Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4295 and Shewana3_4296 overlap by 8 nucleotides Shewana3_4296 and Shewana3_4297 are separated by 189 nucleotides Shewana3_4297 and Shewana3_4298 are separated by 28 nucleotides Shewana3_4298 and Shewana3_4299 are separated by 113 nucleotides
Shewana3_4295: Shewana3_4295 - sensor kinase CusS (RefSeq), at 178,084 to 179,559
_4295
Shewana3_4296: Shewana3_4296 - DNA-binding transcriptional activator CusR (RefSeq), at 179,552 to 180,232
_4296
Shewana3_4297: Shewana3_4297 - copper/silver efflux system outer membrane protein CusC (RefSeq), at 180,422 to 181,807
_4297
Shewana3_4298: Shewana3_4298 - periplasmic copper-binding protein (RefSeq), at 181,836 to 182,189
_4298
Shewana3_4299: Shewana3_4299 - copper/silver efflux system membrane fusion protein CusB (RefSeq), at 182,303 to 183,595
_4299
Position (kb) 180
181
182 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 179.770 kb on + strand, within Shewana3_4296 at 179.890 kb on - strand, within Shewana3_4296 at 179.911 kb on - strand, within Shewana3_4296 at 179.927 kb on + strand, within Shewana3_4296 at 179.935 kb on - strand, within Shewana3_4296 at 180.020 kb on + strand, within Shewana3_4296 at 180.178 kb on - strand at 180.318 kb on - strand at 180.444 kb on + strand at 180.691 kb on - strand, within Shewana3_4297 at 180.905 kb on + strand, within Shewana3_4297 at 181.009 kb on + strand, within Shewana3_4297 at 181.017 kb on - strand, within Shewana3_4297 at 181.017 kb on - strand, within Shewana3_4297 at 181.062 kb on + strand, within Shewana3_4297 at 181.062 kb on + strand, within Shewana3_4297 at 181.070 kb on - strand, within Shewana3_4297 at 181.352 kb on - strand, within Shewana3_4297 at 181.352 kb on - strand, within Shewana3_4297 at 181.468 kb on - strand, within Shewana3_4297 at 181.545 kb on + strand, within Shewana3_4297 at 181.563 kb on + strand, within Shewana3_4297 at 181.575 kb on + strand, within Shewana3_4297 at 181.689 kb on - strand at 181.746 kb on + strand at 181.754 kb on - strand at 181.855 kb on - strand at 182.144 kb on + strand, within Shewana3_4298 at 182.178 kb on + strand at 182.196 kb on + strand at 182.420 kb on + strand at 182.513 kb on + strand, within Shewana3_4299 at 182.565 kb on - strand, within Shewana3_4299 at 182.580 kb on + strand, within Shewana3_4299 at 182.800 kb on + strand, within Shewana3_4299
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 179,770 + Shewana3_4296 0.32 -2.0 179,890 - Shewana3_4296 0.50 +0.3 179,911 - Shewana3_4296 0.53 +1.6 179,927 + Shewana3_4296 0.55 -0.0 179,935 - Shewana3_4296 0.56 -1.3 180,020 + Shewana3_4296 0.69 -1.0 180,178 - -0.5 180,318 - +0.1 180,444 + -2.3 180,691 - Shewana3_4297 0.19 +0.2 180,905 + Shewana3_4297 0.35 -0.2 181,009 + Shewana3_4297 0.42 +0.6 181,017 - Shewana3_4297 0.43 +0.2 181,017 - Shewana3_4297 0.43 -1.4 181,062 + Shewana3_4297 0.46 -0.1 181,062 + Shewana3_4297 0.46 +0.3 181,070 - Shewana3_4297 0.47 +0.4 181,352 - Shewana3_4297 0.67 +0.4 181,352 - Shewana3_4297 0.67 -0.3 181,468 - Shewana3_4297 0.75 +0.1 181,545 + Shewana3_4297 0.81 +1.2 181,563 + Shewana3_4297 0.82 -1.2 181,575 + Shewana3_4297 0.83 +0.6 181,689 - -0.0 181,746 + +0.4 181,754 - -0.5 181,855 - -1.2 182,144 + Shewana3_4298 0.87 +0.6 182,178 + +2.8 182,196 + +0.6 182,420 + +0.1 182,513 + Shewana3_4299 0.16 +0.1 182,565 - Shewana3_4299 0.20 +0.2 182,580 + Shewana3_4299 0.21 +0.2 182,800 + Shewana3_4299 0.38 +0.6
Or see this region's nucleotide sequence