Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4589 and Pf6N2E2_4590 are separated by 54 nucleotides Pf6N2E2_4590 and Pf6N2E2_4591 are separated by 9 nucleotides Pf6N2E2_4591 and Pf6N2E2_4592 are separated by 144 nucleotides Pf6N2E2_4592 and Pf6N2E2_4593 are separated by 21 nucleotides
Pf6N2E2_4589 - Sulfur carrier protein ThiS, at 5,318,825 to 5,319,043
_4589
Pf6N2E2_4590 - Thiazole biosynthesis protein ThiG, at 5,319,098 to 5,319,892
_4590
Pf6N2E2_4591 - tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33), at 5,319,902 to 5,320,627
_4591
Pf6N2E2_4592 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 5,320,772 to 5,321,095
_4592
Pf6N2E2_4593 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 5,321,117 to 5,322,250
_4593
Position (kb)
5319
5320
5321 Strain fitness (log2 ratio)
-1
0
1 at 5319.142 kb on + strand at 5319.142 kb on + strand at 5319.143 kb on - strand at 5319.180 kb on - strand, within Pf6N2E2_4590 at 5319.314 kb on + strand, within Pf6N2E2_4590 at 5319.515 kb on + strand, within Pf6N2E2_4590 at 5319.515 kb on + strand, within Pf6N2E2_4590 at 5319.515 kb on + strand, within Pf6N2E2_4590 at 5319.516 kb on - strand, within Pf6N2E2_4590 at 5319.516 kb on - strand, within Pf6N2E2_4590 at 5319.516 kb on - strand, within Pf6N2E2_4590 at 5319.624 kb on - strand, within Pf6N2E2_4590 at 5319.624 kb on - strand, within Pf6N2E2_4590 at 5319.821 kb on + strand at 5319.852 kb on - strand at 5319.890 kb on + strand at 5319.890 kb on + strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.458 kb on - strand, within Pf6N2E2_4591 at 5320.458 kb on - strand, within Pf6N2E2_4591 at 5320.466 kb on + strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.626 kb on - strand at 5320.690 kb on + strand at 5320.690 kb on + strand at 5320.691 kb on - strand at 5320.691 kb on - strand at 5320.716 kb on + strand at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.931 kb on + strand, within Pf6N2E2_4592 at 5321.115 kb on - strand at 5321.118 kb on + strand at 5321.118 kb on + strand at 5321.118 kb on + strand at 5321.119 kb on - strand at 5321.119 kb on - strand at 5321.119 kb on - strand at 5321.119 kb on - strand at 5321.119 kb on - strand at 5321.388 kb on + strand, within Pf6N2E2_4593 at 5321.388 kb on + strand, within Pf6N2E2_4593 at 5321.388 kb on + strand, within Pf6N2E2_4593 at 5321.389 kb on - strand, within Pf6N2E2_4593 at 5321.420 kb on + strand, within Pf6N2E2_4593 at 5321.421 kb on - strand, within Pf6N2E2_4593 at 5321.496 kb on + strand, within Pf6N2E2_4593 at 5321.496 kb on + strand, within Pf6N2E2_4593 at 5321.497 kb on - strand, within Pf6N2E2_4593 at 5321.506 kb on + strand, within Pf6N2E2_4593 at 5321.550 kb on + strand, within Pf6N2E2_4593
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,319,142 + -0.0 5,319,142 + +0.7 5,319,143 - -0.4 5,319,180 - Pf6N2E2_4590 0.10 -0.2 5,319,314 + Pf6N2E2_4590 0.27 +0.5 5,319,515 + Pf6N2E2_4590 0.52 +1.0 5,319,515 + Pf6N2E2_4590 0.52 -0.1 5,319,515 + Pf6N2E2_4590 0.52 +0.1 5,319,516 - Pf6N2E2_4590 0.53 -0.4 5,319,516 - Pf6N2E2_4590 0.53 -0.6 5,319,516 - Pf6N2E2_4590 0.53 +0.4 5,319,624 - Pf6N2E2_4590 0.66 -0.1 5,319,624 - Pf6N2E2_4590 0.66 +0.5 5,319,821 + -0.1 5,319,852 - +1.7 5,319,890 + +0.8 5,319,890 + +0.1 5,319,891 - +0.6 5,319,891 - +0.4 5,319,891 - -0.5 5,319,891 - +0.2 5,319,891 - -0.8 5,319,891 - +0.4 5,320,457 + Pf6N2E2_4591 0.76 +0.8 5,320,457 + Pf6N2E2_4591 0.76 +0.7 5,320,457 + Pf6N2E2_4591 0.76 -0.5 5,320,457 + Pf6N2E2_4591 0.76 -1.5 5,320,458 - Pf6N2E2_4591 0.77 -1.4 5,320,458 - Pf6N2E2_4591 0.77 -1.3 5,320,466 + Pf6N2E2_4591 0.78 -1.0 5,320,467 - Pf6N2E2_4591 0.78 +0.6 5,320,467 - Pf6N2E2_4591 0.78 -1.2 5,320,467 - Pf6N2E2_4591 0.78 +0.3 5,320,467 - Pf6N2E2_4591 0.78 -0.6 5,320,467 - Pf6N2E2_4591 0.78 +0.5 5,320,467 - Pf6N2E2_4591 0.78 -0.4 5,320,467 - Pf6N2E2_4591 0.78 -0.8 5,320,626 - +0.5 5,320,690 + -0.4 5,320,690 + +0.2 5,320,691 - +1.0 5,320,691 - +0.6 5,320,716 + -1.6 5,320,836 + Pf6N2E2_4592 0.20 +0.5 5,320,836 + Pf6N2E2_4592 0.20 +0.4 5,320,836 + Pf6N2E2_4592 0.20 +0.4 5,320,836 + Pf6N2E2_4592 0.20 -0.5 5,320,836 + Pf6N2E2_4592 0.20 +0.2 5,320,836 + Pf6N2E2_4592 0.20 +0.1 5,320,837 - Pf6N2E2_4592 0.20 +0.5 5,320,837 - Pf6N2E2_4592 0.20 -0.7 5,320,837 - Pf6N2E2_4592 0.20 +0.2 5,320,837 - Pf6N2E2_4592 0.20 +0.8 5,320,837 - Pf6N2E2_4592 0.20 +0.3 5,320,837 - Pf6N2E2_4592 0.20 -0.4 5,320,837 - Pf6N2E2_4592 0.20 +0.0 5,320,837 - Pf6N2E2_4592 0.20 -0.2 5,320,881 + Pf6N2E2_4592 0.34 +0.5 5,320,881 + Pf6N2E2_4592 0.34 -0.1 5,320,881 + Pf6N2E2_4592 0.34 +0.2 5,320,882 - Pf6N2E2_4592 0.34 -0.8 5,320,882 - Pf6N2E2_4592 0.34 +0.3 5,320,882 - Pf6N2E2_4592 0.34 +0.1 5,320,882 - Pf6N2E2_4592 0.34 +1.2 5,320,931 + Pf6N2E2_4592 0.49 +0.2 5,321,115 - +0.7 5,321,118 + +1.0 5,321,118 + +1.0 5,321,118 + -0.8 5,321,119 - +0.2 5,321,119 - +0.4 5,321,119 - +0.0 5,321,119 - -0.5 5,321,119 - +0.7 5,321,388 + Pf6N2E2_4593 0.24 +0.1 5,321,388 + Pf6N2E2_4593 0.24 +0.5 5,321,388 + Pf6N2E2_4593 0.24 -0.0 5,321,389 - Pf6N2E2_4593 0.24 +0.2 5,321,420 + Pf6N2E2_4593 0.27 +0.2 5,321,421 - Pf6N2E2_4593 0.27 -0.3 5,321,496 + Pf6N2E2_4593 0.33 +1.0 5,321,496 + Pf6N2E2_4593 0.33 -0.2 5,321,497 - Pf6N2E2_4593 0.34 +0.0 5,321,506 + Pf6N2E2_4593 0.34 -0.4 5,321,550 + Pf6N2E2_4593 0.38 +0.0
Or see this region's nucleotide sequence