Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4159 and Shewana3_4160 are separated by 627 nucleotides Shewana3_4160 and Shewana3_4161 overlap by 4 nucleotides Shewana3_4161 and Shewana3_4162 are separated by 7 nucleotides
Shewana3_4159: Shewana3_4159 - putative transposase (RefSeq), at 21,137 to 21,307
_4159
Shewana3_4160: Shewana3_4160 - S-adenosylhomocysteine hydrolase (RefSeq), at 21,935 to 22,345
_4160
Shewana3_4161: Shewana3_4161 - hypothetical protein (RefSeq), at 22,342 to 23,283
_4161
Shewana3_4162: Shewana3_4162 - type I restriction-modification system, M subunit (RefSeq), at 23,291 to 25,915
_4162
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 21.097 kb on - strand at 21.182 kb on + strand, within Shewana3_4159 at 21.356 kb on - strand at 21.377 kb on - strand at 21.458 kb on - strand at 21.537 kb on + strand at 21.633 kb on + strand at 21.650 kb on + strand at 21.716 kb on - strand at 21.716 kb on - strand at 21.895 kb on - strand at 21.901 kb on - strand at 22.613 kb on - strand, within Shewana3_4161 at 22.766 kb on + strand, within Shewana3_4161 at 22.836 kb on + strand, within Shewana3_4161 at 22.848 kb on + strand, within Shewana3_4161 at 22.915 kb on + strand, within Shewana3_4161 at 23.000 kb on + strand, within Shewana3_4161
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 21,097 - +0.5 21,182 + Shewana3_4159 0.26 +0.8 21,356 - +1.8 21,377 - +1.1 21,458 - +0.2 21,537 + +0.2 21,633 + -0.1 21,650 + -0.9 21,716 - +0.6 21,716 - -1.6 21,895 - -0.9 21,901 - +0.7 22,613 - Shewana3_4161 0.29 -2.6 22,766 + Shewana3_4161 0.45 -0.1 22,836 + Shewana3_4161 0.52 +0.4 22,848 + Shewana3_4161 0.54 -1.4 22,915 + Shewana3_4161 0.61 -0.5 23,000 + Shewana3_4161 0.70 -1.3
Or see this region's nucleotide sequence