Experiment: Ceftazidime 5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1024 and Shew_1025 overlap by 4 nucleotides Shew_1025 and Shew_1026 are separated by 115 nucleotides Shew_1026 and Shew_1027 are separated by 37 nucleotides
Shew_1024: Shew_1024 - hypothetical protein (RefSeq), at 1,183,237 to 1,183,857
_1024
Shew_1025: Shew_1025 - hypothetical protein (RefSeq), at 1,183,854 to 1,184,243
_1025
Shew_1026: Shew_1026 - cobalamin biosynthesis protein CbiB (RefSeq), at 1,184,359 to 1,185,342
_1026
Shew_1027: Shew_1027 - methylthioadenosine nucleosidase (RefSeq), at 1,185,380 to 1,186,072
_1027
Position (kb)
1184
1185
1186 Strain fitness (log2 ratio)
-1
0
1 at 1183.373 kb on - strand, within Shew_1024 at 1183.373 kb on - strand, within Shew_1024 at 1183.588 kb on + strand, within Shew_1024 at 1183.750 kb on + strand, within Shew_1024 at 1183.925 kb on + strand, within Shew_1025 at 1183.925 kb on + strand, within Shew_1025 at 1184.490 kb on - strand, within Shew_1026 at 1184.562 kb on - strand, within Shew_1026 at 1184.768 kb on + strand, within Shew_1026 at 1186.100 kb on - strand at 1186.157 kb on + strand at 1186.266 kb on + strand at 1186.307 kb on + strand at 1186.307 kb on + strand at 1186.310 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ceftazidime 5 mg/ml remove 1,183,373 - Shew_1024 0.22 +0.6 1,183,373 - Shew_1024 0.22 -0.0 1,183,588 + Shew_1024 0.57 +0.2 1,183,750 + Shew_1024 0.83 -0.4 1,183,925 + Shew_1025 0.18 +1.5 1,183,925 + Shew_1025 0.18 +0.3 1,184,490 - Shew_1026 0.13 -0.8 1,184,562 - Shew_1026 0.21 +0.2 1,184,768 + Shew_1026 0.42 -0.9 1,186,100 - -1.6 1,186,157 + -0.3 1,186,266 + -0.0 1,186,307 + -1.1 1,186,307 + +0.2 1,186,310 - -0.1
Or see this region's nucleotide sequence