_0545 0.80 -0.1 641,655 + Shewana3_0545 0.89 +1.0 642,001 + Shewana3_0546 0.26 -0.2 642,192 + Shewana3_0546 0.54 -0.6 642,216 + Shewana3_0546 0.57 +0.1 642,258 - Shewana3_0546 0.63 -0.1 642,260 + Shewana3_0546 0.64 -0.0 642,260 + Shewana3_0546 0.64 +1.3 642,266 + Shewana3_0546 0.65 -0.2 642,358 + Shewana3_0546 0.78 +0.3 642,522 + -0.3 642,564 + -0.8 642,831 + Shewana3_0547 0.11 -1.1 642,859 - Shewana3_0547 0.22 +2.0 643,065 - -0.7 643,124 + -2.8 643,280 - +0.5 643,280 - -0.6 643,300 - Shewana3_0548 0.10 -0.9 643,388 + Shewana3_0548 0.21 -0.4 643,406 - Shewana3_0548 0.23 -0.5
Or see this region's nucleotide sequence