Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0018 and Shewana3_R0019 are separated by 42 nucleotides Shewana3_R0019 and Shewana3_0239 are separated by 205 nucleotides Shewana3_0239 and Shewana3_0240 are separated by 38 nucleotides Shewana3_0240 and Shewana3_0241 are separated by 2 nucleotides Shewana3_0241 and Shewana3_0242 overlap by 1 nucleotides Shewana3_0242 and Shewana3_0243 overlap by 8 nucleotides
Shewana3_R0018: Shewana3_R0018 - 5S ribosomal RNA (RefSeq), at 272,229 to 272,348
_R0018
Shewana3_R0019: Shewana3_R0019 - tRNA-Asp (RefSeq), at 272,391 to 272,467
_R0019
Shewana3_0239: Shewana3_0239 - hypothetical protein (RefSeq), at 272,673 to 273,185
_0239
Shewana3_0240: Shewana3_0240 - ATPase domain-containing protein (RefSeq), at 273,224 to 274,540
_0240
Shewana3_0241: Shewana3_0241 - two component transcriptional regulator (RefSeq), at 274,543 to 275,205
_0241
Shewana3_0242: Shewana3_0242 - peptidase (RefSeq), at 275,205 to 275,471
_0242
Shewana3_0243: Shewana3_0243 - diheme cytochrome c (RefSeq), at 275,464 to 276,027
_0243
Position (kb) 273
274
275 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 273.051 kb on + strand, within Shewana3_0239 at 273.215 kb on + strand at 273.262 kb on - strand at 273.331 kb on + strand at 273.423 kb on + strand, within Shewana3_0240 at 273.431 kb on - strand, within Shewana3_0240 at 273.479 kb on - strand, within Shewana3_0240 at 273.611 kb on - strand, within Shewana3_0240 at 273.720 kb on - strand, within Shewana3_0240 at 273.825 kb on + strand, within Shewana3_0240 at 273.838 kb on - strand, within Shewana3_0240 at 273.991 kb on + strand, within Shewana3_0240 at 274.079 kb on - strand, within Shewana3_0240 at 274.126 kb on + strand, within Shewana3_0240 at 274.126 kb on + strand, within Shewana3_0240 at 274.493 kb on - strand at 274.783 kb on - strand, within Shewana3_0241 at 274.804 kb on + strand, within Shewana3_0241 at 274.843 kb on + strand, within Shewana3_0241 at 275.022 kb on - strand, within Shewana3_0241 at 275.161 kb on - strand at 275.377 kb on - strand, within Shewana3_0242 at 275.377 kb on - strand, within Shewana3_0242 at 275.377 kb on - strand, within Shewana3_0242 at 275.392 kb on + strand, within Shewana3_0242 at 275.454 kb on + strand at 275.540 kb on - strand, within Shewana3_0243
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 273,051 + Shewana3_0239 0.74 +0.5 273,215 + +0.4 273,262 - -3.3 273,331 + -0.7 273,423 + Shewana3_0240 0.15 -1.2 273,431 - Shewana3_0240 0.16 -1.0 273,479 - Shewana3_0240 0.19 +0.2 273,611 - Shewana3_0240 0.29 -0.9 273,720 - Shewana3_0240 0.38 -0.6 273,825 + Shewana3_0240 0.46 -1.5 273,838 - Shewana3_0240 0.47 +0.1 273,991 + Shewana3_0240 0.58 +0.5 274,079 - Shewana3_0240 0.65 -0.8 274,126 + Shewana3_0240 0.68 -0.7 274,126 + Shewana3_0240 0.68 +1.0 274,493 - -0.6 274,783 - Shewana3_0241 0.36 -1.2 274,804 + Shewana3_0241 0.39 -1.0 274,843 + Shewana3_0241 0.45 -0.8 275,022 - Shewana3_0241 0.72 -0.6 275,161 - +0.3 275,377 - Shewana3_0242 0.64 -0.0 275,377 - Shewana3_0242 0.64 +0.4 275,377 - Shewana3_0242 0.64 +0.3 275,392 + Shewana3_0242 0.70 -0.3 275,454 + +1.4 275,540 - Shewana3_0243 0.13 -0.9
Or see this region's nucleotide sequence