Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4794 and Pf6N2E2_4795 are separated by 8 nucleotides Pf6N2E2_4795 and Pf6N2E2_4796 are separated by 83 nucleotides Pf6N2E2_4796 and Pf6N2E2_4797 overlap by 10 nucleotides Pf6N2E2_4797 and Pf6N2E2_4798 are separated by 79 nucleotides
Pf6N2E2_4794 - TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 5,543,816 to 5,544,841
_4794
Pf6N2E2_4795 - Acyl-phosphate:glycerol-3-phosphate O-acyltransferase PlsY, at 5,544,850 to 5,545,419
_4795
Pf6N2E2_4796 - Dihydroneopterin aldolase (EC 4.1.2.25), at 5,545,503 to 5,545,847
_4796
Pf6N2E2_4797 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 5,545,838 to 5,546,356
_4797
Pf6N2E2_4798 - tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25), at 5,546,436 to 5,547,662
_4798
Position (kb)
5545
5546
5547 Strain fitness (log2 ratio)
-2
-1
0
1 at 5544.851 kb on + strand at 5544.886 kb on + strand at 5544.886 kb on + strand at 5544.886 kb on + strand at 5544.887 kb on - strand at 5544.938 kb on - strand, within Pf6N2E2_4795 at 5545.025 kb on + strand, within Pf6N2E2_4795 at 5545.025 kb on + strand, within Pf6N2E2_4795 at 5545.026 kb on - strand, within Pf6N2E2_4795 at 5545.133 kb on + strand, within Pf6N2E2_4795 at 5545.134 kb on - strand, within Pf6N2E2_4795 at 5545.144 kb on + strand, within Pf6N2E2_4795 at 5545.144 kb on + strand, within Pf6N2E2_4795 at 5545.144 kb on + strand, within Pf6N2E2_4795 at 5545.145 kb on - strand, within Pf6N2E2_4795 at 5545.145 kb on - strand, within Pf6N2E2_4795 at 5545.422 kb on + strand at 5545.422 kb on + strand at 5545.422 kb on + strand at 5545.422 kb on + strand at 5545.422 kb on + strand at 5545.423 kb on - strand at 5545.426 kb on + strand at 5545.452 kb on + strand at 5545.452 kb on + strand at 5546.129 kb on + strand, within Pf6N2E2_4797 at 5546.130 kb on - strand, within Pf6N2E2_4797 at 5546.335 kb on + strand at 5546.335 kb on + strand at 5546.335 kb on + strand at 5546.335 kb on + strand at 5546.399 kb on - strand at 5546.399 kb on - strand at 5546.454 kb on - strand at 5546.455 kb on + strand at 5546.455 kb on + strand at 5546.455 kb on + strand at 5546.456 kb on - strand at 5546.456 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,544,851 + -0.7 5,544,886 + +0.7 5,544,886 + +0.5 5,544,886 + +0.2 5,544,887 - -0.8 5,544,938 - Pf6N2E2_4795 0.15 -0.2 5,545,025 + Pf6N2E2_4795 0.31 -0.6 5,545,025 + Pf6N2E2_4795 0.31 -0.4 5,545,026 - Pf6N2E2_4795 0.31 -0.3 5,545,133 + Pf6N2E2_4795 0.50 -2.6 5,545,134 - Pf6N2E2_4795 0.50 +0.0 5,545,144 + Pf6N2E2_4795 0.52 +0.9 5,545,144 + Pf6N2E2_4795 0.52 -0.4 5,545,144 + Pf6N2E2_4795 0.52 +0.6 5,545,145 - Pf6N2E2_4795 0.52 -0.0 5,545,145 - Pf6N2E2_4795 0.52 -0.2 5,545,422 + -0.6 5,545,422 + +0.2 5,545,422 + -0.2 5,545,422 + -0.5 5,545,422 + +0.0 5,545,423 - -0.3 5,545,426 + +0.4 5,545,452 + +0.4 5,545,452 + -0.7 5,546,129 + Pf6N2E2_4797 0.56 -1.6 5,546,130 - Pf6N2E2_4797 0.56 -0.1 5,546,335 + +0.7 5,546,335 + +0.2 5,546,335 + -0.4 5,546,335 + -0.6 5,546,399 - -0.9 5,546,399 - -0.1 5,546,454 - +0.3 5,546,455 + -0.5 5,546,455 + +1.3 5,546,455 + +0.8 5,546,456 - -0.3 5,546,456 - +0.3
Or see this region's nucleotide sequence