Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nudE and Shewana3_0170 are separated by 70 nucleotides Shewana3_0170 and Shewana3_0171 are separated by 6 nucleotides Shewana3_0171 and Shewana3_0172 are separated by 230 nucleotides Shewana3_0172 and Shewana3_0173 overlap by 10 nucleotides
Shewana3_0169: nudE - ADP-ribose diphosphatase NudE (RefSeq), at 202,502 to 203,053
nudE
Shewana3_0170: Shewana3_0170 - 3'(2'),5'-bisphosphate nucleotidase (RefSeq), at 203,124 to 203,936
_0170
Shewana3_0171: Shewana3_0171 - GCN5-related N-acetyltransferase (RefSeq), at 203,943 to 204,386
_0171
Shewana3_0172: Shewana3_0172 - TetR family transcriptional regulator (RefSeq), at 204,617 to 205,228
_0172
Shewana3_0173: Shewana3_0173 - phospholipid/glycerol acyltransferase (RefSeq), at 205,219 to 207,084
_0173
Position (kb) 203
204
205 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 202.978 kb on - strand, within nudE at 203.319 kb on - strand, within Shewana3_0170 at 203.319 kb on - strand, within Shewana3_0170 at 203.319 kb on - strand, within Shewana3_0170 at 203.346 kb on - strand, within Shewana3_0170 at 203.424 kb on + strand, within Shewana3_0170 at 203.685 kb on + strand, within Shewana3_0170 at 203.685 kb on + strand, within Shewana3_0170 at 203.700 kb on + strand, within Shewana3_0170 at 203.700 kb on + strand, within Shewana3_0170 at 203.824 kb on - strand, within Shewana3_0170 at 204.166 kb on - strand, within Shewana3_0171 at 204.220 kb on + strand, within Shewana3_0171 at 204.449 kb on - strand at 204.491 kb on - strand at 204.602 kb on - strand at 204.604 kb on - strand at 204.607 kb on + strand at 204.657 kb on - strand at 204.661 kb on + strand at 204.707 kb on - strand, within Shewana3_0172 at 204.707 kb on - strand, within Shewana3_0172 at 204.957 kb on + strand, within Shewana3_0172 at 205.100 kb on - strand, within Shewana3_0172
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 202,978 - nudE Shewana3_0169 0.86 -3.0 203,319 - Shewana3_0170 0.24 -1.0 203,319 - Shewana3_0170 0.24 +0.1 203,319 - Shewana3_0170 0.24 -1.7 203,346 - Shewana3_0170 0.27 -1.6 203,424 + Shewana3_0170 0.37 -0.0 203,685 + Shewana3_0170 0.69 -0.1 203,685 + Shewana3_0170 0.69 +0.8 203,700 + Shewana3_0170 0.71 +1.8 203,700 + Shewana3_0170 0.71 -2.0 203,824 - Shewana3_0170 0.86 -0.8 204,166 - Shewana3_0171 0.50 +0.4 204,220 + Shewana3_0171 0.62 +0.6 204,449 - +0.1 204,491 - +0.3 204,602 - +0.0 204,604 - -0.0 204,607 + -0.9 204,657 - -1.0 204,661 + -0.4 204,707 - Shewana3_0172 0.15 -0.8 204,707 - Shewana3_0172 0.15 +0.1 204,957 + Shewana3_0172 0.56 -0.8 205,100 - Shewana3_0172 0.79 +1.1
Or see this region's nucleotide sequence