Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3655

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3653 and Pf6N2E2_3654 are separated by 85 nucleotidesPf6N2E2_3654 and Pf6N2E2_3655 are separated by 268 nucleotidesPf6N2E2_3655 and Pf6N2E2_3656 are separated by 16 nucleotidesPf6N2E2_3656 and Pf6N2E2_3657 overlap by 4 nucleotides Pf6N2E2_3653 - ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase, at 4,253,445 to 4,254,866 _3653 Pf6N2E2_3654 - Adenylylsulfate kinase (EC 2.7.1.25), at 4,254,952 to 4,255,413 _3654 Pf6N2E2_3655 - Hypothetical protein FIG015671 in large core OS assembly cluster, at 4,255,682 to 4,257,097 _3655 Pf6N2E2_3656 - Probable transcription regulator Mig-14, at 4,257,114 to 4,258,013 _3656 Pf6N2E2_3657 - Lipid A export ATP-binding/permease protein MsbA, at 4,258,010 to 4,259,800 _3657 Position (kb) 4255 4256 4257 4258Strain fitness (log2 ratio) -3 -2 -1 0 1at 4254.881 kb on - strandat 4254.906 kb on - strandat 4254.906 kb on - strandat 4254.906 kb on - strandat 4254.906 kb on - strandat 4254.906 kb on - strandat 4255.046 kb on + strand, within Pf6N2E2_3654at 4255.047 kb on - strand, within Pf6N2E2_3654at 4255.100 kb on + strand, within Pf6N2E2_3654at 4255.151 kb on + strand, within Pf6N2E2_3654at 4255.151 kb on + strand, within Pf6N2E2_3654at 4255.151 kb on + strand, within Pf6N2E2_3654at 4255.152 kb on - strand, within Pf6N2E2_3654at 4255.152 kb on - strand, within Pf6N2E2_3654at 4255.330 kb on + strand, within Pf6N2E2_3654at 4255.330 kb on + strand, within Pf6N2E2_3654at 4255.330 kb on + strand, within Pf6N2E2_3654at 4255.330 kb on + strand, within Pf6N2E2_3654at 4255.331 kb on - strand, within Pf6N2E2_3654at 4255.331 kb on - strand, within Pf6N2E2_3654at 4255.378 kb on + strandat 4255.379 kb on - strandat 4255.452 kb on + strandat 4255.453 kb on - strandat 4255.453 kb on - strandat 4255.453 kb on - strandat 4255.521 kb on + strandat 4255.540 kb on + strandat 4255.624 kb on + strandat 4255.624 kb on + strandat 4255.626 kb on + strandat 4255.634 kb on + strandat 4255.653 kb on + strandat 4255.653 kb on + strandat 4255.654 kb on - strandat 4255.654 kb on - strandat 4255.866 kb on - strand, within Pf6N2E2_3655at 4255.916 kb on + strandat 4256.016 kb on + strand, within Pf6N2E2_3655at 4256.017 kb on - strand, within Pf6N2E2_3655at 4256.017 kb on - strand, within Pf6N2E2_3655at 4256.017 kb on - strand, within Pf6N2E2_3655at 4256.017 kb on - strand, within Pf6N2E2_3655at 4256.029 kb on + strand, within Pf6N2E2_3655at 4256.044 kb on + strand, within Pf6N2E2_3655at 4256.045 kb on - strand, within Pf6N2E2_3655at 4256.045 kb on - strand, within Pf6N2E2_3655at 4256.160 kb on - strand, within Pf6N2E2_3655at 4256.160 kb on - strand, within Pf6N2E2_3655at 4256.248 kb on + strand, within Pf6N2E2_3655at 4256.249 kb on - strand, within Pf6N2E2_3655at 4256.249 kb on - strand, within Pf6N2E2_3655at 4256.255 kb on - strand, within Pf6N2E2_3655at 4256.322 kb on + strand, within Pf6N2E2_3655at 4256.322 kb on + strand, within Pf6N2E2_3655at 4256.322 kb on + strand, within Pf6N2E2_3655at 4256.322 kb on + strand, within Pf6N2E2_3655at 4256.322 kb on + strand, within Pf6N2E2_3655at 4256.323 kb on - strand, within Pf6N2E2_3655at 4256.323 kb on - strand, within Pf6N2E2_3655at 4256.323 kb on - strand, within Pf6N2E2_3655at 4256.325 kb on + strand, within Pf6N2E2_3655at 4256.448 kb on + strand, within Pf6N2E2_3655at 4256.449 kb on - strand, within Pf6N2E2_3655at 4256.449 kb on - strand, within Pf6N2E2_3655at 4256.453 kb on - strand, within Pf6N2E2_3655at 4256.489 kb on - strand, within Pf6N2E2_3655at 4256.510 kb on - strand, within Pf6N2E2_3655at 4256.511 kb on + strand, within Pf6N2E2_3655at 4256.512 kb on - strand, within Pf6N2E2_3655at 4256.512 kb on - strand, within Pf6N2E2_3655at 4256.512 kb on - strand, within Pf6N2E2_3655at 4256.512 kb on - strand, within Pf6N2E2_3655at 4256.560 kb on + strand, within Pf6N2E2_3655at 4256.560 kb on + strand, within Pf6N2E2_3655at 4256.747 kb on + strand, within Pf6N2E2_3655at 4256.748 kb on - strand, within Pf6N2E2_3655at 4256.748 kb on - strand, within Pf6N2E2_3655at 4256.896 kb on - strand, within Pf6N2E2_3655at 4256.896 kb on - strand, within Pf6N2E2_3655at 4256.901 kb on + strand, within Pf6N2E2_3655at 4256.901 kb on + strand, within Pf6N2E2_3655at 4256.919 kb on + strand, within Pf6N2E2_3655at 4256.919 kb on + strand, within Pf6N2E2_3655at 4256.919 kb on + strand, within Pf6N2E2_3655at 4256.919 kb on + strand, within Pf6N2E2_3655at 4256.920 kb on - strand, within Pf6N2E2_3655at 4256.920 kb on - strand, within Pf6N2E2_3655at 4256.920 kb on - strand, within Pf6N2E2_3655at 4256.920 kb on - strand, within Pf6N2E2_3655at 4256.920 kb on - strand, within Pf6N2E2_3655at 4256.920 kb on - strand, within Pf6N2E2_3655at 4257.042 kb on - strandat 4257.124 kb on + strandat 4257.124 kb on + strandat 4257.157 kb on + strandat 4257.157 kb on + strandat 4257.301 kb on + strand, within Pf6N2E2_3656at 4257.301 kb on + strand, within Pf6N2E2_3656at 4257.301 kb on + strand, within Pf6N2E2_3656at 4257.301 kb on + strand, within Pf6N2E2_3656at 4257.301 kb on + strand, within Pf6N2E2_3656at 4257.373 kb on + strand, within Pf6N2E2_3656at 4257.478 kb on + strand, within Pf6N2E2_3656at 4257.478 kb on + strand, within Pf6N2E2_3656at 4257.478 kb on + strand, within Pf6N2E2_3656at 4257.478 kb on + strand, within Pf6N2E2_3656at 4257.478 kb on + strand, within Pf6N2E2_3656at 4257.478 kb on + strand, within Pf6N2E2_3656at 4257.515 kb on + strand, within Pf6N2E2_3656at 4257.515 kb on + strand, within Pf6N2E2_3656at 4257.515 kb on + strand, within Pf6N2E2_3656at 4257.584 kb on + strand, within Pf6N2E2_3656at 4257.584 kb on + strand, within Pf6N2E2_3656at 4257.589 kb on + strand, within Pf6N2E2_3656at 4257.589 kb on + strand, within Pf6N2E2_3656at 4257.673 kb on + strand, within Pf6N2E2_3656at 4257.673 kb on + strand, within Pf6N2E2_3656at 4257.673 kb on + strand, within Pf6N2E2_3656at 4257.673 kb on + strandat 4257.673 kb on + strand, within Pf6N2E2_3656at 4257.673 kb on + strand, within Pf6N2E2_3656at 4257.856 kb on + strand, within Pf6N2E2_3656at 4257.949 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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4,254,881 - +0.5
4,254,906 - +0.8
4,254,906 - +0.2
4,254,906 - +0.4
4,254,906 - -0.1
4,254,906 - -0.4
4,255,046 + Pf6N2E2_3654 0.20 +0.1
4,255,047 - Pf6N2E2_3654 0.21 -0.3
4,255,100 + Pf6N2E2_3654 0.32 +0.2
4,255,151 + Pf6N2E2_3654 0.43 -0.7
4,255,151 + Pf6N2E2_3654 0.43 -0.2
4,255,151 + Pf6N2E2_3654 0.43 -0.5
4,255,152 - Pf6N2E2_3654 0.43 +0.1
4,255,152 - Pf6N2E2_3654 0.43 +0.1
4,255,330 + Pf6N2E2_3654 0.82 -0.2
4,255,330 + Pf6N2E2_3654 0.82 +0.6
4,255,330 + Pf6N2E2_3654 0.82 -0.1
4,255,330 + Pf6N2E2_3654 0.82 +0.2
4,255,331 - Pf6N2E2_3654 0.82 +0.5
4,255,331 - Pf6N2E2_3654 0.82 +0.3
4,255,378 + +0.3
4,255,379 - +0.3
4,255,452 + -0.1
4,255,453 - +1.4
4,255,453 - +0.6
4,255,453 - +0.3
4,255,521 + +0.0
4,255,540 + -0.5
4,255,624 + +1.3
4,255,624 + -0.2
4,255,626 + -0.1
4,255,634 + +0.2
4,255,653 + -0.1
4,255,653 + +0.4
4,255,654 - -1.1
4,255,654 - -0.8
4,255,866 - Pf6N2E2_3655 0.13 +0.1
4,255,916 + +0.9
4,256,016 + Pf6N2E2_3655 0.24 -1.5
4,256,017 - Pf6N2E2_3655 0.24 +0.1
4,256,017 - Pf6N2E2_3655 0.24 +0.3
4,256,017 - Pf6N2E2_3655 0.24 +0.0
4,256,017 - Pf6N2E2_3655 0.24 -1.0
4,256,029 + Pf6N2E2_3655 0.25 +0.5
4,256,044 + Pf6N2E2_3655 0.26 -0.5
4,256,045 - Pf6N2E2_3655 0.26 -0.4
4,256,045 - Pf6N2E2_3655 0.26 -0.2
4,256,160 - Pf6N2E2_3655 0.34 -0.8
4,256,160 - Pf6N2E2_3655 0.34 -0.8
4,256,248 + Pf6N2E2_3655 0.40 -0.0
4,256,249 - Pf6N2E2_3655 0.40 -0.7
4,256,249 - Pf6N2E2_3655 0.40 +0.1
4,256,255 - Pf6N2E2_3655 0.40 -0.5
4,256,322 + Pf6N2E2_3655 0.45 +0.3
4,256,322 + Pf6N2E2_3655 0.45 +0.5
4,256,322 + Pf6N2E2_3655 0.45 -0.3
4,256,322 + Pf6N2E2_3655 0.45 +0.1
4,256,322 + Pf6N2E2_3655 0.45 +0.3
4,256,323 - Pf6N2E2_3655 0.45 +0.2
4,256,323 - Pf6N2E2_3655 0.45 -1.7
4,256,323 - Pf6N2E2_3655 0.45 -0.1
4,256,325 + Pf6N2E2_3655 0.45 -0.6
4,256,448 + Pf6N2E2_3655 0.54 -0.7
4,256,449 - Pf6N2E2_3655 0.54 +0.2
4,256,449 - Pf6N2E2_3655 0.54 -0.2
4,256,453 - Pf6N2E2_3655 0.54 -0.5
4,256,489 - Pf6N2E2_3655 0.57 +0.7
4,256,510 - Pf6N2E2_3655 0.58 -1.1
4,256,511 + Pf6N2E2_3655 0.59 -1.5
4,256,512 - Pf6N2E2_3655 0.59 -0.9
4,256,512 - Pf6N2E2_3655 0.59 -1.0
4,256,512 - Pf6N2E2_3655 0.59 -1.3
4,256,512 - Pf6N2E2_3655 0.59 +0.1
4,256,560 + Pf6N2E2_3655 0.62 -1.4
4,256,560 + Pf6N2E2_3655 0.62 -0.2
4,256,747 + Pf6N2E2_3655 0.75 -0.8
4,256,748 - Pf6N2E2_3655 0.75 -1.7
4,256,748 - Pf6N2E2_3655 0.75 -0.6
4,256,896 - Pf6N2E2_3655 0.86 -0.7
4,256,896 - Pf6N2E2_3655 0.86 -0.7
4,256,901 + Pf6N2E2_3655 0.86 -0.6
4,256,901 + Pf6N2E2_3655 0.86 +0.1
4,256,919 + Pf6N2E2_3655 0.87 -0.1
4,256,919 + Pf6N2E2_3655 0.87 -0.7
4,256,919 + Pf6N2E2_3655 0.87 -1.3
4,256,919 + Pf6N2E2_3655 0.87 -3.1
4,256,920 - Pf6N2E2_3655 0.87 +0.7
4,256,920 - Pf6N2E2_3655 0.87 -0.4
4,256,920 - Pf6N2E2_3655 0.87 -1.1
4,256,920 - Pf6N2E2_3655 0.87 -0.8
4,256,920 - Pf6N2E2_3655 0.87 -0.3
4,256,920 - Pf6N2E2_3655 0.87 -0.2
4,257,042 - -0.5
4,257,124 + -1.8
4,257,124 + -0.7
4,257,157 + -1.7
4,257,157 + -0.2
4,257,301 + Pf6N2E2_3656 0.21 -2.8
4,257,301 + Pf6N2E2_3656 0.21 +0.0
4,257,301 + Pf6N2E2_3656 0.21 -0.5
4,257,301 + Pf6N2E2_3656 0.21 -0.5
4,257,301 + Pf6N2E2_3656 0.21 -1.1
4,257,373 + Pf6N2E2_3656 0.29 -2.1
4,257,478 + Pf6N2E2_3656 0.40 -0.1
4,257,478 + Pf6N2E2_3656 0.40 -0.2
4,257,478 + Pf6N2E2_3656 0.40 -0.5
4,257,478 + Pf6N2E2_3656 0.40 +0.3
4,257,478 + Pf6N2E2_3656 0.40 -0.4
4,257,478 + Pf6N2E2_3656 0.40 +1.0
4,257,515 + Pf6N2E2_3656 0.45 -1.5
4,257,515 + Pf6N2E2_3656 0.45 -1.6
4,257,515 + Pf6N2E2_3656 0.45 -1.9
4,257,584 + Pf6N2E2_3656 0.52 +0.4
4,257,584 + Pf6N2E2_3656 0.52 -1.4
4,257,589 + Pf6N2E2_3656 0.53 +0.5
4,257,589 + Pf6N2E2_3656 0.53 -0.4
4,257,673 + Pf6N2E2_3656 0.62 -1.1
4,257,673 + Pf6N2E2_3656 0.62 -1.6
4,257,673 + Pf6N2E2_3656 0.62 -0.8
4,257,673 + +1.3
4,257,673 + Pf6N2E2_3656 0.62 -1.4
4,257,673 + Pf6N2E2_3656 0.62 -0.1
4,257,856 + Pf6N2E2_3656 0.82 -0.3
4,257,949 + -0.8

Or see this region's nucleotide sequence