Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4615

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4613 and Pf6N2E2_4614 are separated by 48 nucleotidesPf6N2E2_4614 and Pf6N2E2_4615 are separated by 10 nucleotidesPf6N2E2_4615 and Pf6N2E2_4616 are separated by 158 nucleotides Pf6N2E2_4613 - twitching motility protein PilG, at 5,338,004 to 5,338,504 _4613 Pf6N2E2_4614 - twitching motility protein PilH, at 5,338,553 to 5,338,918 _4614 Pf6N2E2_4615 - type IV pili signal transduction protein PilI, at 5,338,929 to 5,339,468 _4615 Pf6N2E2_4616 - type IV pilus biogenesis protein PilJ, at 5,339,627 to 5,341,690 _4616 Position (kb) 5338 5339 5340Strain fitness (log2 ratio) -1 0 1at 5337.984 kb on + strandat 5338.047 kb on + strandat 5338.595 kb on + strand, within Pf6N2E2_4614at 5338.595 kb on + strand, within Pf6N2E2_4614at 5338.595 kb on + strand, within Pf6N2E2_4614at 5338.596 kb on - strand, within Pf6N2E2_4614at 5338.853 kb on + strand, within Pf6N2E2_4614at 5338.854 kb on - strand, within Pf6N2E2_4614at 5338.854 kb on - strand, within Pf6N2E2_4614at 5338.854 kb on - strand, within Pf6N2E2_4614at 5338.854 kb on - strand, within Pf6N2E2_4614at 5338.854 kb on - strand, within Pf6N2E2_4614at 5338.854 kb on - strand, within Pf6N2E2_4614at 5339.067 kb on + strand, within Pf6N2E2_4615at 5339.068 kb on - strand, within Pf6N2E2_4615at 5339.528 kb on + strandat 5339.528 kb on + strandat 5339.612 kb on + strandat 5339.613 kb on - strandat 5339.742 kb on - strandat 5339.762 kb on + strandat 5339.763 kb on - strandat 5339.774 kb on + strandat 5339.774 kb on + strandat 5339.774 kb on + strandat 5339.775 kb on - strandat 5339.775 kb on - strandat 5339.775 kb on - strandat 5340.214 kb on + strand, within Pf6N2E2_4616at 5340.214 kb on + strand, within Pf6N2E2_4616at 5340.215 kb on - strand, within Pf6N2E2_4616

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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5,337,984 + -0.2
5,338,047 + +0.0
5,338,595 + Pf6N2E2_4614 0.11 +0.3
5,338,595 + Pf6N2E2_4614 0.11 +0.9
5,338,595 + Pf6N2E2_4614 0.11 -0.5
5,338,596 - Pf6N2E2_4614 0.12 -0.6
5,338,853 + Pf6N2E2_4614 0.82 -1.5
5,338,854 - Pf6N2E2_4614 0.82 +0.9
5,338,854 - Pf6N2E2_4614 0.82 -0.8
5,338,854 - Pf6N2E2_4614 0.82 -0.3
5,338,854 - Pf6N2E2_4614 0.82 +0.6
5,338,854 - Pf6N2E2_4614 0.82 +0.5
5,338,854 - Pf6N2E2_4614 0.82 -0.5
5,339,067 + Pf6N2E2_4615 0.26 -0.8
5,339,068 - Pf6N2E2_4615 0.26 -0.2
5,339,528 + +0.3
5,339,528 + -0.2
5,339,612 + +0.4
5,339,613 - +0.1
5,339,742 - +0.2
5,339,762 + +0.1
5,339,763 - +0.3
5,339,774 + +0.2
5,339,774 + -0.2
5,339,774 + +0.5
5,339,775 - +0.3
5,339,775 - -0.2
5,339,775 - -0.5
5,340,214 + Pf6N2E2_4616 0.28 +0.9
5,340,214 + Pf6N2E2_4616 0.28 -0.2
5,340,215 - Pf6N2E2_4616 0.28 +0.5

Or see this region's nucleotide sequence