Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_477 and Pf6N2E2_478 are separated by 283 nucleotides Pf6N2E2_478 and Pf6N2E2_479 are separated by 3 nucleotides
Pf6N2E2_477 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 542,197 to 543,216
_477
Pf6N2E2_478 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data) , at 543,500 to 544,885
_478
Pf6N2E2_479 - Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data) , at 544,889 to 546,160
_479
Position (kb)
543
544
545 Strain fitness (log2 ratio)
-2
-1
0
1 at 542.652 kb on - strand, within Pf6N2E2_477 at 542.747 kb on + strand, within Pf6N2E2_477 at 542.843 kb on + strand, within Pf6N2E2_477 at 543.173 kb on + strand at 543.174 kb on - strand at 543.174 kb on - strand at 543.226 kb on + strand at 543.226 kb on + strand at 543.227 kb on - strand at 543.227 kb on - strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.501 kb on + strand at 544.195 kb on - strand, within Pf6N2E2_478 at 544.195 kb on - strand, within Pf6N2E2_478 at 544.341 kb on + strand, within Pf6N2E2_478 at 544.342 kb on - strand, within Pf6N2E2_478 at 544.830 kb on + strand at 544.891 kb on - strand at 545.075 kb on + strand, within Pf6N2E2_479 at 545.497 kb on - strand, within Pf6N2E2_479 at 545.599 kb on - strand, within Pf6N2E2_479 at 545.599 kb on - strand, within Pf6N2E2_479 at 545.643 kb on + strand, within Pf6N2E2_479 at 545.644 kb on - strand, within Pf6N2E2_479 at 545.644 kb on - strand, within Pf6N2E2_479
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 542,652 - Pf6N2E2_477 0.45 +0.2 542,747 + Pf6N2E2_477 0.54 -1.4 542,843 + Pf6N2E2_477 0.63 +0.0 543,173 + +0.7 543,174 - +0.4 543,174 - +0.6 543,226 + -1.9 543,226 + +0.1 543,227 - +0.5 543,227 - -1.0 543,288 + -1.3 543,288 + +0.7 543,288 + +0.2 543,288 + +0.3 543,288 + -0.8 543,288 + -0.3 543,289 - +0.1 543,289 - +0.4 543,289 - -0.1 543,289 - +0.4 543,289 - +0.2 543,289 - +0.2 543,289 - -0.6 543,289 - +0.3 543,289 - -0.5 543,501 + +0.3 544,195 - Pf6N2E2_478 0.50 -1.3 544,195 - Pf6N2E2_478 0.50 +0.3 544,341 + Pf6N2E2_478 0.61 -0.7 544,342 - Pf6N2E2_478 0.61 -0.1 544,830 + -2.5 544,891 - +0.6 545,075 + Pf6N2E2_479 0.15 -0.1 545,497 - Pf6N2E2_479 0.48 -0.7 545,599 - Pf6N2E2_479 0.56 -0.5 545,599 - Pf6N2E2_479 0.56 +0.8 545,643 + Pf6N2E2_479 0.59 -0.0 545,644 - Pf6N2E2_479 0.59 +0.2 545,644 - Pf6N2E2_479 0.59 +0.4
Or see this region's nucleotide sequence