Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_629 and Pf6N2E2_630 overlap by 8 nucleotides Pf6N2E2_630 and Pf6N2E2_631 are separated by 63 nucleotides
Pf6N2E2_629 - 2-ketogluconate kinase (EC 2.7.1.13), at 716,617 to 717,591
_629
Pf6N2E2_630 - Epimerase KguE, at 717,584 to 718,366
_630
Pf6N2E2_631 - 2-ketogluconate utilization repressor PtxS, at 718,430 to 719,455
_631
Position (kb) 717
718
719 Strain fitness (log2 ratio) -2
-1
0
1 at 716.888 kb on + strand, within Pf6N2E2_629 at 716.889 kb on - strand, within Pf6N2E2_629 at 716.889 kb on - strand, within Pf6N2E2_629 at 717.272 kb on + strand, within Pf6N2E2_629 at 717.272 kb on + strand, within Pf6N2E2_629 at 717.272 kb on + strand, within Pf6N2E2_629 at 717.698 kb on + strand, within Pf6N2E2_630 at 717.699 kb on - strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.814 kb on - strand, within Pf6N2E2_630 at 717.814 kb on - strand, within Pf6N2E2_630 at 718.071 kb on - strand, within Pf6N2E2_630 at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.371 kb on - strand at 718.371 kb on - strand at 718.373 kb on + strand at 718.373 kb on + strand at 718.634 kb on + strand, within Pf6N2E2_631 at 718.634 kb on + strand, within Pf6N2E2_631 at 718.635 kb on - strand, within Pf6N2E2_631 at 718.635 kb on - strand, within Pf6N2E2_631 at 719.083 kb on - strand, within Pf6N2E2_631 at 719.083 kb on - strand, within Pf6N2E2_631 at 719.083 kb on - strand, within Pf6N2E2_631 at 719.152 kb on + strand, within Pf6N2E2_631 at 719.152 kb on + strand, within Pf6N2E2_631 at 719.153 kb on - strand, within Pf6N2E2_631 at 719.153 kb on - strand, within Pf6N2E2_631 at 719.153 kb on - strand, within Pf6N2E2_631
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 716,888 + Pf6N2E2_629 0.28 +0.4 716,889 - Pf6N2E2_629 0.28 +0.7 716,889 - Pf6N2E2_629 0.28 -1.2 717,272 + Pf6N2E2_629 0.67 -0.0 717,272 + Pf6N2E2_629 0.67 +0.3 717,272 + Pf6N2E2_629 0.67 -0.2 717,698 + Pf6N2E2_630 0.15 +0.5 717,699 - Pf6N2E2_630 0.15 -0.9 717,813 + Pf6N2E2_630 0.29 -0.5 717,813 + Pf6N2E2_630 0.29 +0.5 717,813 + Pf6N2E2_630 0.29 -0.6 717,814 - Pf6N2E2_630 0.29 -0.7 717,814 - Pf6N2E2_630 0.29 -0.1 718,071 - Pf6N2E2_630 0.62 -2.6 718,370 + -0.6 718,370 + -0.3 718,370 + +0.6 718,370 + -0.3 718,370 + -1.7 718,371 - -0.1 718,371 - -0.5 718,373 + +0.6 718,373 + +0.9 718,634 + Pf6N2E2_631 0.20 +0.1 718,634 + Pf6N2E2_631 0.20 -1.1 718,635 - Pf6N2E2_631 0.20 -0.2 718,635 - Pf6N2E2_631 0.20 -0.4 719,083 - Pf6N2E2_631 0.64 +0.4 719,083 - Pf6N2E2_631 0.64 +0.1 719,083 - Pf6N2E2_631 0.64 +0.4 719,152 + Pf6N2E2_631 0.70 -0.4 719,152 + Pf6N2E2_631 0.70 +1.0 719,153 - Pf6N2E2_631 0.70 -0.1 719,153 - Pf6N2E2_631 0.70 -0.2 719,153 - Pf6N2E2_631 0.70 -0.5
Or see this region's nucleotide sequence